-
Kenzo OYA
Article type: Article
1959Volume 18 Pages
637-641
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Studies were made on emigration of vitamin B_2 (free riboflavin, FMN and FAD) into saliva. Saliva was taken from the salivary fistula made artificially at the submaxillary and sublingual glands of a healthy dog. Subcutaneus, intramuscular or intravenous injection of 1-20mg of free riboflavin or intravenous administration of 1-20mg of FMN caused no change in salivary vitamin B_2 within 3 hours at least. However, a small amount of FMN was found in saliva when 4mg of the substance had been injected intravenously. Injection of 10-20mg of FMN caused a remarkable emigration of vitamin B_2 into saliva. The most distinct result was observed in the case of FAD administration. Namely, the vitamin proved to be emigrated into saliva even when only 1mg had been injected. Paper chromatographical studies showed that the vitamin appeared in saliva in the esterified forms.
View full abstract
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
641-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
641-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
641-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
641-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
Akira HASHIMOTO
Article type: Article
1959Volume 18 Pages
642-647
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
From the cells of Mycobacterium avium, partially inhibited by sulfathiazole, 4-amino-5-imidazole carboxamide ribotide and a derivative of 4-amino-5-imidazole carboxamide riboside were separated. They were purified by using charcoal adsorption and IRA-400 ion-exchange chromatography. Properties of the derivatives were described in detail.
View full abstract
-
Akira HASHIMOTO
Article type: Article
1959Volume 18 Pages
648-650
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
4-Amino-5-imidazole carboxamide ribotide and 4-amino-5-imidazole carboxamide riboside derivative (tentatively named as D_1 substance), which had been separated from the cells of Mycobacterium avium partially inhibited by sulfathiazole, were studied on the influence on the biosynthesis of riboflavin. They were found to promote the flavin biosynthesis in vitro in the extract of acetone-dried normal cells of Mycobacterium avium. Their promoting actions were supported by coexistence of ATP and boiled extract of normal cells, and were degradedby cotransformylase.
View full abstract
-
Article type: Article
1959Volume 18 Pages
650-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
Chikataro KAWASAKI, Takatomo HORIO, Takako ASANO
Article type: Article
1959Volume 18 Pages
651-655
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
When 50 mg of Desoxymethyl-thiothiamine (I) was given subcutaneously to rats, it was easily decomposed into several metabolites and only 4.3% of the given (I) was excreted in the 24 hour-urine. The urine contained at least three fluorescent substances which showed Rf 0.33,0.42 and 0.53 on paper chromatography (butanol・acetic acid・water=4 : 1 : 5). After its adsorption on the column of ion-exchange resin IRC-50,successive eluates were devided into 3 fractions accordingly to Rf values and two kinds of crystals of mps. (decomp) 260℃ and 238℃ representing Rf 0.42 and 0.53,were isolated. The latter coincided with desoxymethyl-thiochrome (II) and the former would be the further metabolite of (II).
View full abstract
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
655-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
655-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
Chikataro KAWASAKI, Takatomo HORIO
Article type: Article
1959Volume 18 Pages
656-661
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Thiamine is unstable in its dilute solution with so-called thermostable factors such as rutin, hydroquinone etc. PABA also accelerates the oxidation of thiamine in its neutral or alkaline solution by dissolved oxygen but the exclusion of oxygen by saturation with N_2 or addition of alkaline ammonium ferrous sulfate and sodium tartrate prevents the oxidation. The PABA solution, irradiated by ultraviolet ray, was more effective to oxidize thiamine than the non-irradiated one, but its oxidation was greatly diminished by expelling dissolved oxygen by the same procedure. Dissolved oxygen carries out the oxidation of thiamine into thiamine disulfide in its alkaline solution of pH 9.5 most effectively, and the thermostable factors enable this oxidation even in less alkaline media. If dissolved oxygen is expelled, thiamine is stable in the alkaline medium even at the presence of thermostable factors. This evidence was presented by using rutin, hydroquinone, and extract from azelea leaves, thus thermostable factors destroying thiamine were proved as accelerators for the oxidation of thiamine by dissolved oxygen.
