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Takashi SUGIMURA, Kotaro KOYAMA, Masahiko WATANABE, Keiji WAKABAYASHI
Article type: Article
2004 Volume 78 Issue 5-6 Pages
271-281
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
JOURNAL
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NAD serves as a substrate for the ADP-ribosylation of proteins. In addition to the poly ADP-ribosylation of histones and other nuclear proteins by the poly (ADP-ribose) polymerase (PARP) family, mono-ADP-ribosylation reactions are catalyzed by bacterial toxins. Interestingly, the capacity is shared by pierisin-1, a peptide isolated from the late larval and early pupal stages of cabbage butterfly (Pieris rapae), which shows potent apoptogenic activity against mammalian cells. Pierisin-1 was purified as 100 kDa protein and a cDNA was cloned and in vitro synthesis achieved in a rabbit reticulocyte lysate system. Purified pierisin-1 caused 50% growth inhibition of cultured HeLa cells at only 0.043 ng/ml. The protein features an N-terminal domain with an ADP-ribosylating sequence and a C-terminal domain with binding capacity to Gb3 and Gb4 on cell surfaces, and apoptogenic activity was found to be lost on replacement of glutamic acid 165 by glutamine. Pierisin-1 ADP-ribosylated DNA but not proteins with NAD as the substrate, indicating unique ADP-ribosyltransferase activity, and transfer of ADP-ribosyl moiety to 2-amino-group of deoxyguanosine could be confirmed. Forced expression of bcl-2 in cells suppressed apoptosis caused by pierisin-1, Cytochrome c release and activation of caspases 9 and 3 were evident. At low concentration of pierisin-1 not causing apoptosis, mutations leading to G→C transversions were induced in cultured mammalian cells. The distribution of pierisin is limited to genus Pieris butterflies and mRNA and pierisin activity demonstrate peaks during the late larval and early pupal stages. The physiological function of pierisin for cabbage butterflies remains to be unveiled. A carcinostatic activity has been demonstrated in vivo.
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[in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
283-286
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
286-288
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
288-290
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
291-293
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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Article type: Bibliography
2004 Volume 78 Issue 5-6 Pages
295-299
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
301-302
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
302-
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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Violeta G. Reneva, Hiroyuki Shimasaki
Article type: Article
2004 Volume 78 Issue 5-6 Pages
302-303
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
303-
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
304-
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
304-
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
305-306
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
306-307
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
2004 Volume 78 Issue 5-6 Pages
308-
Published: June 25, 2004
Released on J-STAGE: October 10, 2017
JOURNAL
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