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Online ISSN : 1347-7935
Print ISSN : 0021-4884
ISSN-L : 0021-4884
17 巻, 1 号
選択された号の論文の12件中1~12を表示しています
  • 原稿種別: 表紙
    1968 年 17 巻 1 号 p. Cover1-
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原稿種別: 付録等
    1968 年 17 巻 1 号 p. App1-
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 小島 良平
    原稿種別: 本文
    1968 年 17 巻 1 号 p. 1-5,79
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    1) It has been established that the shock-toxisity upon mice, erythrogenic toxisity upon guinea pigs and haemolytic activity upon rabbit and horse red blood cells of staphylococcal α-toxin can inhibit from Cu-Chlorophillin. 2) Localised and generalised shock is not so specitic and might be a phenomenon of non-specific character that can inhibit from Cu-Chlorophillin in the same wise.
  • 鳥居 新平
    原稿種別: 本文
    1968 年 17 巻 1 号 p. 6-18,79
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    The purpose of the present study is to examine asthma attack in childhood chiefly by serological analysis of provoked asthma. 1) Twenty one of 42 asthmatic children developed clinical asthma by inhalational challenge of house dust antigen. 2) The group of the patients whose sera contained skin-sensitizing antibodies of high titers had significantly more who reacted to the provocation than the other whose sera contained those of low titers. 3) In those whose sera contained skin-sensitizing antibodies of low titers, seasonal variation in the positive rate of the provocation test was remarkably observed. 4) There seemed to be no consistent relationship between the hemagglutination titers and the skinsensitizing titers or, the times of hyposensitizations or the positive rate of the provocation. 5) The decreases of the skin-sensitizing titers were observed in the group of the patients who were challenged by the inhalation of house dust antigen but they were not observed in control group and hemagglutination titers showed no significant change. 6) Ten patients who did not developed asthma only by the provocation of house dust antigen and whose sera contained low skin-sensitizing titers were inhalationally challenged by house dust about 20 minutes after the injection of mecholyl chloride and 6 of them developed asthma. 7) The polygram patterns of the patients who developed asthma by the inhalational challenge of house dust antigen were similar to the patterns of those who developed asthmatic-like attack by the injection of mecholyl chloride and it was supposed that both patterns represented a state of sympathetic tunning.
  • 谷本 潔昭, 吉田 彪
    原稿種別: 本文
    1968 年 17 巻 1 号 p. 19-28,80
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    It is well known that serum complement level decreases markedly in anaphylactic shock. However, the role of complement is not so clear in delayed-type hypersensitivity. Therefore, serum complement activities were measured before and after desensitization to analyze the relationship between complement and delayed-type hypersensitivity. Materials and Methods: Guinea pigs were sensitized by an intramuscular injection of liquid paraffin emulsion containing 0.5 mg of heat killed tubercle bacilli (Aoyama B strain). Each of these animals showed a strong skin reaction to 0.1 ml of 2000 fold diluted old tuberculin (OT), i.e., more than 12 × 12 mm in redness and induration at 24 hours, when tested 8 weeks after the sensitization. These guinea pigs were divided into three groups. The first group was desensitized by an intravenous injection of PPD (Purified Protein Derivatives, 200 γ-4 mg). The second group was desensitized by an intraperitoneal injection of OT (1 mg of 10 fold diluted solutions to 1 ml of OT). The third group was desensitized by long term, gradually increased subcutaneous injections of OT. The guinea pig sera were taken before sensitization, just before, 1 hour after, 24 hours after and 48 hours after desensitization respectively. Following examinations were performed in each animal. 1. Fifty per cent hemolytic activity of complement (C'H50). 2. Fifty per cent immuno-adherence activity of complement (C'IA50). 3. Hemolytic antibody titer of Middlebrook-Dubos Reaction (M.D. titer). Results: 1. There was no significant difference in C'H50 between before and after sensitization. 2. Only slight reductions in C'H50 were observed in the guinea pigs desensitized by PPD. 3. Extremely marked reductions of C'H50 were noticed in the OT-desensitized group in accordance with the decrease of MD titer. 4. C'H50 almost recovered in 48 hours after desensitization. 5. C'H50 showed no significant changes in the gradually desensitized group. 6. C'IA50 changed well parallel with C'H50. Conclusions: The decrease in C'H50 was marked in the OT-densensitized animals in accordance with M.D. titers. While the changes were very slight in the PPD-desensitized. Therefore, the direct evidence of the significant role of serum complement was not obtained in delayed-type hypersensitivity.