View full abstract
-
Tadayoshi ONO
Article type: Article
1959Volume 18 Pages
661-662
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Twenty-two strains of thiamine-degrading bacteria were isolated by enrichment method from the feces of 48 cattles ; 12 strains of them found to be Clostridium thiaminolyticum Kimura et Liao, 5 strains Bacillus thiaminolyticus Matsukawa et Misawa and 5 strains Bacillus aneurinolyticus Kimura et Aoyama.
View full abstract
-
Article type: Appendix
1959Volume 18 Pages
663-666
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
Masuyuki KATSUMATA, Shinpei YOKOTA, Kazunari HAGIHARA, Toshikazu SEGUC ...
Article type: Article
1959Volume 18 Pages
667-671
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
The resperatory metabolism of B. aneurinolyticus was remarkably promoted by the addition of the culture filtrate, as mentioned previously. It was concluded that the phenomenon owed to unknown factors which were contained in the culture filtrate. In this paper, the purification of these factors is reported. Phenol extraction from the culture filtrate saturated with ammonium sulfate and column chromatographical separation were successfully carried out. Then, two different substances were discovered. One of them was a respiration promoting factor and the other inhibitory one. Each of them had the absorption maximum at 260 mμ and 270 mμ respectively.
View full abstract
-
Masuyuki KATSUMATA, Kazunari HAGIHARA, Shinpei YOKOTA, Toshikazu SEGUC ...
Article type: Article
1959Volume 18 Pages
671-676
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
As previously reported, 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP) activated the glutamate metabolism of B. aneurinolyticus. The activation was characteristic in the glutamate metabolism, and such an effect was not exerted on the other amino acids. The effect of pyridoxine on RQ value of the oxidative degradation of glutamate was contradictory to that of OMP. These may be two different pathways of the glutamate metabolism, one of them is that through α-ketoglutarate and the other through pyrrolidone carboxylate.
View full abstract
-
Hisateru MITSUDA, Fumio KAWAI, Katsuharu YASUMATSU, Hiroshi ITO, Kazuo ...
Article type: Article
1959Volume 18 Pages
676-681
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Thiamine adhered on the foliage of rice plant was well absorbed in proportion to its concentration. The absorption rate was influenced by the pH of medium and was different with the forms of thiamine derivatives. In parallel with increase in the free form of thiamine, the leaves became also rich in the ester form. Over 5 per cent increase in the crop of grain was observed by the foliar application of thiamine in a range of 0.3 to 6 mg per stubble in the fields, and 10 per cent increase was als observed when the plant was grown in the nutrient solution containing 0.01 μg of thiamine per ml.
View full abstract
-
Kazuo MASUKO
Article type: Article
1959Volume 18 Pages
681-691
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
The effect of X-ray irradiation on thiamine metabolism and its mechanism were studied on albino rats and mice. The irradiation of 800 roentgens on the whole body of a white rat caused after 24 hours, a decrease of the thiamine content in each organ and increases in the blood and urine. For studying the mechanism, thiamine load experiments and in vitro experiments using mitochondria were made. An inhibition of thiamine phosphorylation was found. In preventing a mouse from the X-rays effect, thiamine propyldisulfide was fairly active, and α-lipoic acid and thiamine diphosphate slightly active.
View full abstract
-
Harushige SAKAMOTO
Article type: Article
1959Volume 18 Pages
691-695
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
The metabolic fate of ampeloptin by Penicillium sp. has been studied. Gallic acid and phloroglucinol carboxylic acid was found in the broth. It was proved that not only the absorption of ampeloptin from the cell membrane occurred but also that cleavage of the middle ring of the flavonol took place, accompained by α-oxydation of side chain of phenylacetic acid derivative, thus formed, to yield gallic acid.
View full abstract
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
696-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
696-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
696-697
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
697-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1959Volume 18 Pages
697-698
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
698-699
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
699-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
699-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
699-700
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
700-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
700-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
700-701
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
701-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
701-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1959Volume 18 Pages
701-702
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
702-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
702-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
702-703
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
703-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
703-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
703-704
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
704-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
704-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
704-705
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
705-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
705-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
705-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1959Volume 18 Pages
705-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
706-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
706-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
706-707
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS