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[in Japanese]
Article type: Article
1956 Volume 11 Pages
427-428
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
428-429
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
429-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
429-
Published: 1956
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1956 Volume 11 Pages
430-
Published: 1956
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1956 Volume 11 Pages
430-
Published: 1956
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1956 Volume 11 Pages
430-
Published: 1956
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1956 Volume 11 Pages
430-
Published: 1956
Released on J-STAGE: December 22, 2017
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Article type: Appendix
1956 Volume 11 Pages
430-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
430-
Published: 1956
Released on J-STAGE: December 22, 2017
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Kihachiro UEHARA
Article type: Article
1956 Volume 11 Pages
431-438
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
438-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
438-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
438-
Published: 1956
Released on J-STAGE: December 22, 2017
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Katsuhiko NUMATA
Article type: Article
1956 Volume 11 Pages
439-443
Published: 1956
Released on J-STAGE: December 22, 2017
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For detemining vitamin D in liver oil, the majority of steroids is removed by precipitating in methanol cooled with dry ice, and the vitamin is determined 15 seconds after adding antimony reagent containing acetyl chloride, whereby no effect of a slight amount of steroids remaining is observed. The procedure is thus remarkably simplified. Vitamin D in the eluate from alumina chromatography seemed to have changed into some other substance of a different absorption maximum without losing the recovery of the vitamin activity. Vitamin A is likewise shown to undergo a partial change, appearing partially in the vitamin D fraction in alumina chromatography. Therefore in those cases, where much vitamin A is present together with a small amount of vitamin D, the effect of vitamin A is unavoidable.
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Katsuhiko NUMATA
Article type: Article
1956 Volume 11 Pages
444-449
Published: 1956
Released on J-STAGE: December 22, 2017
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A simple colorimetric method for vitamin D is described, whereby steroids in the unsaponifiable matter are precipitated with cooled methanol and vitamin A is removed by superfiltrol chromatography. Vitamin D is photometerically determined soon after adding antimony reagent. In the presence of more than 500μg of vitamin A, the correction for it is necessary. The limitation of this method for concentration ratio of A/D in I.U. is markedly higher than that of alumina method, the optimum limit of A/D of the former being 20 (but is roughly determinable even at the ratio of 1000), whereas that of the latter about 0.8. In cases, where A/D is below 4,the values of liver oils determined by both methods agree well, but when A/D is above 46,the values with alumina method are remarkably higher than those with superfiltrol method, the former method being distinctly influenced by impurities.
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Motoyoshi MIYAZAKI, Yosito SAKURAI
Article type: Article
1956 Volume 11 Pages
450-457
Published: 1956
Released on J-STAGE: December 22, 2017
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The present report deals with observations on the relationship between vitamin D and rats growth as well as rickets with semi-synthetic diets having various Ca/P ratios, mainly with low-phosphorus diet. The dose of vitamin D promoted the growth of rats fed a diet having a normal Ca/P ratio (1 : 1), and rickets was not observed in the rats on the same diet even without administration of the vitamin. When fed on high Ca and low-P diet (Ca/P 5 : 1 or P≒0), rats showed better growth without administration of vitamin D, while a marked symtom of rickets was observed. The depression of rat growth was caused by the addition of vitamin D on the low-phosphorus diet.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
457-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
457-
Published: 1956
Released on J-STAGE: December 22, 2017
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Isao NAKAMURA
Article type: Article
1956 Volume 11 Pages
458-464
Published: 1956
Released on J-STAGE: December 22, 2017
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The alkaline phosphatase activity of serum, which is one of the valuable indicators for estimating the severity of rickets, was determined following the progression of rickets of albino rats. From the X-ray examination, there has been seen the three stages in rickets, that is, the progressing, stational and worsening. At the first and the third stages, the alkaline phosphatase activity seemed likely to be elevated, but decreased to the normal level at the second or the stational stage. It is considered that the alkaline phosphatase activity of serum is not parallel to the severity of rickets, but rather indicates whether the progress in rickets is active or not.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
464-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
464-
Published: 1956
Released on J-STAGE: December 22, 2017
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Goichiro KATSUI, Takuro MIZUNO, Hiroshi KUYAMA
Article type: Article
1956 Volume 11 Pages
465-467
Published: 1956
Released on J-STAGE: December 22, 2017
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In order to know the relation between antioxidant activity and sulphur in sulphurcontaining organic compounds, investigations were made on the antioxidant activity of a variety of compounds synthesized by the authors such as mercaptan. The results indicated that the compounds having R-SH bond as mercaptan or R-S-R bond had, in general, no antioxidant activity, but the comparison between the compounds having two types of bonds showed a little stronger activity in the former. It was also recognized that the compounds having two sulphur atoms in molecule got a slight antioxidant activity and cyclic compounds having three sulpher atoms had the stronger activity.
