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[in Japanese]
Article type: Article
1959 Volume 18 Pages
373-
Published: 1959
Released on J-STAGE: December 22, 2017
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Shigeru MIYAZAWA
Article type: Article
1959 Volume 18 Pages
374-377
Published: 1959
Released on J-STAGE: December 22, 2017
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The effect of Aureomycin on the riboflavin assimilation of Streptococcus faecalis R was investigated. The addition of Aureomycin to the medium inhibited neither the permeation of riboflavin into the cell nor the riboflavin biosynthesis, but the conversion of riboflavin to its ester type was inhibited by Aureomycin. It is conceivable that Aureomycin does not combine with riboflavin or that the combined product is utilized by lactic bacteria.
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Shigeru MIYAZAWA
Article type: Article
1959 Volume 18 Pages
377-379
Published: 1959
Released on J-STAGE: December 22, 2017
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The stimulative effect of chloramphenicol on the folic acid metabolism of Lactobacillus arabinosus 17-5 was investigated. The permeation of folic acid into the cell was not stimulated, but was inhibited. The folic acid biosynthesis and the conversion of folic acid to folinic acid were also not stimulated. by the addition of chloramphenicol.
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Osamu TERADA, Kazuo OHISHI, Shukuo KINOSHITA
Article type: Article
1959 Volume 18 Pages
380-385
Published: 1959
Released on J-STAGE: December 22, 2017
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Chemical changes in the lactose-enriched media were investigated. Glucose was selectively utilized initially, then residual lactose was utilized after a fairly long adaptation period. A stational phase at about pH 8.0 was observed during lactose utilization. A marked increase of mycelial growth was observed in the initial stage of the lactose-utilizing phase. Comparatively rapid and durable formation of vitamin B_<12> was obtained by the lactose enrichment. An accelerated fermentation was resulted by forced aerations in jar fermentor. Some characteristic changes were observed in pH, mycelial growth and vitamin B_<12> liberation in the medium.
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Osamu TERADA, Kazuo OHISHI, Shukuo KINOSHITA
Article type: Article
1959 Volume 18 Pages
385-393
Published: 1959
Released on J-STAGE: December 22, 2017
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Lactose-utilizing mutants (D type) were isolated from Streptomyces olivaceus by a prolonged culture on the lactose-Bennett's agar. The D type could also be induced from the lactose-non-utilizing type (F type), but the reverse mutation could not be detected. The D type was found morphologically distinguishable from the original F type in giant colonies. The lag phase between glucose- and lactose-utilizing phases which was found previously with the original type on lactose-enriched media could not be found with the D type. A new morphological mutant (B type) with characteristic black reverse was isolated from an aged culture stock of Stm. olivaceus No. G.
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Shigeru ARAMAKI, Shozo NAKAMURA
Article type: Article
1959 Volume 18 Pages
393-396
Published: 1959
Released on J-STAGE: December 22, 2017
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In the previous paper, the author reported that ethylthiamine showed almost the same grade of growth promoting effect on rats as thiamine but the liver extracts of these animals showed only low cocarboxylase activity in spite of the fact the extracts contained relatively large amounts of so-called bound thiamine as determined by the thiochrome method. This was interpreated that ethylthiamine pyrophosphate was synthesized in the animal tissue and the pyrophosphate had a weak activity as cocarboxylase. In the present report, consequently, pure samples of ethylthiamine pyrophosphate were synthesized chemically and their cocarboxylase activity was measured manometrically. It was revealed that the synthetic samples had 1/12 to 1/15 of the activity of cocarboxylase.
