VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 21

Displaying 151-200 of 341 articles from this issue

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1960 Volume 21 Pages 254-255
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_254_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1960 Volume 21 Pages 255-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_255_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 255-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_255_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 255-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_255_3

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 255-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_255_4

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 256-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_256_1

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 256-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_256_2

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 256-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_256_3

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 257-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_257_1

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 257-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_257_2

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 257-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_257_3

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 257-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_257_4

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 257-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_257_5

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 257-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_257_6

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[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 257-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_257_7

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 258-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_258_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 258-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_258_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 258-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_258_3

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 258-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_258_4

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VITAMIN B_2 COMPLEX DEFICIENCY OF CHILDREN

Tsuneo ARAKAWA

Article type: Article
1960 Volume 21 Pages 259-272
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_259

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Ariboflavinosis has been found endemically among the villager at Tsugaru District, Aomori Prefecture. Besides insufficient intake of riboflavin as well as low protein, and high carbohydrate dietary habit, which have been known as causative factors of ariboflavinosis, the well-water, passing through peat-bog widely distributed in this village, was found to exert a profound influence upon riboflavin metabolism. An acceleration of the reaction of FAD→FMN by an addition of the well-water was demonstrated in vitro as well as in vivo. It was probable that the effect of the well-water was brought about mainly by a high concentration of manganese in it. Furthermore, the well-water showed some inhibitory effect upon the intestinal synthesis of riboflavin. Macrocytic anemia, which has been found frequently among children in this village, was considered to be closely related to folic acid deficiency, from the results of folic acid load test, urinary output of formiminoglutamic acid before and after an administration of folic acid and hematologic response to the treatment with folic acid. Nuclear diameters of oral squamous epithelial cells and those of lymphocytes in peripheral blood showed a certain correlation to vitamin B_<12> level in whole blood.

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QUANTITATIVE DETERMINATION OF HYDROXYMETHYLPYRIMIDINE DIPHOSPHATE USING THE SYNTHESIS OF THIAMINE BY CONDENSING ENZYME

Yoshi OKADA

Article type: Article
1960 Volume 21 Pages 273-276
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_273

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 276-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_276

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QUANTITATIVE DETERMINATION OF HYDROXYMETHYLPYRIMIDINE DIPHOSPHATE USING THE SYNTHESIS OF HETEROPYRITHIAMINE BY CONDENSATION WITH PYRIDINE

Yoshi OKADA

Article type: Article
1960 Volume 21 Pages 277-280
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_277

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Synthesis of heteropyrithiamine by condensation of hydroxymethylpyrimidine diphosphate (OMP-PP) and pyridine was applied to the chemical determination of OMP-PP. The heteropyrithiamine formed after heating at 90℃ for 30 minutes with OMP-PP and 1 M pyridine in a final concentration was estimated by alkaline ferricyanide oxidation according to Nose. The reaction product showed a maximal fluorescence at pH 7.0-8.0 with tris buffer. Employing this method, the quantities of OMP-PP ranging from 0.01 to 0.1 μmole can be determined. OMP-monophosphate reacted also with pyridine with a sensitivity about 1/20 fo OMP-PP. The enzymatic OMP-PP formation by thiamine synthesizing enzyme was found to be activated by 64 % after adding NaF to the reaction mixture.

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BIOAUTOGRAPHIC INVESTIGATION ON THE INTERMEDIARY PRODUCTS IN THIAMINE BIOSYNTHESIS, USING ESCHERICHIA COLI MUTANTS

Yoshi OKADA, Katsumi MORI

Article type: Article
1960 Volume 21 Pages 281-286
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_281

