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Tohru Yoshimura
Article type: Article
2015 Volume 89 Issue 7 Pages
327-340
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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The reaction mechanism of vitamin B_6 (pyridoxal 5'-phosphate)-dependent enzymes involved in the amino acid metabolism have been studied for over 60 years. Some of our contributions to this scientific field have been introduced in this review. This review has focused on the stereospecificity for the proton transfer between Cα of the substrate amino acid and C4' of the cofactor upon the half transamination catalyzed by various pyridoxal enzymes. We found the enzymes that catalyze the C-4' pro-R hydrogen transfer and the C-4' pro-S and pro-R hydrogen transfer for the first time. We confirmed that the stereospecificty is related to the structure and molecular evolution of the enzymes. Now, D-Amino acids such as D-serine and D-aspartate attract attention because of their physiological functions in the central nerve system and so on. We compared the reaction mechanism of pyridoxal 5'-phosphate-dependent alanine racemase and serine racemase, which are responsible for the de novo synthesis of D-amino acids. Both enzyme reactions proceed through a similar two-base mechanism, though the enzymes are structurally and evolutionally different. We found a novel D-serine dehydratase, which is a pyridoxal enzyme and responsible for D-serine degradation in vertebrates except mammals. The structure, function, and application of the enzyme have also been introduced in this review.
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Hiroko Horiuchi, Naoki Harada, Ryoichi Yamaji
Article type: Article
2015 Volume 89 Issue 7 Pages
341-347
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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Yoshiki Koriyama, Kayo Sugitani, Kazuhiro Ogai, Satoru Kato
Article type: Article
2015 Volume 89 Issue 7 Pages
348-350
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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Koji Fukui
Article type: Article
2015 Volume 89 Issue 7 Pages
351-353
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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Shoji Yamada, Keiko Yamada
Article type: Article
2015 Volume 89 Issue 7 Pages
354-357
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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[in Japanese]
Article type: Article
2015 Volume 89 Issue 7 Pages
358-359
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
2015 Volume 89 Issue 7 Pages
359-360
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
2015 Volume 89 Issue 7 Pages
361-362
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
2015 Volume 89 Issue 7 Pages
362-
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
2015 Volume 89 Issue 7 Pages
363-
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
2015 Volume 89 Issue 7 Pages
363-364
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
FREE ACCESS
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[in Japanese]
Article type: Article
2015 Volume 89 Issue 7 Pages
364-365
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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[in Japanese]
Article type: Article
2015 Volume 89 Issue 7 Pages
365-
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
FREE ACCESS
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[in Japanese]
Article type: Article
2015 Volume 89 Issue 7 Pages
365-366
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
FREE ACCESS
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Article type: Bibliography
2015 Volume 89 Issue 7 Pages
367-372
Published: July 25, 2015
Released on J-STAGE: August 10, 2017
JOURNAL
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