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Shintaro KAMIYA
Article type: Article
1959 Volume 18 Pages
396-400
Published: 1959
Released on J-STAGE: December 22, 2017
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In the previous papers, it was reported that L-ascorbic acid decomposing enzymes of bacteria were produced adaptively only in the cells grown in a medium containing L-ascorbic acid, but not in the unadapted cells even when they were in contact with L-ascorbic acid for a long time. In this paper, it was shown that the unadapted cells became able to metabolize L-ascorbic acid when a small quantity of glucose and casein hydrolyzate were given. Both D-araboascorbic acid and D-glucoascorbic acid too were decomposed by L-ascorbic acid decomposing cells, but other reductones such as reductic acid, dihydroxymaleic acid, and 5-methyl-3,4-dihydroxytetrone were not. On the other hand, some properties of L-ascorbic acid oxidase found in the unadapted cells were studied. Its optimum pH was about 5.0 and the optimum concentration of the substrate was 800μM. It was found, too, that endiol compounds such as D-araboascorbic acid, reductic acid and 5-methyl-3,4-dihydroxytetrone, etc. were not decomposed by this enzyme.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
400-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
400-
Published: 1959
Released on J-STAGE: December 22, 2017
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Shintaro KAMIYA
Article type: Article
1959 Volume 18 Pages
401-406
Published: 1959
Released on J-STAGE: December 22, 2017
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In this paper it was presumed that L-ascorbic acid was metabolized by two different pathways. One of them might be carried through the course of D-araboascorbic acid and, perhaps its phosphate → 3-keto-6-phosphogluconate and equilibrium mixture of the two appeared to be formed. The latter was already known as an intermediate in the conversion of 6-phosphogluconate to ribulose-5-phosphate and D-ribose-5-phosphate. On the other hand, the presence of Warburg-Dickens pathway in the cells was supposed from the experiments that D-gluconic acid and D-ribose were metabolized by the cells while other pentoses such as DL-xylose, DL-arabinose were not. From the results above mentioned, one of the metabolic pathway of L-ascorbic acid was proposed as the following schemes : [chemical formula]
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Shintaro KAMIYA
Article type: Article
1959 Volume 18 Pages
407-411
Published: 1959
Released on J-STAGE: December 22, 2017
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In the previous paper, it was presumed that one of the pathways of L-ascorbic acid metabolism might be carried through dehydroascorbic acid and diketo-L-gluconic acid. In this paper this pathway was studied more precisely. Glyoxal, glyoxlic acid and mesoxalic acid were not decomposed by L-ascorbic acid decomposing cells. Both dehydroascorbic acid and D-arabinosone were metabolized not by unadapted cells, but by L-ascorbic acid adapted cells slowly, and the latter metabolic rate was acceralated when a small quantity of glucose or L-ascorbic acid was added as hydrogen donators. From the finding that the volume of O_2 uptake for DL-glyceraldehyde (15μM) by L-ascorbic acid adapted cells was about half that of D-glyceraldehyde (15μM), it was presumed that perhaps L-glyceraldehyde was not metabolized. From the results above mentioned, the course via dehydroascorbic acid was presumed as follows : L-Ascorbic acid→dehydroascorbic acid→diketo-L-gulonic acid→(L-xylosone)→D-arabinosone→D-ribose.
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Shintaro KAMIYA, Toshio NAKABAYASHI
Article type: Article
1959 Volume 18 Pages
411-417
Published: 1959
Released on J-STAGE: December 22, 2017
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In this paper, two different pathways of L-ascorbic acid metabolism were established as shown in Fig.6. One of them was carried through dehydroascorbic acid and corresponding diketo acid. It was first decarboxylated to L-xylosone to be converted to D-arabinosone by epimerization of C_4-OH group and then reduced to D-ribose prior to phosphorylating process. On the other pathway, L-ascorbic acid was first converted to D-araboascorbic acid by inversion of C_5-OH group, which was presumed to form equilibrium with 3-keto-D-gluconic acid. The latter was phosphorylated and was introduced into Warburg-Dicken's pathway. D-Ribose-5-phosphate, which occurred as common intermediate in two different pathways of L-ascorbic acid metabolism, was cleaved to yield active glycolaldehyde and 3-phosphoglyceraldehyde, as shown already by many reports. The former was oxidized to acetic acid, and the latter was metabolized through several steps to pyruvic acid. Pyruvic acid was, as reported in the previous paper, further metabolized to CO_2 and H_2O through TCA cycle, remaining a small quantity of acetic acid, D-lactic acid and formic acid.
