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[in Japanese]
Article type: Article
1959Volume 18 Pages
530-
Published: 1959
Released on J-STAGE: December 22, 2017
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Kunio OKUDA
Article type: Article
1959Volume 18 Pages
531-538
Published: 1959
Released on J-STAGE: December 22, 2017
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In this article, the basic techniques of studying the growth of rats on experimental diets has been described with additional instructions on how to produce deficiencies in vitamins, such as vitamin B_<12> and pantothenic acid, and obesity, and on the use of metabolism cages for nutritional works.
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[in Japanese]
Article type: Article
1959Volume 18 Pages
538-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 18 Pages
538-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 18 Pages
538-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
539-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
539-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1959Volume 18 Pages
539-540
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1959Volume 18 Pages
540-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
540-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 18 Pages
540-541
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959Volume 18 Pages
541-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
541-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
541-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
542-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
542-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
542-543
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
543-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1959Volume 18 Pages
544-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
544-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 18 Pages
544-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
545-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 18 Pages
545-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 18 Pages
545-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 18 Pages
545-
Published: 1959
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1959Volume 18 Pages
546-
Published: 1959
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1959Volume 18 Pages
546-
Published: 1959
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1959Volume 18 Pages
546-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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Article type: Appendix
1959Volume 18 Pages
546-
Published: 1959
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1959Volume 18 Pages
547-
Published: 1959
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1959Volume 18 Pages
547-
Published: 1959
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1959Volume 18 Pages
547-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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Article type: Appendix
1959Volume 18 Pages
547-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959Volume 18 Pages
548-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959Volume 18 Pages
548-
Published: 1959
Released on J-STAGE: December 22, 2017
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Kunio YAGI
Article type: Article
1959Volume 18 Pages
549-557
Published: 1959
Released on J-STAGE: December 22, 2017
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Toshio FUKUSHI
Article type: Article
1959Volume 18 Pages
558-561
Published: 1959
Released on J-STAGE: December 22, 2017
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It was observed that the fluorescence activity of dibenzoyl thiamine (DBT) was identical with that of thiamine in molar equivalence in the thiochrome reaction with alkaline hydrolysis. The standard curve of thiamine converted from DBT curve coincided with the calibration curve of thiamine itself by the alkaline hydrolysis method. Thiamine could therefore be used as the standard solution for determining DBT, and a part of procedure of the alkaline hydrolysis method was modified. The total amount of vitamins was determined as thiamine unit for DBT, or DBT and thiamine mixture by this modified method, and the mean value of recovery was 99.7%.
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Akira OKAMURA
Article type: Article
1959Volume 18 Pages
561-574
Published: 1959
Released on J-STAGE: December 22, 2017
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Thiamine level in blood, especially its distribution in plasma and blood cells, was determined in various diseases. The total thiamine level in the blood of 20 healthy subjects averaged 8.2±0.4 μg/dl, and no significant difference was found between male and female. Free thiamine was 0.9±0.3 μg/dl in average and found only in plasma, while a majority of esterified thiamine averaged 7.3±0.4 μg/dl, and existed in blood cells. The thiamine level in whole blood was lower in various diseases than that of healthy subjects. Especially in beriberi, hepatocholecystocholedochopathy with jaundice, pyloric prolapse and antrospasm, or neuromyopathic diseases, the content of esterified thiamine in blood cells was less than 4.0-4.5 μg/dl, and the concentration was lower than 10 μg/dl. Based on the above findings, the author concluded that it was very important to determine not only the thiamine in whole blood, but also the esterified thiamine in blood cells in order to diagnose the deficiency of thiamine.
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[in Japanese]
Article type: Article
1959Volume 18 Pages
574-
Published: 1959
Released on J-STAGE: December 22, 2017
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Akira OKAMURA
Article type: Article
1959Volume 18 Pages
575-581
Published: 1959
Released on J-STAGE: December 22, 2017
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The author determined the content of thiamine in whole blood and its distribution in plasma and blood cells when administering thiamine hydrochloride, thiamine propyldisulfide and cocarboxylase, and obtained the following results : After administering the above thiamine preparations, the thiamine increases in plasma as a free form, and then shifts to blood cells after being phosphorylated in body. When administering the above preparations for a long time, the esterified thiamine is saturated first in blood cells (25 μg/dl in blood cells, while 10 μg/dl in whole blood), and then the total thiamine becomes saturated in whole blood. The free thiamine, however, hardly increases in blood cells. Based on the above findings, the author states that the determination of the esterified thiamine in blood cells is more adequate than that in whole blood as a criterion of the thiamine deficiency.
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Hiroji TAKANO
Article type: Article
1959Volume 18 Pages
581-591
Published: 1959
Released on J-STAGE: December 22, 2017
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Biochemical variations in PABA requiring mutants were studied. Three mutants were derived from an E. coli 0-20 standard strain and were stocked in a nutrient agar following the confirmation of responding to both of casein hydrolysate and methionine. On plating of the original cultures, only one strain gave rise to variants that responded to methionine and not to PABA. The progenies of the other two cultures were of two kinds : one of which responded to both of PABA and methionine and the other responded to neither methionine nor PABA. Of these progenies, one (223-A) was studied further and found to be subject to several types of variation. The variants which were permitted to grow maximally by casein hydrolysate were found to be more stable than the variants having other characteristics. Certain variants which responded to casein hydrolysate responded also to methionine, homocysteine and cystine in this order, some responded only to methionine and others were found to avail none of amino acids tested.
