アレルギー
Online ISSN : 1347-7935
Print ISSN : 0021-4884
ISSN-L : 0021-4884
18 巻, 1 号
選択された号の論文の14件中1~14を表示しています
  • 原稿種別: 表紙
    1969 年 18 巻 1 号 p. Cover1-
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原稿種別: 表紙
    1969 年 18 巻 1 号 p. Cover2-
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 藤本 重義
    原稿種別: Article
    1969 年 18 巻 1 号 p. 1-15,86
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Independent from the recent publication of Johnson and Vaughan (1967), investigations had been made about canine antibodies. Seven immunoglobulins each possessing antigenically distinct heavy chain and common light chain were identified and their structure and sequence of antibody responses were examined. Hyperimmunized dog serum with bovine serum albumin (BSA) demonstrated at least seven different radioactive bands with I^<131>-labelled antigen in radioimmunoelectrophoresis. All of these precipitin bands also reacted with I^<131>-labelled anti-dog L chain. Partial purification of each immunoglobulin was performed by the combination of sodium sulfate precipitation, DEAE-cellulose column chromatography and gel-filtration on Sephadex G200 or Sepharose 4B column. Antigenic structure and antibody activities were examined by immunodiffusion and radioimmunoelectrophoresis techniques. In immunoelectrophoresis and radioimmunoelectrophoresis three cross-reactive precipitin bands (γG_1, γG_2 and γG_3) with antibody activity appeared in γ_2 region. All three showed cross-reactivity with human γG globulin and were considered to be subclasses of dog γG globulin. Purified γG globulin had sedimentation coefficient (S_<20, W>) of 6.7S in analytical ultracentrifugation. In papain digestion and 2-mercaptoethanol treatment, dog γG globulin showed comparable chain structure with human or rabbit γG globulin. Four precipitin bands each possessing antibody activity were found in γ_1 region each having distinct H chain and common L chain. One of them with 17.6S (S_<20, W>) and cross-reactive with human γM globulin equalled to γM globulin of dog. Among other three γ1 globulins, major immunoglobulin showed sdimentation coefficient of 6.6S and was designated γ_<la> globulin. This protein showed no cross-reactivity with human γG, γA, γM and D-myeloma protein and was considered to be a unique immunoglobulin in dog. The other two minor γ1 globulins (γ_1b, γ_1c) were eluted a little faster from Sephadex G200 column than γG or γ_1a and considered to have slightly larger molecular size. The sequence of antibody response to BSA in these seven immunoglobulins was examined by radioimmunoelectrophoresis. The antibody activity was first appeared in γG_1 and γG_2 precipitin bands, next in γG_3 and γ_la. The activity of γG class decreased whereas that of γ_1a increased according to prolongation of immunization and became a major antibody in the hyperimmune period. Antibody activity of hyperimmunized dog serum with BSA was mainly in γ_la globulin and this antiserum did not show distinct precipitin reaction and passive hemagglutination. Nevertheless it had been proved this antiserum had the activities to inhibit the precipitin reaction and passive hemagglutination of rabbit antibody to BSA at high dilution.
