VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 17

Displaying 151-200 of 318 articles from this issue

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STUDIES ON THIAMINASE I : (III) ON SOME PROPERTIES OF THE PURIFIED THIAMINASE I

Shinpei YOKOTA

Article type: Article
1959Volume 17 Pages 287-292
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_287

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As shown in the previous reports, a highly purified preparation of thiaminase I of Bacillus thiaminolyticus was obtained. In this paper some properties of the purified thiaminase preparation are reported. The thiamine-decomposing reaction by the purified thiaminase I showed first order kinetics. The optimal temperature was 50℃, and the optimal pH was 6.0 when pyridine was added to the reaction system. The enzymic activity was increased by Mn^<..>, Mg^<..> or Ca^<..>, etc., and inhibited by Cu^<..>, Ni^<..> or Zn^<..>, etc. It was activated by some chelating reagents, such as citrate, urea or histidine, and inhibited by some others, such as EDTA or NaCN. Furthermore, the addition of such an inorganic substance as sodium sulfide or sodium thiosulfate highly increased the enzymic activity and also its stability.

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STUDIES ON A NEW SYNTHETIC MEDIUM FOR BACILLUS THIAMINOLYTICUS : (I) ON THE COMPOSITION OF A NEW SYNTHETIC MEDIUM

Eiichi SAKAKIBARA, Shinpei YOKOTA, Masuyuki KATSUMATA, Toshikazu SEGUC ...

Article type: Article
1959Volume 17 Pages 293-298
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_293

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This report relates to a new synthetic medium for B.thiaminolyticus. The improved Kimura's C medium for B.aneurinolyticus by Sakakibara and Katsumata was used as the basal medium and studies on S-, N-, C-, P-source and other growth factors were carried out in detail. For the growth and enzyme production of B.thiaminolyticus, thiamine was required as S-source and glutamic acid as N-source, but glycerol or glucose being able to be utilized as C-source was not useful because of acidifying the synthetic medium. On the contrary, glutamic acid was used not only as a N-source, but as C-source. Thus the composition of a new synthetic medium for B.thiaminolyticus was as follows : glutaminate 20g, asparagine 5g, citrate 5g, NaCl 1g, KH_2PO_4 1g, (NH_4)_2HPO_4 5g, MgCl_2・6H_2O 0.7g, FeCl_2・6H_2O 0.03g, thiamine 500γ, water 1,000ml.

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STUDIES ON PANTOTHENIC ACID REQUIRING MUTANTS OF ESCHERICHIA COLI : (II) GROWTH REQUIREMENTS OF A β-ALANINE REQUIRING MUTANT

Tsuiko MANO

Article type: Article
1959Volume 17 Pages 299-305
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_299

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In the synthetic medium of Davis-Mingioli, the mutant strain #79,derived from E.coli O-20,responded to a much smaller amount of pantothenic acid than to the amount of β-alanine. The growth by β-alanine was inhibited by casein hydrolysate or certain amino acids. The growth activity of L-carnosine for the mutant was comparable to that of pantothenic acid. Water extract of beef supported the growth of the mutant even when diluted to 1 : 40,000. The activity was also found in the chicken extract. The experimental evidence is presented to show that the factor in meat extract responsible for the mutant may be carnosine, anserine or an analogous substance.

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INTRACELLULAR DISTRIBUTION OF RIBOFLAVIN-CONTAINING ENZYMES IN LIVER OF ALLOXAN DIABETIC RATS : (I) DISTRIBUTION OF FAD, FMN AND RIBOFLAVIN-PHOSPHORYLATING ACTIVITY IN LIVER CELLS

Yoshikata SUGIURA

Article type: Article
1959Volume 17 Pages 306-310
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_306

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The distribution of riboflavin in liver cell fraction (nuclear, mitochondrial, microsomal and supernatant) of alloxan diabetic rats decreased and the most significiant fall of FAD was found in the mitochondrial fraction. FAD in mitochondrial fraction was about 55 per cent of total FAD, and the remainder was almost equally distributed in the other fractions, whereas FMN distributed almost equally in all fractions. The phosphorylation of riboflavin took place in mitochondrial, microsomal and supernatant fractions. The phosphorylating ability was lower in alloxan diabetic liver than in the normal one.

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 310-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_310_1

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 310-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_310_2

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 310-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_310_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1959Volume 17 Pages 311-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_311_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1959Volume 17 Pages 311-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_311_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1959Volume 17 Pages 311-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_311_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1959Volume 17 Pages 311-312
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_311_4

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1959Volume 17 Pages 312-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_312_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1959Volume 17 Pages 312-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_312_2

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 312-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_312_3

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 312-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_312_4

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 313-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_313_1

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 313-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_313_2

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 313-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_313_3

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 313-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_313_4

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 314-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_314_1

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 314-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_314_2

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 314-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_314_3

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 314-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_314_4

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 314-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_314_5

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 315-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_315_1

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 315-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_315_2

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 315-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_315_3

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[title in Japanese]

Article type: Appendix
1959Volume 17 Pages 315-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_315_4