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Juichi OGATA
Article type: Article
1959Volume 18 Pages
591-598
Published: 1959
Released on J-STAGE: December 22, 2017
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2-Methylthiothiamine was an inhibitory antagonist against a thiamine-requiring mutant of Escherichia coli strain 70-23 and the molar ratio of inhibition (inhibitor/thiamine) was of the order of 100-1000. The corresponding 2-ethylthiothiamine showed much less inhibitory potency than the methyl derivative on the same molar ratio. On the other hand, a thiamine-pyrimidine-requiring mutant strain 70-17 could use the methyl derivative alone for growth in place of pyrimidine at the concentrations over 10^<-6> M/l. The mutant was, however, inhibited by the compound in the presence of less than 10^<-9> M/l of thiamine. Pyrimidine moiety of this compound had not the inhibitory activity for both strains and was utilized for synthesis of the compound by their parent strain ATCC 9637. It was, therefore, probable that the compound was competitive for thiamine at the stage of phosphorylation of thiamine or more posterior stage.
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[in Japanese]
Article type: Article
1959Volume 18 Pages
598-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959Volume 18 Pages
598-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 18 Pages
598-
Published: 1959
Released on J-STAGE: December 22, 2017
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Minoru YOSHIDA, Hiroshi HOSHII, Hiroshi MORIMOTO
Article type: Article
1959Volume 18 Pages
599-603
Published: 1959
Released on J-STAGE: December 22, 2017
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The purified synthetic diet shown in Table 1 was given to the day-old White Leghorn male chicks for 3 weeks in the electrically heated battery. The growth of chicks was very poor and various symptoms such as excitability, ataxia and prostration were observed. The growth was however, normal when the amount of vitamins was more than 1.5 times of the original dosage. Comparing the amounts of vitamins given in this experiment with those used by various investigators in the United States and taking the vitamin requirement recommended by National Research Council, U.S.A. into consideration, it seems true that vitamin requirement of Japanese chicks is higher than that of American chicks. Pyridoxine may be one of such vitamins required more. Supplementation of arginine and glycine was effective in increasing the growth rate, but the effect of increase in amount of vitamins was much more remarkable. It may be reasonable to determine the vitamin requirement first and then to study further the amino acids and others.
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[in Japanese]
Article type: Article
1959Volume 18 Pages
603-
Published: 1959
Released on J-STAGE: December 22, 2017
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Osamu TERADA, Kazuo OHISHI, Shukuo KINOSHITA
Article type: Article
1959Volume 18 Pages
604-613
Published: 1959
Released on J-STAGE: December 22, 2017
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The purification of vitamin B_<12> was undertaken in the culture broth of Streptomyces olivaceus and the vitamin was obtained in crystalline forms. Activated charcoal was found to be the most potent adsorbing agent, and conditions of adsorption and elution were investigated. Aqueous solutions of propanol, butanol, benzyl alcohol, acetone and pyridine were found to be exceeding eluting agents. As the intermediary purification process, solvent extraction method with phenol-butanol gave satisfactory results. Resin chromatography with Amberlite IRC-50 was applied as the final purification process wherein a washing with neutral aqueous acetone was found to improve the recovery of pure chromatographic fractions. Purity improvements in purification processes were traced by the changes of absorption curves.
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Osamu TERADA, Kazuo OHISHI, Shukuo KINOSHITA
Article type: Article
1959Volume 18 Pages
614-621
Published: 1959
Released on J-STAGE: December 22, 2017
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Some investigations on the microbiological assay of vitamin B_<12> with L.leichmannii were conducted. On the treatment of samples, the heat extraction under slightly acidic conditions gave better results than the treatment with proteolytic enzymes. Best result was obtained by heating at 100℃ for 15 min. at pH 5.0 in the presence of potassium cyanide and sodium thioglycolate. Improvements of the assay media were undertaken in which the increased addition of organic bases resulted in a remarkable improvement of bacterial responses without the addition of nucleotides as proposed by some workers.
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Tsunemichi MATSUBARA
Article type: Article
1959Volume 18 Pages
622-636
Published: 1959
Released on J-STAGE: December 22, 2017
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Histological and histochemical investigations were undertaken on gonads, chiefly on testicles, of pyridoxine deficient rats. The effects of pituitary gonadotrophine of pyridoxine deficient male rats were also studied. The tisticles or ovaries and accessory sex organs of pyridoxine deficient rats were remarkably atrophic. The atrophy of the testicles of pyridoxine deficient rats could not be completely prevented but slightly restored by gonadotrophine administrations. The atrophy of testicles in the pair weighted control was slight. It was concluded that the atrophy of gonads in pyridoxine deficient rats was caused chiefly by metabolic disturbances in the gonads due to pyridoxine deficiency and partly by dysfunctions of the pituitary and slightly by chronic inanition.
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