VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 20

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 409-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_409_2

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STUDIES ON BACTERIAL THIAMINASE : (V) STUDIES ON THE SYNTHETIC MEDIUM FOR BACILLUS THIAMINOLYTICUS

Tadayoshi ONO

Article type: Article
1960 Volume 20 Pages 410-420
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_410

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Investigations were made on the nutritional requirements and simple synthetic medium for Bacillus thiaminolyticus. Ammonium ion was found to be utilized as a nitrogen source, producing thiaminase in the medium, but the nitrogen of nitrite or nitrate was not utilized. Glycerine has a favorable effect on the growth, whereas glucose was proved to be inhibitory. The optimum amount of thiamine for the organism was 0.01 to 0.5 μg/ml of the medium, 10 μg/ml of the vitamin inhibited the growth of almost all strains of the Bacillus tested. A new simple synthetic medium suitable for studying the metabolism of the Bacillus was established. By aerating the culture, the growth was promoted, producing a significant amount of thiaminase.

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STUDIES ON THE AMOUNT OF FREE THIAMINE IN ANIMAL TISSUES IN THE LOADING TEST OF THIAMINE

Osamu NOSE, Masaru KAWAKAMI, Yoshiko IWASAKU, Mitsue WATANABE, Syoko H ...

Article type: Article
1960 Volume 20 Pages 421-424
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_421

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When the animal tissue was kept in the cold and homogenized, the action of phosphatase was inhibited, and a small amount of free thiamine was found. When thiamine and cocarboxylase were injected to albino rats, the amount of free thiamine in liver and kidney was lower in the cold extraction that in that at ordinary temperature. In rats given a large amount of thiamine, the free thiamine contents of liver and kidney were lower in cold extraction procedure than in the extraction at ordinary temperature.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 424-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_424

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DISTRIBUTION OF THIAMINE IN THE SUBCELLULAR COMPONENTS OF RAT-LIVER

Osamu NOSE, Masaru KAWAKAMI, Kenji HIRANO, Junnichi OKUNO

Article type: Article
1960 Volume 20 Pages 425-427
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_425

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Liver cells of rat were divided into three subcellular fractions (nuclear, mitochondrial and soluble fractions) by centrifugation and the thiamine content of each fraction was determined. It was observed that in the soluble fraction the thiamine content was 62% of total thiamine of liver cell, while in the nuclear and mitochondrial fraction it was 24% and 13% respectively.

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EFFECT OF AUREOMYCIN ADMINISTRATION ON HEPATIC ENZYME ACTIVITIES : (I) ON CHOLINE OXIDASE, SUCCINIC DEHYDROGENASE AND TRANSAMINASE ACTIVITIES

Tomoichi KUSUNOKI, Reiji HAYASHI, Syozo ISHIKAWA, Sachiko TANAKA, Keiz ...

Article type: Article
1960 Volume 20 Pages 427-432
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_427

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Rats were administered with Aureomycin 200mg per kilogram body weight daily. Choline oxidase activity of liver homogenate decreased with one and four week administration, while with two and three week administration the activity was at normal level. Succinic dehydrogenase activity decreased slightly with one and four week administration and this was also the case with choline oxidase. These activity alterations of both the enzymes, which are classified as flavin enzyme, were not parallel with the change in the FAD content of liver. No remarkable changes were found in the pyridoxal content and transaminase activity of liver.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 432-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_432_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 432-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_432_2

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EFFECT OF AUREOMYCIN ADMINISTRATION ON HEPATIC ENZYME ACTIVITIES : (II) ON COCARBOXYLASE DEPHOSPHORYLATING AND ALKALINE PHOSPHATASE ACTIVITIES

Tomoichi KUSUNOKI, Reiji HAYASHI, Kenji HIRANO, Hiroshi HATTORI, Kyoic ...

Article type: Article
1960 Volume 20 Pages 433-435
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_433

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Cocarboxylase dephosphorylating activity of liver homogenate of rats, daily administered Aureomycin 200 mg per kilogram body weight, increased during the first week and then decreased in three and four weeks. Alkaline phosphatase activity of liver and kidney homogenates of the rat had no changes with Aureomycin administration.

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CARBOHYDRATE METABOLISM IN PATIENTS WITH BERIBERI AND BERI-BERI LIKE STATE

Eiko IMAI

Article type: Article
1960 Volume 20 Pages 436-446
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_436

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Disturbance of carbohydrate metabolism is an indication of thiamine deficiency. It was found that in patients with beri-beri or beri-beri like state the disturbance was most sensibly detected by a carbohydrate metabolic index (CMI), which was calculated from the levels of pyruvate, lactate and sugar in blood under several conditions. To say nothing of the cases of beri-beri, high values of CMI were also observed in beri-beri like cases in which thiamine deficiency was not apparently detected. The result showed that CMI was useful for finding out the latent thiamine deficiency, though the value was not a specific indicator of the deficiency. For the diagnosis of thiamine deficiency, CMI must be used in combination with the Sawada's urinary test, measurement of blood thiamine content and observation of other clinical symptoms.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 446-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_446

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STUDIES OF THE EFFECT OF STEROID AND ANTIBIOTICS UPON THE PNEUMOCOCCAL INFECTED MICE, PARTICULARLY IN THIAMINE DEFICIENT STATE

Kei ARAYAMA

Article type: Article
1960 Volume 20 Pages 447-455
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_447