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The plausible intermediary products derived from the pyrimidine and thiazole moieties of thiamine in thiamine synthesis by the enzymes extracted from bakers' yeast were investigated bioautographically using Escherichia coli mutants, i.e., pyrimidineless(70-17), thiazoleless (26-43) and thiamineless (70-23) strains. The minimal amount of 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP), OMP-monophosphate (OMP-P) and OMP-diphosphate (OMP-PP) for the growth response of 70-17 strain was 1×10^<-3>, 1 and 1×10^<-1> mμmole respectively, whereas that of 4-methyl-5-β-hydroxyethylthiazole (Th), Th-monophosphate (Th-P) and Th-diphosphate (Th-PP) for the growth response of 26-43 strain was 1×10^<-4>, 1×10^<-3> and 1×10^<-1> mμmole respectively. The detection of OMP-P and OMP-PP in the reaction mixture which had been incubated with the enzyme, OMP (0.1 μmole) and ATP was difficult owing to the contamination of thiamine derivatives contained in the enzyme. However, OMP-PP synthesis was demonstrated bioautographically from the concentrated fraction eluted from a Dowex 1 column. On the contrary, Th-P was detected in the reaction mixture which had been incubated with a partially purified Th-kinase, Th (0.1 μmole) and ATP, whereby the formation of Th-P was roughly estimated to reach 55-60 % of the added Th. Th-PP, however, failed to be recognized.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 286-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_286_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 286-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_286_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 286-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_286_3

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THE VARIATION OF RIBOFLAVIN CONTENTS IN THE ENTRAILS OF ANIMALS AFTER THE ADMINISTERATION OF MEDICINAL SUBSTANCES

Reizaburo SAKUMA

Article type: Article
1960 Volume 21 Pages 287-294
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_287

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The author investigated the influences on the metabolism of riboflavin in albino rats and mice after administeration of antifebriles, sulfamine drugs, antimalarians, soporifics, antibiotics and many other medicinal substances which are used extensively in clinical treatment. It was found that the contents of riboflavin in the entrails of animals had remarkably fallen off after administeration of sulfamine drugs, antimalarians and antibiotics.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 294-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_294

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RIBOFLAVIN CONTENT OF MUSCLES

Reizaburo SAKUMA

Article type: Article
1960 Volume 21 Pages 295-297
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_295

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The author investigated riboflavin in the muscles of higher animals, and ascertained that riboflavin content is larger in antigravity muscles than in contraction muscles and also in red muscles than in white muscles. Riboflavin in limb muscles of rana mugiens increased toward summer and showed the maximum in the middle of August.

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RIBOFLAVIN AND FLUORESCENT SUBSTANCES OF BLATTA ORIENTALIS

Reizaburo SAKUMA

Article type: Article
1960 Volume 21 Pages 297-299
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_297

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Investigation was made on the riboflavin and the fluorescent substances in Blatta orientalis. The riboflavin contents were 11.5 μg/g in male and 6.43μg/g in female and FMN was predominant in both sexes. Fluorescent substances was found at Rf 4.2 and 0.47.

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INFLUENCES OF CARBON AND NITROGEN SOURCES ON THE RIBOFLAVIN OF VEGETABLE TISSUES

Reizaburo SAKUMA

Article type: Article
1960 Volume 21 Pages 299-300
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_299

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The author investigated the influences of carbon and nitrogen sources in hydroponics on the riboflavin contents of vegetable tissues in the growing process of spinach. The ratio C : N=1 : 5 was found to be most effective.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 300-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_300_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 300-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_300_2

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INFLUENCES OF INORGANIC SALTS ON THE FORMATION OF RIBOFLAVIN IN SEEDLINGS OF WHEAT

Reizaburo SAKUMA

Article type: Article
1960 Volume 21 Pages 301-303
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_301

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The author investigated the influences of inorganic salts on the riboflavin content in seedlings of wheat and found that inorganic salts have an important influence on the riboflavin formation, Mg^<++> have accerating and Ca^<++> inhibiting effects.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 303-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_303_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 303-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_303_2