  • 瀬戸 昭
    原稿種別: 本文
    1968 年 17 巻 1 号 p. 29-38,81
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Haptens are defined as substances which react specifically with antibody, but cannot provoke allergic reactions without being attached to a macromolecular carrier by covalent bonds, nor carrying two or more identical antigenic determinants. Having examined the biological activity of glycine ester, aspirin and their derivatives by PCA and Schultz-Dale reaction, it was confirmed that PCA was obtained using some univalent haptens containing only a single haptenic group, and that these haptens acquired this ability by reversible binding to serum albumin. Using as antigen a mixture of the dihaptenic substance containing two different determinant groups and the specific antibody globulin to one determinant, positive Schultz-Dale reaction was obtained on the intestinal strips sensitized with the specific antibody to another determinant. It was found that these haptens were incapable of desensitizing the sensitized animals, nor provoking anamnestic response.
  • 青木 良雄, Stanley Marcus
    原稿種別: 本文
    1968 年 17 巻 1 号 p. 39-47,81
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    The active principle responsible for delayed type of skin reaction in mycotic infections has been thought to be a polysaccharide. However, preparations of immunologically active polysaccharides derived from culture filtrates or organisms were not homogeneous and always contained significant amounts of nitrogen. In this study, therefore, the chemical property of skin test antigens isolated from coccidioidin and histoplasmin has been reexamined carefully by several methods. TCA precipitates and deproteinized ethanol precipitates obtained from coccidioidin contained 12.53% N and 6.13% N, respectively. Both fractions were skin test active. Pronase or trypsin trestment of both fractions markedly reduced their delayed skin test activities in infected guinea pigs. Whereas 0.1γ of the original polysaccharide fraction was equivalent to the standard dose, 1,000γ of the pronase treated"polysaccharide"was required to give a skin reaction of approximately same size. Furtheemore, skin test activity of histoplasmin and histoplasmin polysaccharide was also reduced by the treatment with pronase. When coccidioidin polysaccharide was injected with complete Freund's adjuvant into foot pads of guinea pigs, this fraction was capable of sensitizing animals for delayed skin reaction, but the pronase treated polysaccharide failed to sensitize animals. Although a homogeneous skin test active substance has not been isolated in this work, these results strongly suggest that the active principle of coccidioidin and histoplasmin is protein.
  • 青木 良雄, 仲吉 洋, 小野 美穂子
    原稿種別: 本文
    1968 年 17 巻 1 号 p. 48-55,82
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    In the previous paper, it was reported that the active principles for delayed skin reaction of coccidioidin and histoplasmin were considered to be a protein in nature. In this paper, it was attempted to define the chemical property of immunologically active substances of Candida. Organisms of C. albicans, Ca 13 strain, were repeatedly extracted with 0.1 N KOH solution and then with 1 N KOH solution. Precipitates were obtained from these extracts by HC1 precipitation at pH 4.0. Supernates were deproteinized with TCA and precipitated with ethanol. These fractions were skin test active and their activities were markedly reduced by the treatment with proteolytic enzymes. The crude protein fraction was further purified by zone electrophoresis and two purified polysaccharide fractions (D_3-1 and PS-1) were obtained by DEAE-cellulose column chromatography or by gel filtration with Sephadex G 200. The purified protein fraction contained 14.12% N and still 2.7% sugar. The purified polysaccharide fractions, D_3-1 and PS-1, contained 0.66% N and 97.2% suger, and 0.77% N and 95.3% sugar, respectively. PS-1 was immunologically homogeneous in agar gel precipitation and showed a symmetric peak in ultracentrifugal analysis (6.1 S). The skin test activity of the purified protein fraction was more than 100 times that of the purified polysaccharide fraction. When the purified protein or the crude polysaccharide fraction was injected with complete Freund's adjuvant into foot pads of guinea pigs, these fractions were capable of sensitizing animals for delayed skin reaction. However, the purified polysaccharide and the papain treated crude antigens failed to sensitize animals. These results indicated that the active principle of skin test antigen in Candida was protein, too.