  • 可部 順三郎, 宮本 昭正
    原稿種別: 本文
    1969 年 18 巻 1 号 p. 16-25,87
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    The experiments were designed to analyse the reactions of the respiratory tract of guinea pigs sensitized with egg albumin (EA) or tubercle bacilli following the exposure to the antigens. Oscillation method was used to record the changes of respiratory frequency and respiratory resistance of the animals. In the experiments of the reactions of immediate type, 39 guinea pigs inhaled aerosolized EA after passive sensitization with γ_1 or γ_2 globulin fraction of anti-EA guinea pig serum. To separate γ_1 and γ_2 globulin fraction the serum was applied to a column of DEAE cellulose, equilibrated with sodium phosphate buffer 0.005 M of 0.15 M at pH 8.0. The purity of both fractions was examined by PCA and passive lysis. Guinea pigs sensitized with anti-EA antibodies reacted with immediate increase of respiratory resistance following the exposure to the antigens. The magnitude of the reactions was of the following order; 1) pigs which were challenged 24 hrs. after sensitization with γ_l. 2) pigs challenged within 1 hr. of sensitization with γ_2. 3) pigs challenged within 1 hr. of sensitization with γ_1. 4) Pigs challenged 24 hrs. after sensitization with γ2. The resistance returned to normal in one hour except in the second group in which the increase of the resistance lasted for 2-4 hrs. There was no definite change in respiratory frequency in any of the above groups. Of the guinea pigs sensitized with tubercle bacilli, immediate increase of respiratory resistance was noted in some of the pigs which were exposed to old tuberculin spray but in none exposed to PPD. At 24 hrs. after exposure, all these animals exhibited 1.5-2 times higher respiratory frequency than those of pre-treatment. This tachypnea was not accompanied by the change in respiratory resistance and was not noted after old tuberculin inhalation in the control group. It was concluded that the biological active substance which produces bronchospasm upon exposure to the antigen was contained both in γ_1 and in γ_2 globulin fraction of guinea pigs. It seemed that the sub stance in γ_1 fraction required the latent period for the fixation to tissue but the substance in γ_2 fraction did not. Allergic reactions of Arthus type and of delayed type were not likely to produce bronchospasm although the latter caused dyspnea with distinct tachypnea of the guinea pigs. Histological studies support this view.
  • 進藤 宙二, 伊藤 幸治, 大城 晶子, 白石 聰
    原稿種別: 本文
    1969 年 18 巻 1 号 p. 26-32,87-88
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    1) The immunogenicity of NH-ACTH extracted from the pituitary glands of pigs and synthetic ACTH (tetracosactide) consisted of 24 amino acids was investigated in rabbits. 2) Two of five sera of the rabbits immunized with NH-ACTH gave positive PCA reaction to NH-ACTH, and reacted with I^<131>-labelled NH-ACTH in the radioimmunoelectrophoresis. One rabbit died in a state of anaphylactic shock. 3) No antibody against tetracosactide was detected by the PCA and radioimmunoelectrophoresis in the sera of the rabbits immunized with tetracosactide. 4) The tetracosctide was non-reactive with the antiNH-ACTH antibody in the PCA and radioimmunoelectrophoresis. 5) It seems, therefore, that tetracosactide (β^<1-24> ACTH) is not immunogenic in rabbits.
  • 進藤 宙二, 伊藤 幸治, 大城 晶子, 白石 聰
    原稿種別: 本文
    1969 年 18 巻 1 号 p. 33-34,88
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    1) The Antigenicity of tetracosactide (β^<1-24> ACTH) was investigated in guinea pigs. 2) Three of five guinea pigs immunized with tetracosactide showed signs of anaphylactic shock, though the sera were not reacted with tetracosactide in the PCA and radioimmunoelectrophoresis.
  • 渡辺 信一郎
    原稿種別: 本文
    1969 年 18 巻 1 号 p. 35-45,88
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Rabbit IgG antibodies against bacterial α-amylase were treated with trypsin in a medium free of any added reducing agent. The 5S fragments were isolated from the tryptic digest by fractionation with ammonium sulfate followed by gel filtration through Sephadex G-100. The tryptic 5S fragments could (I) form specific precipitate with corresponding antigen, (2) neutralizeamylase activity, (3) react with antiFab but not with anti-Fc sera, (4) fail to induce passive cutaneous anaphylaxis in guinea pigs. With these respects the tryptic 5S fragments were identified as divalent antibody fragments and were designated as F(ab")_2. The F(ab")_2 fragments were split into the 3.5S univalent fragments. The 3.5S fragments were also obtained by tryptic digestion in the presence of cysteine. The 3.5S univalent fragments closely resembled the papain 3.5S Fab fragments according to the antigenic analysis using goat antir-abbit globulin, Fab and Fc sera, and were designated as Fab" Homoreactants against Fab" or F(ab")_2 fragments from rabbit IgG could be demonstrated in the normal rabbit IgG by passive hemagglutination method. These homoreactants could be distinguished from homoreactants against Fab and Fab' reported by Mandy, W.J. The homoreactants against Fab" and Fab were partially purified with immunoadsorbents which were made by conjugating with Fab, Fab', Fab" insoluble poly-RSA by means of bis-diazotized benzidine method. These purified homoreactants showed specific binding activities with homologous immunoadsorbents. These activities were inhibited by homologous fragments but not or little by heterologous fragments. From these results, it is clear that antigenic differences among Fab, Fab', Fab" and F (ab")_2 can be demonstrated by the homoreactants present in normal rabbit serum.