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Tatsuya WATANABE
Article type: Article
1959 Volume 18 Pages
418-421
Published: 1959
Released on J-STAGE: December 22, 2017
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The final body weights of the newly weaned DDN- and DD-strain mice fed with ordinary mixed diet for 40 days were 29 and 26.5g respectively. No difference was found between the occidental and Oriental Co. types of diet. After feeding young mice for 18 days with synthetic diet without thiamine. The vitamin was added to the diet. Adding of 1μg resulted in no increase and 3-5μg a light increase in the body weight. Adding of 20-50μg of the vitamin showed a remarkable recovery of the body weight at the 40th day, catching up to the level of the mice fed with synthetic diet with 30μg of thiamine from the beginning. The mice grown up to about 20g showed remarkable decrease in body weight by giving the thiamine deficient diet for 18 days and could not recover or almost died even when they were given 100 μg per day of thiamine there-after.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
421-
Published: 1959
Released on J-STAGE: December 22, 2017
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Shizuo YAMADA
Article type: Article
1959 Volume 18 Pages
422-424
Published: 1959
Released on J-STAGE: December 22, 2017
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Dietary survey using albino rats demonstrated that riboflavin-5'-monosulfate was an antivitamin for riboflavin. When riboflavin-5'-monosulfate was injected, 500 μg of it was able to compete with 10 μg of riboflavin. When administered orally, however, the competition of riboflavin-5'-monosulfate was not observed by using the mixture of 500 μg of riboflavin-5'-monosulfate and 10 μg of riboflavin.
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Minoru HARADA, Shizuo YAMADA
Article type: Article
1959 Volume 18 Pages
425-426
Published: 1959
Released on J-STAGE: December 22, 2017
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Stability of FAD in aqueous solution saturated with ammonium sulfate was examined. In this solution, FAD was not decomposed to other flavin derivatives within the range of pH 5.0-8.0,standing at room temperature for 1-5 hours. Fourth flavin compound was also stable under the same conditions.
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Hirofumi YAGI
Article type: Article
1959 Volume 18 Pages
426-431
Published: 1959
Released on J-STAGE: December 22, 2017
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Flavin in the living tissue of atypical epithelioma was estimated to be 1.2γ/g composed of FAD (1.0 μg/g), FMN (0.16 μg/g), and riboflavin (0.04 μg/g), and that of its necrotic tissue was 0.8 μg/g composed of FAD (0.23 μg/g), FMN (0.53 μg/g) and riboflavin (0.04 μg/g). Total amounts of flavins in the organs of tumour bearing rat were decreased with the growth of tumour, reaching to be 18.0 μg/g in the liver, 22.6 μg/g in the kidney, and 10.3 μg/g in the heart at the end of the 4th week. The decrease of the total amount of flavin was attributed to the decrease of FAD. The administration of FAD elevated the amount of flavins in these organs, but the amount reached nearly the level of control after 5 hours. The administration of riboflavin-5'-monosulfate did not provoke any marked change in distribution of flavins in the organs of these rats.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
431-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
431-
Published: 1959
Released on J-STAGE: December 22, 2017
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Akira OGATA, Takashi MUKAI
Article type: Article
1959 Volume 18 Pages
432-437
Published: 1959
Released on J-STAGE: December 22, 2017
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The authors measured the thiamine and riboflavin concentrations in the maternal blood, chorion and fetus of healthy women in the early stage of pregnancy (2 to 3 months), and simultaneously observed the effects of oral administrations of thiamine hydrochloride and thiamine propyl disulfide (each totalled 75 mg for 5 days) upon the respective concentrations in these materials. The results are as follows : The total thiamine content in the maternal blood in the early stage of pregnancy was relatively low, indicating that many pregnant women were thiamine-deficient. Thiamine in the chorion was mostly in ester form, but that in the fetus was relatively rich in free form. The thiamine concentration in the chorion and fetus was increased by administrations of thiamine hydrochloride and thiamine propyl disulfide, but that in the maternal blood did so by thiamine propyl disulfide administration alone. The increase in the thiamine concentration in the chorion was greater in the administration of thiamine propyl disulfide than in thiamine hydrochloride. In the increasing state of the thiamine concentration in each material owing to thiamine hydrochloride and thiamine propyl disulfide administrations, a positive correlation was recognized only between the chorion and the fetus. The administrations of thiamine preparations did not have any effect on the riboflavin concentration in each material. Based upon the above-described findings, the authors assume that the administrations of thiamine hydrochloride and thiamine propyl disulfide are beneficial to the mother and fetus.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
437-
Published: 1959
Released on J-STAGE: December 22, 2017
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Akira OGATA
Article type: Article
1959 Volume 18 Pages
438-444
Published: 1959
Released on J-STAGE: December 22, 2017
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As it was found that the industrial patients poisoned with trichloroethylene showed thiamine deficiency, the author carried out experiments using animals to observe the relationship between the exposure to trichloroethylene and the thiamine metabolism. In animals exposed to trichloroethylene, the loss of body weight, decrease in the total thiamine concentration in blood, and acute and marked drop of urinary excretion of thiamine were recognized. In animals loaded with thiamine the rate of thiamine excretion was found to decrease. From these findings it was concluded that trichloroethylene exposure caused disturbances of thiamine metabolism. The gain of liver weight and pathological changes of the heart, liver, kidney and adrenal body were also observed.