  • 青木 良雄, 仲吉 洋, 小野 美穂子
    原稿種別: 本文
    1968 年 17 巻 1 号 p. 56-61,82-83
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Skin test activities of antigens separated from organisms of Sporotrichum Schenckii and Cryptococcus neoformans by French press extraction and of and antigen obtained from Aspergillus fumigatus by phenol extraction were reduced by the treatment with proteolytic enzyme, papain or pronase. From the results obtained in this and in two previous papers, it was concluded that the active principle of skin test antigen in mycotic infections was protein. On the other hand, the main component responsible for precipitation reaction was polysaccharide. In order to determine the chemical property of active component for precipitation reaction found in antigens from 6 species of fungi, sugar components in antigen-antibody complexes were analyzed by gas liquid chromatography. Only mannose was detected in Ag-Ab complexes of Candida antigen and histoplasmin, while the Candida and histoplasmin polysaccharide contained mannose and a small amount of glucose. In the same manner, xylose and mannose were detected in Cryptococcus Ag-Ab complex, rhamnose and mannose in Sporotrichum and galactose and mannose in Aspergillus, respectively. These antigens also contained a small amount of glucose, but no glucose was detected in these Ag-Ab complexes. From these results, it was concluded that the active component for precipitation reaction was mannan in Candida and histoplasmin polysaccharide, xylomannan in Cryptococcus, rhamnomannan in Sporotrichum and galactomannan in Aspergillus, respectively.
  • 松村 則幸
    原稿種別: 本文
    1968 年 17 巻 1 号 p. 62-78,83
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Ocular lesion is one of the frequent findings (over 80%) of the Behcet's syndrome and recently plays an important role in the causes of blindness in Japan. But the digestive tract is the most frequently affected site (98%) and oral aphtha is the most frequent initial symptom (58%). Intestinal abnormalities are proved by X-ray investigation in about 80%. Ocular lesion develops mostly later. Therefore, in view of the clinical features and laboratory findings in Behcet's syndrome experiments were designed using homogenate of the intestinal mucosa and Esherichia coli emulsified in incomplete Freund's adjuvant (Drakeol and Arlacel A) to sensitize rabbits once every week prolongedly. Rabbits of control group were sensitized either with E-coli and incomplete Freund's adjuvant or with homogenate of the intestinal mucosa and incomplete Freund's adjuvant. In 2-9 months 7 of the 10 rabbits showed disorders such as protrusion of the eye and opacity of the cornea. Only one of the 12 control group rabbits, sensitized by E-coli with incomplete Freund's adjuvant, showed a similar but very slight eye protrusion. Histologically round cell infiltration of the ciliary body, a marked perivascular edema and slight infiltration of the optic nerve were revealed. The hyaline change and amyloid deposition were proved in the glomeruli of these rabbits. Further, pericholangiolitic cell infiltration in the liver and slight submucosal cell infiltration in the intestine were observed. Inerstitial pneumonitis was observed but also in the control group. FITC labeled anti-rabbit-gammaglobulin-goat serum showed presence of bound gamma globulin at these lesions. Serologically rheumatoid factor like substance became positive in some sera of the rabbits immunized by E-coli with or without homogenate of intestinal mucosa. No positive results were proved by intracutaneous test on the above rabbits and by agar gel precipitation test (Ouchterlony technique) with the sera of the rabbits using extract of the intestinal mucosa as antigen. With tanned cell hemagglutination technique (sensitization with E-coli extract) a significant rise of anti E-coli antibody titer was proved in the sera of Behcet's disease patients. But intracutaneous reaction with coli-extract and Schwartzman filtrate of E-coli culture showed not significant change in Behcet's disease. Behcet's syndrome in man has no marked kidney change as shown in the above experiment. But the above mentioned result suggests a possible role of the normal or usual intestinal flora and digestive tract change in the development of the multiple manifestation of Behcet's disease.
  • 原稿種別: 文献目録等
    1968 年 17 巻 1 号 p. 79-83
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原稿種別: 付録等
    1968 年 17 巻 1 号 p. App2-
    発行日: 1968/01/30
    公開日: 2017/02/10
    ジャーナル フリー
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