  • 長沢 俊彦, 宮川 侑三, 成清 卓二, 柴田 整一
    原稿種別: 本文
    1969 年 18 巻 1 号 p. 46-55,89
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    There are several reports that renal medulla contains nephrotoxic antigen, which were mainly confirmed by in vitro studies, such as absorption or precipitation procedures. However, the localization of nephrotoxic antigen in medulla is still obscure and the different results were reported on the production of nephrotoxic serum nephritis (NTN) using antiserum against renal medulla. In this paper, tryptic digested ultrasupernatant of rat inner medulla (TR-M) were used as an antigen for the production of NTN, because the active principle of nephrotoxic antigen in glomerular basement membrane (GBM) is proved to be present in the tryptic digested ultrasupernatant of renal cortex. TR-M, as the antigen, could produce more potent nephrotoxic antiserum than simple emulsion of medulla, if the medulla contained nephrotoxic antigen. The antiserum against TR-M was obtained immunizing rabbits by the alum precipitation method. The injection of anti TR-M could produce typical NTN both clinically and histologically; Proteinuria from 50 to 100 mg/day appeared next day after the injection and continued during the observation period of I to 60 days. Histologic examination showed severe glomerulitis in all rats tested, but tubules in cortex and medulla showed no primary changes attributable to the injetion of antiserum except marked endothelial cell proliferation of vasa recta in outer medulla in several rats. The injection of FITC labeled anti TR-M revealed that all the antisera injected fixed only in GBM. The immunofluorescent staining of normal rat kidney with FITC labeled anti TR-M showed the following specific localization of anti TR-M. I) Cortex; GBM, Bowman's capsule, tubular BM and media of small blood vessels. II) Outer medulla; tubular BM and capillary BM of vasa recta. III) Inner medulla; tubular BM and capillary BM. Tubular BM and capillary BM was easily differentiated in outer medulla, but was very diffiuclt in inner medulla. From these studies it is apparent that antisera against TR-M have an ability to produce NTN, and the nephrotoxic antigen in medulla exists in tubular and/or capillary BM. The nephrotoxic antigenicity of capillary BM is already well known, however, the relationship between tubular BM and nephrotoxic antigenicity needs further elucidation.
  • 我妻 義則, 信太 隆夫, 宮田 亮, 松山 隆治, 今村 光男, 伊藤 浩司
    原稿種別: 本文
    1969 年 18 巻 1 号 p. 56-69,90
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    1. Tree pollen season extended from the first week of April to the last week of June and significant trees were Birch, Alder, Oak, Spruce, Fir, Yew and Maple. Grass pollen season extended from the first week of June to the last week of August, but grass pollens were found until the middle of September. The most significant and abundant grasses were Orchard grass, Timothy, June grass, Rye grass and Red top, all of which were introduced. Weed pollens were found from the first week of June to the middle of September. Dominant weeds were Sheep sorrel, Blunt-leaved dock and Wormwood (Artemisia montana), but Short regweed, allergically troublesome plant, was not found anywhere in this area. The wormwood was found abundantly, showing a peak in the pollen counts at the begining of September in Sapporo area. 2. Variation in pollen incidence from year to year was wider in trees, particularly Birch, Alder and Maple, than in grasses. Counts of all pollen grains in 1 966 were recorded twice in 1965. Low pollen counts were obtained in the days when it was higher than 80% in relative humidity, and rainfall. Variation in pollen incidence from place to place was wide and high counts occured locally in the neighbouring area of Sapporo. Cyclic pollen releases in grasses were confirmed to particular times of day and the majority of the pollen was released in the early morning with mild wind. 3. The comparative study with the new pollen sampler (Durhams' standard sampler) and the old type of instrument (the pollen slide shelter employed in 1965 and 1966) during the grass season of 1967 revealed that higher counts were obtained with the old type than the new. 4. Vegetation of Hokkaido and Sapporo were described briefly from the point of view of allegy. Finally, the implication of this study were discussed.
