アレルギー
Online ISSN : 1347-7935
Print ISSN : 0021-4884
ISSN-L : 0021-4884
9 巻, 3 号
選択された号の論文の10件中1~10を表示しています
  • 原稿種別: 目次
    1960 年 9 巻 3 号 p. 1008-1011
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
  • 菅原 孝雄
    原稿種別: 本文
    1960 年 9 巻 3 号 p. 231-238
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
    The amount of antigen and antibody required for the development of Arthus reaction was studied in guinea pigs using ovalbumin-antiovalbumin system. After the provocative injections of the antigen intradermally to the sensitized guinea pigs, the amounts of antigen as well as antibody in the local sites were persued using these reagents labelled with ^<131>I. The antigen/antibody ratio in the sites was estimated from these results. The antibody concentration in the site increased immediately after the injections of the antigen, while, a part of the antigen injected disappeared from the site, combining with antibody in the circulation and in the tissues. The development of the Arthus reaction was closely related to the amount of antibody in the site present at 4 hours after the antigen injection, when hemorrage appears in positive reactions, but was irrespective of either the amount of tissue antibody before the antigen injection or the antigen/antibody ratio in the sites immediately after the injection. When the hemorrage appeared, the amount of antibody in the reactive sites was more than 1.2 μg N and the antigen/antibody ratio in these sites was either in equivalence or in the region of excess antigen. These findings suggested the possibility that the Arthus reaction might be induced when the total amounts of antigen and antibody reacted in the site reached to a certain extent.
  • 鈴木 廣造
    原稿種別: 本文
    1960 年 9 巻 3 号 p. 239-245
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
    Rabbits weighing 2.5 kg were actively sensitized with 2% egg-albumin in order to study the significance of histamine releaser in the mechanism of the development of Arthus phenomenon. Histamine releaser, 50γ of compound 48/80 or 500γ of sinomenin, were injected intracutaneously for 5 days successively. Extraction of histamine was done following the modified method by Dwetch and Loew and qualitatively determined by the biological technique. Results obtained indicate that the histamine content of the skin decreased to 44-71% by the treatment of compound 48/80 and 53-63% by the treatment of sinomenin. The histamine content in the same sites recovered to 79-80% 65 days after the treatment of histamine releaser and 120 days after the treatment it almost recovered to the initial level. On the other hand, histamine content in the skin developed Arthus phenomenon was observed to be increased remarkably and it was also observed that Arthus phenomenon have been developed in the skin depleted histament by the treatment of histamine releaser and histamine content in the same site of the skin was found to be increased. These facts suggest that histamine may have some relation to the development of Arthus phenomenon.
  • 大沢 武夫
    原稿種別: 本文
    1960 年 9 巻 3 号 p. 246-251
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
    Cellular toxin seems to be produced by injecting the homogenate of the homolgous and heterologous tissues and it has been said that the function and resistance of the cells might be decreased when a large number of the cellular toxin were produced. In this experiment the author tried to examine whether the resistance is decreased or not by determinating the opsonin index when the homogenated homologous and heterologous tissues were injected. For examining the homologous cellular toxin the tissue homogenate of mice were injected to mice and for the experimemts of the heterologous cellular toxin the tissue homogenate of mice and, rats were injected to the rabbits. The results obtained showed that the homologous and heterologous serum opsonin titer decreased by injecting the tissue homogenate, in spite of the tissues were homologous or heterologous and the tissue opsonin titer was lower than the serum opsonin titer by injecting the heterologous tissue. It was also found that the heterologous cellular toxic injuring action was more severe than the homologus one.
  • 新井 義夫, 鈴木 廣造, 六川 俊雄
    原稿種別: 本文
    1960 年 9 巻 3 号 p. 252-258
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
    Guinea-pigs were passively sensitized with anti-egg-albumin rabbit serum. Twenty hours after the sensitization they were killed by heart puncture and the lung, small-intestine and liver were extracted and suspended in Tyrode solution at 37℃. These materials were divided into two groups; one was control and the other was for anaphylaxis in vitro. Two percent of egg-albumin was added into the latter groups and shocked in vitro. The biological activity and chemical components of the supernatants by centrifugation were examined. As the chemical components appeared in the medium during anaphylaxis in vitro histamine and serotonin were mainly tested. Histamine extraction was followed by Dwetch and Loew's method (1954) by cotton acid succinate and determined quantitatively by the biological technique, and serotonin was extracted by modified method described by Udenfriend and Weissbach et al (1955) and quantitatively determined by ultraviolet absorption. The solutions obtained during anaphylaxis in vitro showed more severe contracting action of the guinea pig's intestine than the control solutions. However the results obtained with the antihistamine and antiacetylcholine drug suggested that histamine, acetylcholine and serotonin might be produced during anaphylaxis in vitro. Namely, histamine in the lung's solution during anaphylaxis in vitro was proved to be increased about 2 times and serotonin was 3 times than the control solution. Histamine in the liver's solution during anaphylaxis in vitro was also found to be increased about 50% and serotonin about 2 times. Serotonin value in the case of the small intestine was about 50% higher than the control solution, but not a remarkble change was recognized in histamine value. The findings that histamine and serotonin might be increased during anaphylaxis in vitro were clearly demonstrated by the chemical analysis.