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 316-317
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_316

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 317-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_317_1

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 317-318
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_317_2

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 318-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_318_1

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 318-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_318_2

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 318-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_318_3

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 318-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_318_4

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STUDIES ON MYOCARDIAL METABOLISM : (I) INFLUENCE OF α-LIPOIC ACID ON MYOCARDIAL METABOLISM OF DOGS WITH EXPERIMENTAL CORONARY INSUFFICIENCY

Tomoe ITO

Article type: Article
1959Volume 17 Pages 319-330
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_319

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With the purpose to study the mechanism of the action of α-lipoic acid in the coronary insufficiency, experiments were made on 25 dogs which had been brought to coronary insufficiency. By the technique of coronary sinus catheterization, the myocardial metabolism was investigated with regard to both organic acids and electrolytes. After the operation of coronary insufficiency, the uptake ratios of pyruvate, lactate, α-ketoglutarate and inorganic phosphate decreased, but they were found to increase after the injection of α-lipoic acid. The coronary arteriovenous difference in the concentration of K and Mg fell temporarily after the operation up to negative values, but it was restored by an injection of lipoic acid. This effect of α-lipoic acid lasted for about 7-28 minutes, when the uptake of pyruvate and α-ketoglutarate was remarkably increased. From the above results, it is considered that α-lipoic acid improves the myocardial metabolism by a rapid activation of TCA-cycle in the effect of promoted oxidation of α-keto acids and therefore may have an effective influence on the coronary insufficiency.

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STUDIES ON MYOCARDIAL METABOLISM : (II) INFLUENCE OF ADENOSINE TRIPHOSPHATE ON THE MYOCARDIAL ELECTROLYTES METABOLISM IN EXPERIMENTAL CORONARY INSUFFICIENCY

Tomoe ITO

Article type: Article
1959Volume 17 Pages 331-339
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_331

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As a means to reveal the influence of ATP on the heart, ATP was injected intravenously to dogs with experimental coronary insufficiency, and the change of myocardial electrolyte metabolism was studied. Immediately or three minutes after the injection of ATP (20mg) the difference of Mg level between artery and vein showed an increase at first, that of K a remarkable increase, secondly, and then they rapidly decreased temporaly, which, however followed by an increase in various extent. Administration of ATP with cocarboxylase or α-lipoic acid showed a marked increase of the defference of K- and Mg-level in artery and vein within three minutes and 7-21 minutes after the injection. With regards to those of Na- and Ca-level, a definite trend were not found though in some cases it showed a reversal tendency to those of K- and Mg-level.

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 339-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_339_1

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 339-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_339_2

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 339-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_339_3

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 339-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_339_4

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EFFECT OF SEVERAL SUBSTANCES ON BACTERIAL THIAMINASE I

Masatoshi IKEDA

Article type: Article
1959Volume 17 Pages 340-343
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_340

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The effects of several heavy metals, amines, SH-compounds and other substances upon the thiaminases obtained from the culture broth or the bacterial cells of Bacillus thiaminolyticus and Clostridium thiaminolyticum. The results obtained indicated that the intracellular enzymes were not enhanced by some kinds of metals, but by certain amines. All substances examined had the same enhancing or inhibitory effect, on both enzymes, except Fe^<..> and Cr^<..>. Enhancement by aniline, homosulfamine, pyridine, nicotinamide and cysteine was shown to a low degree. The effects of such substances on the two differnt enzymes were essentially similar, while the thiaminase of Cl. thiaminolyticum was found remarkably different from that of shell-fisches or fishes.

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SOME PROPERTIES OF THIAMINASE I

Masatoshi IKEDA

Article type: Article
1959Volume 17 Pages 343-346
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_343

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The optimum pH of thiaminase obtained from the culture broth or the bacterial cell of Bacillus thiaminolyticus was 6.0 and that of Clostridium thiaminolyticus was 7.5. The decomposition products of thiamine by these two intracellular enzymes were shown to be identical. When the culture of B.thiaminolyticus grew older on the agar plate, the enzymatic activity decreased in proportion to the decrease of vegetative cells of the bacteria.

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 346-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_346_1

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 346-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_346_2

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 346-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_346_3

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HISTOCHEMICAL STUDIES ON FLAVONOIDS

Tsaiyin WANG

Article type: Article
1959Volume 17 Pages 347-364
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_347

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For histochemical observation of the flavonoids a new method using a fluorescence microscope was carried out by the application of an alkali-fluorescence method. The procedures of the method were described. With this method, the histological distribution pictures of flavonoids in each organ of rats and rabbits were observed. The animals were orally or subcutaneously given by hesperidin or rutin in normal condition or in the condition of blockade of reticuloendotherial system with indian ink or of the CCl_4 toxicosis. From these histological pictures, absorption and excretion of the flavonoids and the mode of action of the substances in living bodies was discussed.

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 364-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_364_1

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 364-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_364_2

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[title in Japanese]

[in Japanese]

Article type: Article
1959Volume 17 Pages 364-
Published: 1959
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.17.0_364_3

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