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Akira OGATA
Article type: Article
1959 Volume 18 Pages
444-450
Published: 1959
Released on J-STAGE: December 22, 2017
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In the present experiment observing changes in the body weight and thiamine amount in blood and urine, and histological changes in the liver, it was concluded that the administration of thiamine-HCl, thiamine propyldisulfide and thiamine β-hydroxyethyl disulfide at trichloroethylene exposure acted effectively. Among these preparations, thiamine propyl disulfide was most effective.
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Akira OGATA
Article type: Article
1959 Volume 18 Pages
450-455
Published: 1959
Released on J-STAGE: December 22, 2017
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As a result of the examination on the disturbances of thiamine metabolism caused by trichloroethylene exposure, it was noticed that thiamine phosphate was excreted in the urine of rabbits and rats and sometimes of men. On exposure to trichloroethylene the thiamine in urine decreased but the thiamine phosphate hardly decreased or even developed a tendency to increase, so that the rate of thiamine phosphate excreted in urine increased. There may be a possibility of this phenomenon being utilized as a means of finding out the trichloroethylene poisoning. In rats it was perceived that the composition of feedstuff exerted an influence upon the thiamine phosphate amount excreted in the urine.
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Akira OGATA
Article type: Article
1959 Volume 18 Pages
455-457
Published: 1959
Released on J-STAGE: December 22, 2017
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To clarify the phenomenon that the excretion of thiamine phosphate in urine was promoted by trichloroethylene exposure, the author examined the activities of acid-, alkaline- and thiamine-pyrophosphatase in liver and kidney, and obtained the result that the activity of acid-phosphatase in liver and kidney was lowered by trichloroethylene exposure.
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Akira OGATA, Takashi MUKAI, Yasukiyo YONEOKA
Article type: Article
1959 Volume 18 Pages
457-459
Published: 1959
Released on J-STAGE: December 22, 2017
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The author observed the riboflavin level in blood and urinary riboflavin excretion in rabbits, loaded with riboflavin under the exposure to trichloroethylene. A slight decrease of riboflavin compared with normal animals was recognized.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
459-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
459-
Published: 1959
Released on J-STAGE: December 22, 2017
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Juichi OGATA
Article type: Article
1959 Volume 18 Pages
460-463
Published: 1959
Released on J-STAGE: December 22, 2017
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Evidence was presented to show that thiaminase of Bacillus aneurinolyticus actually hydrolyzes 2-methylthio analog of thiamine. The degradation products were identified by separating them by paper partition chromatography and measuring a ultraviolet absorption spectrum of the separated compounds. The hydrolysis of this compound was inhibited by thiamine pyrimidine more remarkably than thiamine was the case, and by thiamine thiazole which acted on thiamine only slightly. The hydrolysis of 2-methylthiothiamine was also inhibited by a high concentration of the pyrimidine moiety of this compound. 2-Methylthiothiamine was also decomposed by the staphylococcal extract. In this case, however, several pyrimidyl compounds revealed their inhibitory effects against the decomposition.
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Taro NAGASAWA, Tamotsu TANAHASHI
Article type: Article
1959 Volume 18 Pages
464-468
Published: 1959
Released on J-STAGE: December 22, 2017
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To study the reasons for the low thiamine content of milk in Japan and also the relation of latent mastitis to the content and partition of thiamine in the milk, we determined the contents of total thiamine and various forms of thiamine in the milks drawn from 28 mastitis negative quarters and from 24 mastitic quarters by the thiochrome method. The fractionation of thiamine was performed by a modified Houston's method. The content of total thiamine in apparently normal milk varies from 21.1-56.7 μg/dl, on an average 34.9±3.06μg/dl composed of 60.7% of free thiamine, 33.6% of cocarboxylase and 5.7% of protein-bound thiamine. In latent mastitis, the content of total thiamine was lower by 10 to 15 percent according to the degrees of infection.