  • 直木 由太郎
    原稿種別: 本文
    1960 年 9 巻 3 号 p. 259-274
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
    Shock of the brain was induced by injecting the bovine erythrocytes into the carotid artery, other arteries and veins of the previously sensitized rabbits and the histological changes in the brain caused by this active anaphylaxis were investigated. The most severe histological changes were observed in the rabbits injected provocatively into the carotid artery, and the findings observed in the rabbits injected provocatively into other arteries and veins were rather milder. In the control group, in which rabbits were sensitized and not injected provocatively, no significant changes were detected. The histopathological findings of the central nervous system of rabbits injected provocatively were the focal and the diffuse glial proliferation, the degeneration of ganglion cells, the infil tration of perivascular cells, congestion, hemorrhage and several degrees of edema. Besides-these changes, the lucid plaque which is the synonym of the patch of necrosis, are found in the brain tissues of the rabbits injected provocatively into the carotid artery.
  • 増村 雄二郎
    原稿種別: 本文
    1960 年 9 巻 3 号 p. 275-284
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
    The tuberculin tests were repeated 2 times at the same site and the temporal patterns of the reactions were observed. The reaction in the new site of the tuberculous school children was a little stronger than that of the healthy school children, and the reactions in both cases were observed to be maximum at 48th hour after the injection. On the repeated site, the early reaction was observed to be increased at 24th hour after the injection. The reaction at the repeated site was far more remarkable than in the new site and it was far more remarkable in the tuberculous children than in the healthy school children. The difference in the grade of the reaction between healthy and tuberculous children due to the grade of the tuberculin Sensitization and the change of the tuberculin reaction by the repeated injection seemed to depend upon not only the local but also the general sensitization.
  • 高橋 恒尹
    原稿種別: 本文
    1960 年 9 巻 3 号 p. 285-294
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
    To investigate the relation between the temporal pattern of the tuberculin reaction and the circulating antibody, 21 healthy and the circulating antibody, 21 healthy and 15 tuberculous cases were used for the tests. The tuberculin reaction was performed as described in the previous reports, and the circulating antibody was determined by hemoaggulutination, hemolysis, Boyden's technique and Shindo-Utahashi's method. The fraction extracted from the non-heated tuberculin was used as the antigen. In the healthy cases, the tuberculin reaction rarely showed acceration, and the circulating antibody was frequently accerated, and the antibody was positive ofener than in the healthy cases. In the cases repeated tuberculin reaction tended to be accerated and the antibody titers was ofen elevated. From the relation between the pattern of the tuberculin reaction and the circulating antibody, it was clarified that the antibody titer tended to be higher in the cases whom reaction was accerated. It was known that the substance absorbed from the tuberculous cavity and the tuberculin inoculation may produce the change to tuberculin allergy in the living body and increase the circulating antibody and accerate tuberculin reaction.
  • 東条 順治
    原稿種別: 本文
    1960 年 9 巻 3 号 p. 295-305
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
    The relation between the plasmin activation and the decrease of leucocytes, especially on the direct effect of the active plasmin against leucocytes were investigated and at the same time, the mechanism of the decrease of leucocytes in anaphylaxis were also studied from the point of plasmin activation. When active plasmin extracted from the blood plasma of the dog was injected into the rabbit, the decrease of the leucocytes, chiefly of granular leucocytes were observed to be developed. When Plasmin was acted directly to leucocytes in vitro, granular leucocytes decreased in number. When plasmin was added to the separated lencocytes and incubated, various changes, such as the concentration and fusion of nucleus, appearance of the small vacuoles both in the cell body and in the nucleus were observed. Even when plasmin was liberated upon marrow leucocytes not only in vitro bur also in culture, the decrease in number or any change of the leucocytes could not be observed. It was clarified that anti-plasmin could suppress the decrease of leucocytes by adding the active plasmin. In the case of anapylaxis of the rabbit sensitized with egg-albumin, the activation of plasmin and the decrease of the granular leucocytes were usually observed, expect a few cases.
  • 原稿種別: 文献目録等
    1960 年 9 巻 3 号 p. 305-308
    発行日: 1960/03/01
    公開日: 2016/10/20
    ジャーナル フリー
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