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The effect of combined usage of cortisone with penicillin treatment on the experimental pneumococcal septicemia was observed in three groups of immatured mice, thiamine deficient immatured mice and matured mice. Each group was further divided into three groups : the first received 0.5 mg of cortisone, the second 0.05 mg, and the third 0.005 mg, daily. Cortisone was administered after 6,24 and 54 hours after the infection, but 1,000 units of penicillin was injected at 6 and 24 hours. The following results were obtained. Comparing with the penicillin treated animals, cortisone administration was not found to be effective for the pneumococcal infection in all groups. There was no difference of the effects of cortisone between immatured and matured mice. Administration of large doses of cortisone increased the duration of life in all groups, except in the end stadium of thiamine deficiency. In the early stage of thiamine deficiency, when the body weight was still increasing, a resistance to pneumococcal infection was stronger than the control animals, fed with a standard diet.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 455-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_455_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 455-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_455_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 455-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_455_3

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STUDIES ON VITAMIN D_2-PHOSPHATE : (I) SINTHESIS OF VITAMIN D_2-PHOSPHATE AND ITS PHYSICO-CHEMICAL PROPERTIES

Otoharu ISHIZAKA, Hideko ENDO, Takeshi SEKI, Saburo HIRAYAMA

Article type: Article
1960 Volume 20 Pages 456-458
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_456

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The authors have studied the most effective conditions for the phosphorylation of vitamin D_2 and revealed its physico-chemical properties.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 458-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_458_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 458-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_458_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 458-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_458_3

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STUDIES ON VITAMIN D_2-PHOSPHATE : (II) ANTIRACHITIC POTENCY AND TOXICITY OF VITAMIN D_2-PHOSPHATE

Shiro HISADA, Otoharu ISHIZAKA, Toshio NAKASHIMA, Mitsuo TANAKA, Michi ...

Article type: Article
1960 Volume 20 Pages 459-462
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_459

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The authors have studied the antirachitic potency and toxicity of vitamin D_2-phosphate in comparison with vitamin D_2 and revealed that in the former, the antirachitic potency was almost equivalent in molar basis and the toxicity was less than those of free vitamin D_2.

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STUDIES ON THE EFFECT OF A SOUND STIMULATION UPON THE THIAMINE METABOLISM : (I) EFFECT OF A SOUND STIMULATION ON BODY WEIGHT AND AMOUNT OF THIAMINE IN URINE AND THE INTERNAL ORGANS OF RATS

Michio KATO

Article type: Article
1960 Volume 20 Pages 463-479
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_463

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Change of thiamine contents of urine and various organs was examined, when a strong sound stimulation was given consectively for a month to albino rats. The body weight decreased in most cases. The urinary thiamine decreased after a certain time after the sound stimulation. Decreases of the total thiamine content were observed in liver, brain, kidney and intestinal contents, while no appreciable changes were recognized in lung, spinal cord, stomach, intestine, heart, muscle and skin.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 479-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_479_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 479-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_479_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 479-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_479_3

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 479-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_479_4

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STUDIES ON THE EFFECT OF THE SOUND STIMULATION UPON THE THIAMINE METABOLISM : (II) THIAMINE CONTENT OF INTERNAL ORGANS IN RATS, LOADEDWITH THIAMINE AFTER THE SOUND STIMULATION

Michio KATO

Article type: Article
1960 Volume 20 Pages 480-486
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_480

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Sound of 3,000 cps, 115 phone was given on rats consecutively for 8 hours per day for five days and then 0.4 μg of thiamine per g of body weight was subcutaneously injected. The change of thiamine contents of various organs was examined, 30 minutes, 1 and 3 hours after the injection in comparison with the animals without sound stimulation. The difference was relatively remarkable at 3 hours after the injection, total thiamine content decreased in liver, brain, kidney, stomach and blood, and the total thiamine was remarkably low in liver and brain.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 486-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_486

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STUDIES ON THE EFFECT OF THE SOUND STIMULATION UPON THE THIAMINE METABOLISM : (III) EFFECT OF EXCITEMENT BY SOUND ON THIAMINE METABOLISM, IN ANIMALS LACKING AUDITORY AREA IN CEREBRAL CORTEX

Michio KATO

Article type: Article
1960 Volume 20 Pages 487-496
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_487

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With the removal of the auditory area in the cerebral cortex, the thiamine content in the organs of rats showed a slight decrease. Even if excited by sound, however, it did not show further decrease. Noticeable difference of thiamine content was not seen, between the normal and auditory area lacking animals, three hours after the loading of thiamine with sound stimulation.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 496-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_496_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 496-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_496_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 496-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_496_3

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ENRICHMENT OF SOFT DRINKS WITH VITAMIN C : (II) ENRICHMENT OF VITAMIN C TO "RAMUNE", A KIND OF CARBONATED BEVERAGE

Soichi NIWA, Nobu KATAYAMA, Shizuya MATSUBARA, Haruo TANAKA

Article type: Article
1960 Volume 20 Pages 497-500
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_497

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When vitamin C was added in the carbonated drink, it is decomposed by the coexistence of chlorine. It was found, however, that such a decomposition of vitamin C was inhibited by the addition of a small amount of polyphosphoric acid, for instance, in the case of "Ramune" containing 0.3 ppm of Cl_2,the addition of 0.16% of polyphosphoric acid was enough to prevent the decomposition of vitamin C in the drink at 20℃.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 500-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_500_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 500-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_500_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1960 Volume 20 Pages 501-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_501_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 501-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_501_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 501-502
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_501_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 502-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_502_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 502-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_502_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 502-503
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_502_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 503-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_503_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1960 Volume 20 Pages 503-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_503_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 503-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_503_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 503-504
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_503_4

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1960 Volume 20 Pages 504-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_504_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 504-505
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_504_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 505-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_505_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 505-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_505_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 20 Pages 505-506
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_505_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1960 Volume 20 Pages 506-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.20.0_506_1

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