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Yoshiaki TSUJI
Article type: Article
1959 Volume 18 Pages
468-476
Published: 1959
Released on J-STAGE: December 22, 2017
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Aureomycin, Chloromycetin and Achromycin were administered orally on normal children for 7 days, and thiamine level of blood, fecal and urinary excreation were determined daily. The urinary excreation of thiamine once increased and then decreased in the case of Aureomycin and Achromycin administration. On the other hand it was increased by Chloromycetin. The fecal excreation of thiamine remarkably decreased immediately after antibiotics administration. Disturbance of thiamine metabolism was observed after 7 days' administration of the autibiotics by thiamine loading test. On the contrary thiamine level of blood was kept almost constant during this period and thiamine deficient signs were not found clinically.
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Yoshiaki TSUJI
Article type: Article
1959 Volume 18 Pages
476-481
Published: 1959
Released on J-STAGE: December 22, 2017
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Thiamine levels of the hepatic venous blood and femoral arterial blood of a healthy dog were determined after an intramuscular injection of the vitamin, and it was confirmed that the intramuscularly administered thiamine was phosphorylated in liver and appeared in the hepatic vein 30 or 60 minutes after. Most of increased blood thiamine by administration of cocarboxylase was found to be esterified forms and a small part of it was hydrolyzed in the liver.
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Yoshiaki TSUJI
Article type: Article
1959 Volume 18 Pages
481-491
Published: 1959
Released on J-STAGE: December 22, 2017
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Daily administration of various antibiotics and homosulfamine was carried out for 6 days. Thiamine contents of blood, urine and feces were determined everyday, and then examination was made, using a liver catheter, on the behavior of administered thiamine. Intramuscular injection of streptomycin and oral administration of Aureomycin, Chloromycetin and homosulfamine caused disturbance of thiamine metabolism, especially of its phospholyration, and decrease of thiamine level of blood, while orally administered streptomycin had not such effects on the metabolism. However, fecal excretion of thiamine decreased remarkably in all cases. These results indicate that thiamine deficiency or its metabolic disturbance is not due to the inhibition of thiamine synthesis by intestinal bacteria, but to the action of antibiotics itself.
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Soichi NIWA, Nobu KATAYAMA, Shizuya MATSUBARA
Article type: Article
1959 Volume 18 Pages
492-497
Published: 1959
Released on J-STAGE: December 22, 2017
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To prevent the decomposition of vitamin C added to the soft drink for its enrichment, the following attentions were recommended ; (1) to mix polyphosphoric acid, (2) to remove Fe, Cu and other minerals from the water as much as possible, and (3) to store at the temperature less than 20℃.
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Junko EBARA, Kiku MURATA
Article type: Article
1959 Volume 18 Pages
497-501
Published: 1959
Released on J-STAGE: December 22, 2017
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The method of the determination of a base exchange ability of thiaminase I which exchanges thiazole in the molecule of thiamine with pyridine was investigated using crude or partially purified thiaminase I of Bacillus thiaminolyticus. It was recognized that the amounts (0-1.0 μmoles) of heteropyrithiamine, a pyridine exchange product of thiamine, and the extinction coefficient at 386 mμ were relative when heteropyrithiamine was oxidized with alkaline ferricyanide after destroying thiamine (0.75 μmoles supposed to be present in a reaction mixture) by alkali. It was also observed that concentration of the acetone preparation of thiaminase was related with amounts of heteropyrithiamine produced to 5 μmoles. The optimum conditions of the base exchange reaction of the acetone preparation were pH 6.5 and 30℃ when the above method was applied. The examples of the specific activities at each step of the purification were shown by this method.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
501-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
501-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
501-
Published: 1959
Released on J-STAGE: December 22, 2017
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Tatsuya NUKINA
Article type: Article
1959 Volume 18 Pages
502-507
Published: 1959
Released on J-STAGE: December 22, 2017
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The applicability of Farrand fluorophotometer for the determination of vitamin A was described. The vitamin A contents of liver, small intestine and blood serum were determined, and it was found that the results were similar in rats fed on standard diet. The average total vitamin A level in liver of rats was 56.6 or 9.02 μg/g and vitamin A ester was 52.9 or 8.43 μg/g. That of small intestine was 5.11 or 1.59 μg/g and that of blood serum was 29.0 μg%. The vitamin A contents of liver, small intestine and blood serum were determined one, three, six and twenty-four hours after an intravenous injection of 1 mg of vitamin A per kg of body weight to normal rats. The vitamin A content increased remarkably in the liver, and that of the liver and small intestine reached the maximum level after six hours, whereas that of blood serum decreased after the vitamin A injection.