  • 山本 昇壮
    原稿種別: 本文
    1969 年 18 巻 1 号 p. 70-80,90-91
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Histamine may be released from tissues by a number of agents. Among those, one of the most potent substance is Compound 48/80, a condensation product of ρ-methoxyphenylethyl methylamine with formaldehyde. Compound 48/80 is especially effective in producing mast cell degranulation and histamine release in various species, in vivo and in vitro, persumably through an enzymatic energy-requiring metabolic process. This experiment has been undertaken on the assumption that Compound 48/80 with these activities may have some effects on complement which has properties of enzyme. It has been found that a small amount of Compound 48/80 inhibit hemolytic activity of guinea-pig complement. The mechanism of the anticomplementary activity of Compound 48/80 has been investigated by its effect on the reaction of each component of guinea-pig complement mixed with Compound 48/80 to sensitized sheep erythrocyte-complement intermediates. Compound 48/80 has been found to inhibit selectively interaction of C'4 with EAC'1 cells. On the other hand, the interaction of guinea-pig or human C'1 with EAC'4 cells has been enhanced by Compound 48/80. It is interested that Compound 48/80, histamine liberater, has enhanced C' 1 activity. Evans Blue also has been found to inhibit hemolytic activity of complement and both of anticomplementary activity have disappeared by mixing of Compound 48/80 and Evans Blue together. From these results, it is suggested that Compound 48/80 and Evans Blue do not dinature components of complement irreversibly. Compound 48/80 has most inhibited passive cutaneous anaphylaxis (PCA) of guinea-pig produced with rabbit antibody at combined injection of antibody with Compound 48/80 intradermally.
  • 近藤 忠徳
    原稿種別: 本文
    1969 年 18 巻 1 号 p. 81-85,91
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Lentinus edodes (Berk) Sing is the most widely used edible mushroom in Japan. It is artificially cultivated on the wood planted the mushroom mycelium in a frame made from vinyl. A man aged 33, farmer, has started the mushroom cultivation since 1957. Seven years after he had had a first attack of dyspnea with wheezing subsequent to sneezing and stuffing nose during his labor in the frame. Since then, his attack has repeated almost always by the labor in the frame. It developed mostly within several hours after starting the job. A number of allergic intracutane tests on the patient revealed positive reaction to the extract from spore of the mushroom, and also to the candida, alternaria, house dust and Jukusan-Nyo, urine from matured silk worm larva. Eye tests as well as Prausnitz-Kustner tests, however, only positively reacted with the spore of the mushroom, but not with other above-mentioned allergens. Provocative inhalation tests also indicated that the spore was sole offending allergen to the patient. His chest X-ray revealed no abnormality. Hyposensitization therapy using the spore extract obtained excellent result so far. These findings indicate that the kind of bronchial asthma is obviously different from "Mushroom worker's Lung" proposed by A. Sakula (1967), but a new kind of allergic asthma so far as I know.
  • 原稿種別: 文献目録等
    1969 年 18 巻 1 号 p. 86-91
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原稿種別: 付録等
    1969 年 18 巻 1 号 p. 92-94
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原稿種別: 付録等
    1969 年 18 巻 1 号 p. 95-96
    発行日: 1969/01/30
    公開日: 2017/02/10
    ジャーナル フリー
feedback
Top