VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 10

Displaying 201-250 of 567 articles from this issue

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 232-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_232_2

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STUDIES ON THIAMINASE OF BACILLUS ANEURINOLYTICUS : (V) ISOLATION OF THE DESTRUCTION PRODUCTS OF THIAMINE BY INTRACELLULAR THIAMINASE

Hideo IKEHATA

Article type: Article
1956 Volume 10 Pages 233-234
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_233

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The destruction products of thiamine by Bacillus aneurinolyticus cell suspension were isolated by paper column chromatography : the one was 4-methyl-5-β-hydroxyethylthiazole [yield : 63% as the picrate (mp. 160-161℃)], and the other was 2-methyl-4-amino-5-hydroxymethylpyrimidine [yield : 41% as the free base (mp. 190-194℃), 30% as the HCl-salt (mp. 219℃ (decomp.)].

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STUDIES ON THIAMINASE : (XVII) THE REACTION OF THIAMINASE SOLUTION ON DISULFIDES OF THIAMINE DERIVATIVES AND O, S-ACYLTHIAMINE

Kiku MURATA, Reiko TAKEUCHI

Article type: Article
1956 Volume 10 Pages 235-240
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_235

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According to the results of the experiment on the factors of reduction or hydrolysis of thiamine disulfides or O, S-acylthiamine and thiaminase activity in the thiaminase solution of fern, shell-fish, Bacillus thiaminolyticus Matsukawa et Misawa and Bacillus aneurinolyticus Kimura et Aoyama, it was recognized that heat stable and enzymatic reductive factors and a hydrolytic factor exist in the thiaminase solutions, and that thiamine disulfides and O, S-acylthiamine are at first step reduced or hydrolysed, then decomposed by thiaminase. In comparison between the decomposition grade of thiamine and that of thiamine disulfieds and O, S-acylthiamine in the thiamine solutions, thiamine disulfides are found to be destroyed with more difficultly. Diacetylthiamine is considerably decomposed by the solution of fern and shell-fish which have thiaminase I, but not by bacterial thiaminase solution containing either thiaminase I or II. However, dibenzoylthiamine is generally hard to be destroyed by these thiaminase solutions.

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THE VITAMIN C CONTENTS OF JAPANSES FOODSTUFFS

Kyoko YAMAZAKI

Article type: Article
1956 Volume 10 Pages 240-242
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_240

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For the revision of "Standard Tables of Food Composition in Japan" by Resources Conncil, Prime Minister's Office, 136 kinds of animal and vegetable foodstuffs were analyzed for vitamin C. The true values of vitamin C were determined using ascorbic acid oxidase as well as paper chromatography. Most processed foodstuffs were found to be almost free from vitamin C.

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ISOLATION OF ALKALINE HYDROLYSATE OF RIBOFLAVIN AND CURATIVE EFFECT ON RIBOFLAVIN DEFICIENCY

Chiharu FUKAMACHI, Seiichi HAYAKAWA, Yosito SAKURAI

Article type: Article
1956 Volume 10 Pages 243-246
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_243

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In order to ascertain the growth promoting effect of the alkaline hydrolysate of riboflavin, authors prepared 1,2-dihydro-6,7-dimethyl-2-keto-D-ribityl-3-quinoxaline carboxylic acid and its derivatives. They showed no growth promating effect for albino rat.

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 246-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_246_1

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 246-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_246_2

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 246-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_246_3

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MICROBIOLOGICAL DETERMINATION OF FOLIC ACID AND CITROVORUM FACTOR IN HUMAN BLOOD

Sadamu HIRAIDE

Article type: Article
1956 Volume 10 Pages 247-253
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_247

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Various methods for the release of folic acid and citrovorum factor from their bound forms in human blood were reexamined, and the folic acid and citrovorum factor levels in blood were estimated by the differential microbiological analysis with Streptococcus faecalis and Leuconostoc citrovorum as test organisms. From the data, it was concluded that the enzymatic treatment of blood with hog kidney and chicken pancreas preparations activated by cysteine was the most effective method for the release of folic acid and citrovorum factor from their conjugated forms in blood. With the aid of this treatment, the blood levels of folic acid and citrovorum factor were determined on normal adults. The mean leveles were 8.6±1.6 mγ/ml for folic acid, 2.5±0.9 mγ/ml for citrovorum factor.

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STUDIES ON THE SAWADA'S URINARY TEST

Toichiro SAWADA, Kazuma MIYAJI

Article type: Article
1956 Volume 10 Pages 253-261
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_253

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Our urinary test was found to serve as an index indicating appropriate dosages in the thiamine therapy. In the procedures of the test an intravenous administration of 5ml of 20% fructose solution is most recommendable. A post-administration increase over the pre-administration levels in the values of pyruvic acid and α-ketoglutaric acid in the urine was found to be an indispensable index by which the test should be judged to be positive. In order to avoid possible errors it was advised that, in case the post-administration urine was too much positive for the test, it should be diluted with the pre-administration urine before the result of the test is finally determined.

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 261-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_261_1

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 261-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_261_2

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ANTAGONISM BETWEEN THIAMINE AND SULFA DRUGS IN MICROBES : (I) ANTAGONISM BETWEEN THIAMINE AND ITS STRUCTURAL ANALOG SULFA DRUGS, SULFADIAZINE OR SULFATHIAZOLE IN VITRO

Shuzo KUBO

Article type: Article
1956 Volume 10 Pages 262-267
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_262

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Sulfadiazine and sulfathiazole are most antagonized by thiamine except PABA in Tetrahymena geleii W, Bacillus anthracis, Candida albicans, Bacillus brevis and Bacillus aneurinolyticus. In many microbes, low concentration of sulfa drugs promotes their multiplication in vitro.