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Tatsuya NUKIYA
Article type: Article
1959 Volume 18 Pages
507-512
Published: 1959
Released on J-STAGE: December 22, 2017
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The effects of tetracycline on the vitamin A contents of liver, small intestine and blood serum were studied in rats. By addition of 50 mg of tetracycline per kg of body weight to standard diet, vitamin A level of small intestine and blood serum was not significantly altered, but in the liver it was increased to one and a half times after four weeks feeding, and this was in a large part due to the ester form. The vitamin A contents of liver, small intestine and blood serum were determined in one, three, six and twenty four hours after an intravenous injection of vitamin A, 1 mg per kg of body weight, to the tetracycline administrated rats. The vitamin A level of small intestine indicated no significant variation. However, the vitamin A level of liver, reaching a maximum after three hours, rose more rapidly than that of the loading test group in normal rats. This result showed that vitamin A storage in liver was accelerated by tetracycline administration. The increase of vitamin A content in liver was probably due to the increased vitamin B_<12> by tetracycline administration, which made vitamin A conversion from carotene easier, thus resulting in added storage of vitamin A in liver.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
512-
Published: 1959
Released on J-STAGE: December 22, 2017
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Tatsuya NUKINA
Article type: Article
1959 Volume 18 Pages
513-518
Published: 1959
Released on J-STAGE: December 22, 2017
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The effect of penicillin on the vitamin A contents of liver, small intestine and blood serum in rats was studied. Penicillin was added to the diet at the rate of 20000 units per kg of body weight, and after one, two, three and four week feeding vitamin A level was determined. The vitamin A content of liver was gradually increased two times after four weeks feeding, whereas that of blood serum was decreased slightly two and three weeks feeding, but in four weeks it was restored to normal level, and vitamin A level in small intestine showed insignificant changes, as compared with control groups. The vitamin A content of liver, small intestine and blood serum was determined one, three, six and twenty four hours after an intravenous injection of 1 mg per kg of body weight of vitamin A to the rats reared on penicillin-containing diet. The result showed that the vitamin A content of liver reached the maximum after three hours and the liver storage of vitamin A was accelerated by feeding on penicillin containing diet.
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Tatsuya NUKINA
Article type: Article
1959 Volume 18 Pages
518-521
Published: 1959
Released on J-STAGE: December 22, 2017
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The vitamin A contents of liver, small intestine and blood serum were determined after one, two, three and four weeks feeding on chloramphenicol containing diet, and one, three, six and twenty four hours after an intravenous injection of 1 mg per kg of vitamin A to the chloramphenicol administered rats. Each experimental result showed no significant changes as compared with control groups.
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Tatsuya NUKINA
Article type: Article
1959 Volume 18 Pages
521-525
Published: 1959
Released on J-STAGE: December 22, 2017
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The vitamin A contents of liver, small intestine and blood serum were determined by the fluorometric method after one, two, three and four week feeding on 200 mg per kg of body weight of homosulfamine containing diet. No significant changes as compared with control groups were found in the vitamin A contents. The vitamin A contents of liver, small intestine and blood serum were determined one, three, six and twenty four hours after an intravenous injection of 1 mg of vitamin A to the rats fed four weeks on homosulfamine containing diet. The results showed that after vitamin A administration the vitamin content of liver slowly increased and that of blood serum slowly decreased while the vitamin A in small intestine underwent insignificant changes, as compared with the control groups. It could be postulated that the change of vitamin A in liver and blood serum after loading of the vitamin was due to liver dysfunctions by homosulfamine administration.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
525-
Published: 1959
Released on J-STAGE: December 22, 2017
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Sei TACHIBANA
Article type: Article
1959 Volume 18 Pages
526-527
Published: 1959
Released on J-STAGE: December 22, 2017
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It was found in the extract of Beta vulgaris that the transglycosidation with riboflavin was stimulated by light as much as 25 % of control.
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Sei TACHIBANA
Article type: Article
1959 Volume 18 Pages
527-530
Published: 1959
Released on J-STAGE: December 22, 2017
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The occurrence of photo-transglycosidation with riboflavin was found in plant, for the formations of riboflavinylglucoside and oligosaccharides from maltose and riboflavin by the extracts of cotyledons of Cucurbita pepo were so remarkably stimulated by light as twice of control in 8 hours at room temperature.
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
530-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
530-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959 Volume 18 Pages
530-
Published: 1959
Released on J-STAGE: December 22, 2017
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