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Toshiro SHINAGAWA
Article type: Article
1956 Volume 11 Pages
467-469
Published: 1956
Released on J-STAGE: December 22, 2017
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When ten mg of thiamine per kg of body weight was injected subcutaneously to a rabbit for five days the urinary excretion of both pyridoxine and riboflavin was gradually decreased. When 50 mg/kg of thiamine was injected for three days to the same rabbit five days after the above experiment, the urinary excretion of those two vitamins was remarkably increased at the first day of injection and then dropped sharply below normal.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
469-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
469-
Published: 1956
Released on J-STAGE: December 22, 2017
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Toshiro SHINAGAWA
Article type: Article
1956 Volume 11 Pages
470-473
Published: 1956
Released on J-STAGE: December 22, 2017
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When 10.5mg/kg of thiamineallyldisulfide was injected subcutaneously to a rabbit for four days, the urinary excretion of pyridoxine and riboflavin was gradually decreased and the rabbit died at the last day of injection. When 52.5 mg/kg of thiamineallyldisulfide was injected to another rabbit for two days, the urinary excretion of riboflavin on the first day and that of pyridoxine on the second day were increased sharply and then dropped below normal.
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Yosito SAKURAI, Masakichi KUROGI
Article type: Article
1956 Volume 11 Pages
473-479
Published: 1956
Released on J-STAGE: December 22, 2017
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The flurorescence of riboflavin in weak acidic solution is weakened by the addition of some amino acids, such as tyrosine or tryptophan. When this mixture is irradiated with light, the speed of decomposition of riboflavin is retarded with the decrease of fluorescence, and simultaneously the amino acids above described are decomposed. The same phenomenon was also observed in the addition of phenol, thiourea, ascorbic acid and p-aminobenzoic acid. The fluorescence of riboflavin completely disappeared when the quantity of these substances increased, and no decomposition of riboflavin took place with irradiation. The fluorescent substances other than riboflavin, such as lumichrome, lumiflavin, eosine, fluoresceine or quinine show a similar behavior to riboflavin in that the intensity of fluorescence is affected by the addition of the substances described above with the exception of thiochrome and anthranilic acid.
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Nobuchika ONISHI
Article type: Article
1956 Volume 11 Pages
479-481
Published: 1956
Released on J-STAGE: December 22, 2017
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A perfusion technique of excised dog intestine was employed for the absorption test of riboflavin. The total and the esterified riboflavin absorbed was increased when ursodesoxycholic acid was incubated with riboflavin in the intestinal canal. The remarkably increased absorption of the esterified riboflavin, but not of total, was observed when adenosine and ursodesoxycholate were incubated with riboflavin.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
481-
Published: 1956
Released on J-STAGE: December 22, 2017
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Hiroto IKEDA
Article type: Article
1956 Volume 11 Pages
482-487
Published: 1956
Released on J-STAGE: December 22, 2017
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A suitable method for the maintenance of the stock culture of a mutant strain of Escherichia coli was discussed. When a seed culture is required it is advisable to prepare and store a large number of simultaneous subcultures from one culture of #215,rather than to prepare by repeated serial transfers. Frequent transfers should be avoided. The culture is desirable to be stored in a dark room at a low temperature. When prototrophs are found in such a stock-culture, they can be eliminated as rapidly as possible by penicillin technique. The rescreened auxotrophs should be used as a test organism after being transfered one to two times in a medium containing methionine.
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Hiroto IKEDA
Article type: Article
1956 Volume 11 Pages
487-489
Published: 1956
Released on J-STAGE: December 22, 2017
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The turbidimetric method of the author for the microbiological determination of vitamin B_<12> utilizing the E.coli mutant 215 gave results with 8 samples of human whole blood which were comparable to those obtained by K. Murata and T. Miyamoto of Osaka City University utilizing L. leichmannii method. Mean value of vitamin B_<12> activities of KCN treated blood was 0.297 ± 0.039 mμg/ml for E.coli mutant and 0.251±0.054mμg/ml for L.leichmannii. The value of papain treated blood was 0.323±0.035 mμg/ml for E.coli and 0.260±0.069 mμg/ml for L. leichmannii.
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
489-
Published: 1956
Released on J-STAGE: December 22, 2017
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Goichiro KATSUI
Article type: Article
1956 Volume 11 Pages
490-495
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
495-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
495-
Published: 1956
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
496-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
496-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
496-497
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
497-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
497-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
497-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
497-498
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1956 Volume 11 Pages
498-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
498-
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
498-499
Published: 1956
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
499-
Published: 1956
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
499-
Published: 1956
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
499-
Published: 1956
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1956 Volume 11 Pages
499-
Published: 1956
Released on J-STAGE: December 22, 2017
JOURNAL
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