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 267-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_267

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THE MICROBIOLOGICAL ASSAY OF VITAMIN B_<12> UTILIZING AUXOTROPHIC MUTANTS OF ESCHERICHIA COLI (I) : A BASIC STUDY

Hiroto IKEDA

Article type: Article
1956 Volume 10 Pages 268-279
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_268

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A basic study to develop a turbimetric assay method for vitamin B_<12> utilizing two mutant strains of Escherichia coli is described. The mineral salts-citrate-gluocse medium of Davis and Mingioli was adapted as the basal medium. The strains employed as test organisms were E. coli #215 and #1544. The former requiring either vitamin B_<12> or methionine was derived from E. coli O-20,and the latter responding to either methionine or homocystine from E. coli ATCC 9537. Both the strains were produced in this laboratory from ultraviolet irradiated cultures of E. coli of wild types by the penicillin technique of Davis and Lederberg et al. The response of #215 to the vitamin was a little better than that of another vitamin B_<12> requiring mutant from ATCC 9637. Methionine interferes with the growth response curve of #215 only when present in amounts greater than 0.7μg per ml. The concentration of methionine, however, can be determined with #1544 as a test organism. The growth of 22 hours incubation was not affected by the presence of amino acids, B-vitamins, and nucleic acids as well as their derivatives, though the organism was stimulated in the presence of vitamin B_<12> during early stage of incubation. The assay of vitamin B_<12> in materials containing methionine and vitamin B_<12> may be possible when diluted to the measureable concentration of the vitamin and less than 0.7μg of methionine per ml of the assay medium.

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 279-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_279_1

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 279-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_279_2

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 279-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_279_3

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THE MICROBIOLOGICAL ASSAY OF VITAMIN B_<12> UTILIZING AUXOTROPHIC MUTANTS OF ESCHERICHIA COLI : (II) VITAMIN B_<12> CONTENT IN HUMAN SERUM

Hiroto IKEDA

Article type: Article
1956 Volume 10 Pages 280-286
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_280

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Vitamin B_<12> in a human serum can be determined by improved technique utilizing two mutant strains of E. coli, one of which, #215,responds to either vitamin B_<12> or methionine and the other, #1544,to methionine or homocystine. (a) Only negligible amounts of methionine are uncovered in a serum after autoclaving it with KCN solution in a acetate buffer of pH 4.6. (b) On the other hand, a large amount of methionine are recovered in a serum treated with papain prior to autoclaving. In this case, vitamin B_<12> and methionine may be assayed after separating them by means of paper chromatography using water saturated n-butanol as a solvent. Results obtained with ten samples were as follows : (a) Serums untreated with papain ; 0.279±0.012mγ/ml. The recovery of vitamin B_<12> added to samples ranged from 87 to 102 per cent. (b) Serums treated with papain ; 0.300±0.025mγ/ml. The recovery of vitamin B_<12> added to samples ranged from 80 to 115 per cent.

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 286-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_286_1

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 286-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_286_2

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 286-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_286_3

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STUDIES ON BACTERIAL SYNTHESIS AND DESTRUCTION OF THIAMINE : (III) SYNTHESIS OF THIAMINE BY WASHED CELLS OF MICROCOCCUS PYOGENES

Fumito SHIMOMURA

Article type: Article
1956 Volume 10 Pages 287-295
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_287

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Washed cells of Micrococcus pyogenes can synthesize thiamine from its pyrimidine and thiazole moieties. The mechanisms of the synthesis are discussed.

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 295-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_295_1

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 295-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_295_2

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THE PROCEDURE OF SAWADA'S URINARY REACTION

Kazuma MIYAJI

Article type: Article
1956 Volume 10 Pages 296-298
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_296

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[title in Japanese]

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Article type: Article
1956 Volume 10 Pages 299-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_299_1

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[title in Japanese]

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Article type: Article
1956 Volume 10 Pages 299-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_299_2

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Article type: Article
1956 Volume 10 Pages 299-300
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_299_3

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[title in Japanese]

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Article type: Article
1956 Volume 10 Pages 300-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_300_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1956 Volume 10 Pages 300-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_300_2

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[title in Japanese]

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Article type: Article
1956 Volume 10 Pages 300-301
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_300_3

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Article type: Article
1956 Volume 10 Pages 301-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_301_1

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Article type: Article
1956 Volume 10 Pages 301-302
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_301_2

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Article type: Article
1956 Volume 10 Pages 302-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_302_1

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Article type: Article
1956 Volume 10 Pages 302-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_302_2

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Article type: Article
1956 Volume 10 Pages 302-303
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_302_3

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Article type: Article
1956 Volume 10 Pages 303-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_303_1

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Article type: Article
1956 Volume 10 Pages 303-304
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_303_2

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 304-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_304_1

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Article type: Article
1956 Volume 10 Pages 304-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_304_2

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Article type: Article
1956 Volume 10 Pages 304-305
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_304_3

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Article type: Article
1956 Volume 10 Pages 305-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_305_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1956 Volume 10 Pages 305-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_305_2

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Article type: Article
1956 Volume 10 Pages 305-306
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_305_3

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[title in Japanese]

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Article type: Article
1956 Volume 10 Pages 306-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_306_1

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[title in Japanese]

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Article type: Article
1956 Volume 10 Pages 306-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_306_2

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[title in Japanese]

[in Japanese]

Article type: Article
1956 Volume 10 Pages 306-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_306_3

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Article type: Article
1956 Volume 10 Pages 306-307
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_306_4

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[title in Japanese]

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Article type: Article
1956 Volume 10 Pages 307-
Published: 1956
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.10.0_307_1

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