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STUDIES ON THE ALKALI COLOR REACTION OF ASCORBIC ACID : (I) COLOR REACTION OF ASCORBIC ACID IN STRONG ALKALI SOLUTION

Teruko YAMAOKA

Article type: Article
1960 Volume 21 Pages 304-306
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_304

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The present author found that ascorbic acid produces the reddish orange color by BrCN and NaOH. The maximum absorbancy of the color was 520 mμ which changed to 505mμin the course of time. Similar reaction was recognized when iodine, bromine, N-bromosuccinic acid, potassium ferricyanide or ascorbic acid oxidase was used as oxidant in place of cyanogen bromide, simultaneous addition of antioxidant such as KCN or SnCl_2.

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STUDIES ON THE ALKALI COLOR REACTION OF ASCORBIC ACID : (II) APPLICATION OF ALKALI COLOR REACTION FOR THE DETERMINATION OF ASCORBIC ACID

Teruko YAMAOKA

Article type: Article
1960 Volume 21 Pages 307-312
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_307

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The orange-red color, formed by the reaction of ascorbic acid and alkali, was attempted to use as a spectrophotometric determination of ascorbic acid. 0.5% N-bromosuccinimide, 1.5% SnCl_2 and 10 N NaOH are added to the sample solution with 2% metaphosphoric acid containing about 0.5 mg/ml of ascorbic acid, and the color formed is mesured by Beckman spectrophotometer at 520mμ.

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STUDIES ON THE ALKALI COLOR REACTION OF ASCORBIC ACID : (III) DETERMINATION OF ASCORBIC ACID IN PLANT TISSUES BY ALKALI COLOR REACTION

Teruko YAMAOKA

Article type: Article
1960 Volume 21 Pages 312-316
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_312

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The color reaction of ascorbic acid by alkali was applied to the determination of ascorbic acid in plant tissues. Mixed solution of 0.01% thiourea, 1% metaphosphoric acid and 50% ethyl alcohol was found to be more effective for removing the protein and stabilizing the ascorbic acid, increasing the sensibility of the reaction than metaphosphoric acid alone. Ascorbic acid in vegetable tissues was able to determine in an extract containing 0.01 to 0.4 mg/ml of ascorbic acid. The result obtained by the method was almost corresponding to those by indophenol or dinitrophenylhydrazine methods.

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STUDIES ON THE ALKALI COLOR REACTION OF ASCORBIC ACID : (IV) DETERMINATION OF ASCORBIC ACID IN URINE OR IN VARIOUS ORGANS

Teruko YAMAOKA

Article type: Article
1960 Volume 21 Pages 317-322
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_317

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Procedure for the determination of ascorbic acid in urine or various organs was investigated. The inhibitor for the color reaction was found to be removed by passing the extract through the column of Amberlite IR-120. The values obtained by this method were higher than those by the dinitrophenylhydrazine method.

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STUDIES ON THE ALKALI COLOR REACTION OF ASCORBIC ACID : (V) ON THE INHIBITOR OF THE COLOR REACTION

Teruko YAMAOKA

Article type: Article
1960 Volume 21 Pages 322-323
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_322

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Tryptophan, pyrogallol and tannic acid were found to inhibit the color reaction. The formers two are able to be removed by passing the sample solution through the column of Amberlite IR-120,but the latter was not adsorbed by the resin.

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STUDIES ON VITAMIN REQUIREMENTS FOR POULTRY : (I) PYRIDOXINE REQUIREMENT OF WHITE LEGHORN CHICKS

Minoru YOSHIDA, Hiroshi MORIMOTO

Article type: Article
1960 Volume 21 Pages 324-328
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_324

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Pyridoxine requirement of White Leghorn chicks was studied, feeding two strains of day-old male chicks with vitamin B_6-free purified ration containing graded levels of pyridoxine hydrochloride. Maximum growth of a strain of chicks was produced with the ration containing 6.2 mg/kg of pyridoxine hydrochloride and that of the other strain 8.2 mg/kg. Thus it was concluded that vitamin B_6 requirement of our chicks was approximately twice as high as that recommended in U.S.A. Although the data in U.S.A. with White Leghorn chicks and broiler-type chicks indicate the similer requirement of about 3.5 mg/kg, differnce in requirement of the two strains in this experiment was highly significant. Against the old description that vitamin B_6 deficiency lowers the efficiency of feed utilization, feed efficiency was maintained fairly constant until vitamin B_6 content reached as low as 2.2 mg/kg. Vitamin B_6 contents of various feedstuffs common in this country and of cow's and sow's milk were determined microbiologically using Saccharomyces carls-bergensis.

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ON THE TOXIC ACTION OF TOXOPYRIMIDYL ACETYLPYRIDINE

Masanari NAKAGAWA

Article type: Article
1960 Volume 21 Pages 328-334
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_328

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Though toxopyrimidyl acetylpyridine (TXP-AcP) is the condensation product of 3-acetylpyridine (3-AcP) with toxopyrimidine (TXP) its injection causes mice to show symptoms quite different from those aroused by the injection of its components. It has no inhibitory action upon vitamin B_6-dependent enzymes contrary to TXP. The toxic action of TXP-AcP upon yeast alcohol dehydrogenase (ADH) is a little stronger than that of 3-AcP. But the inhibitory action on pyridine enzyme would not explain the mechanism of the toxic action of TXP-AcP on mice because the inhibition on ADH is not prevented by phenobarbital while the death by the injectin of TXP-AcP is fully prevented by phenobarbital. As to cytochrome c oxidase it is inhibited by TXP-AcP but to much weaker extent than by KCN, the ratio of inhibition being below 1 : 100 in molar concentration. As the ratio in lethal dose in mice, however, is 1 : 18 (KCN : TXP-AcP), the mechanism of the lethal intoxication seemed to be other than the inhibition of the cytochrome c oxidase system.

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VITAMIN B_6 METABOLISM IN HIGH TEMPERATURE ENVIRONMENT : (I) CHANGES OF THE CONTENTS OF VITAMIN B_6 AND PYRIDOXIC ACID IN BLOOD AND URINE OF RABBITS AT HIGH TEMPERATURE

Keishi FUJIOKA

Article type: Article
1960 Volume 21 Pages 334-340
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_334

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Determinations of pyridoxine+pyridoxamine, pyridoxal and pyridoxic acid were made in blood and urine of rabbits exposed to high temperature. No significant changes was found in blood after the exposure to 3 hours at 30,35 and 37℃, 12 hours at 30℃ or 24 hours at 35℃. But the blood levels fell after the exposure to 48 hours at 35℃. The urinary excretion of vitamin B_6 and pyridoxic acid decreased by the exposure to high temperature.

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 340-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_340

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VITAMIN B_6 METABOLISM IN HIGH TEMPERATURE EMVIRONMENT : (II) CHANGES OF THE CONTENTS OF VITAMIN B_6 AND PYRIDOXIC ACID IN THE INTERNAL ORGANS OF RABBITS AT HIGH TEMPERATURE

Keishi FUJIOKA

Article type: Article
1960 Volume 21 Pages 341-346
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_341

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Pyridoxine+pyridoxamine, pyridoxal and pyridoxic acid were determined in liver, kidney and brain of rabbits exposed to high temperature. Noticeable changes were not recognized by the exposure to 3 hours at 30 and 35℃, but at 37℃ vitamin B_6 content of the each organ and pyridoxic acid content of liver decreased. Decreases of vitamin B_6 and pyridoxic acid were recognized after the exposure to 12 hours at 30℃ or 48 hours at 35℃ in each organ, by the ordor of brain, kidney, liver, and pyridoxic acid, pyridoxal, pyridoxine+pyridoxamine

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 346-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_346_1

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 346-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_346_2

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[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 346-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_346_3

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