VITAMINS

STUDIES ON THE ENRICHMENT OF SOME FOODSTUFFS WITH VITAMIN B_<12> : (III) ON THE TRANSFER OF VITAMIN B_<12> IN VEGETABLES PICKLED IN FERMENTED RICE BRAN PRODUCTS

Jusaku TAKAHASHI, Yoshikazu SAHASHI

Article type: Article
1960 Volume 21 Pages 466-470
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_466

JOURNAL FREE ACCESS

Show abstractHide abstract

It was studied to elucidate whether vitamin B_<12> diffuses in the pickled radish or cucumber in "Nukamiso", a fermented rice bran products. It was found that radishes were cured slowly in fermented rice bran, and vitamin B_<12> content increased to the maximum after 7 days, while after 4-5 days in cucumbers. Moreover, it was confirmed that vitamin B_<12> added to "Nukamiso" decreased for 1-2 weeks at 30℃ by 46-58% (pH 4.0-3.8) and 44-57% (pH 4.4-4.2). Tests of various stabilizers to "Nukamiso" were also performed, and it was realized that potassium ferricyanide and potassium ferrocyanide were effective for the preservation of enriched vitamin B_<12>.

View full abstract

Download PDF (557K)
EFFECTS OF VITAMIN B_6,γ-AMINOBUTYRIC ACID AND γ-AMINO-β-HYDROXYBUTYRIC ACID ON CANINE CONVULSIONS CAUSED BY THE ADMINISTRATION OF TOXOPYRIMIDINE OR ISONICOTINIC ACID HYDRAZIDE

Hiroaki KOGUCHI

Article type: Article
1960 Volume 21 Pages 470-476
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_470

JOURNAL FREE ACCESS

Show abstractHide abstract

It has been said that toxopyrimidine (TXP) or isonicotinic acid hydrazide (INAH) causes a decreas of the glutamic decarboxylase activity and consequently the reaction product, γ-aminobutyric acid (ABA) or γ-amino-β-hydroxybutyric acid (OABA) is formed. If OABA may be the central nervous depressing substance, the decrease of OABA formation may be the direct cause of the occurrence of convulsions produced by the administration of TXP or INAH. Studies were made on convulsion-depressing and death-preventing actions of ABA, OABA or vitamin B_6 injected into the cisterna magna of a dog in which convulsions had been produced by the administration of TXP or INAH. Although vitamin B_6 exerted a remarkable depressing action on the convulsions, effects of ABA and OABA were not significant. It was, therefore, concluded that OABA might be not a depressor for the central nervous system.

View full abstract

Download PDF (885K)
HISTOCHEMICAL STUDIES ON THIAMINE METABOLISM IN ANTIBIOTIC ADMINISTRATION : (I) DISTRIBUTION OF THIAMINE IN RAT ORGANS IN THE MASS ADMINISTRATION OF AUREOMYCIN

Yuzuru WATANABE

Article type: Article
1960 Volume 21 Pages 476-485
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_476

JOURNAL FREE ACCESS

Show abstractHide abstract

Changes of distribution of thiamine in various organs of rat were investigated histochemically, when 200 mg per kg per day of Aureomycin was orally administered for 3 days to 4 weeks. A marked change was recognized in the liver, for example, in the case of 3 days administration, thiamine decreased remarkably followed by a pathological transformation. When 500 μg of thiamine was given after the Aureomycin administration, the thiamine contents increased in all organs, but the increase in the liver was small. These results indicate that the decrease of thiamine in the organs was not due to the inhibition of bacterial thiamine synthesis in the intestine, bu to the disturbance of thiamine phosphorylation.

View full abstract

Download PDF (2962K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 485-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_485

JOURNAL FREE ACCESS

Download PDF (140K)
HISTOCHEMICAL STUDIES OF THIAMINE METABOLISM IN ANTIBIOTIC ADMINISTRATION : (II) DISTRIBUTION OF THIAMINE IN RAT ORGANS IN THE SMALL AMOUNT OF AUREOMYCIN ADMINISTRATION

Yuzuru WATANABE

Article type: Article
1960 Volume 21 Pages 486-491
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_486

JOURNAL FREE ACCESS

Show abstractHide abstract

Similar studies as previous report were conducted when 20 mg per kg per day of Aureomycin was administered. A remarkable increase of thiamine in the organs, especially in the liver was observed. The growth of the rat administered with Aureomycin was promoted than that of the control. In the thiamine loading, the increases of thiamine were more evident in liver, kidney and small intestine. These results indicate that the increase of thiamine in the organs was due not only to the stimulation of thiamine synthesis by intestinal bacteria but also to the promotion of phosphorylation of thiamine in the liver by the administration of Aureomycin in small quantity.

View full abstract

Download PDF (1015K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 491-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_491

JOURNAL FREE ACCESS

Download PDF (164K)
HISTOCHEMICAL STUDIES OF THIAMINE METABOLISM IN ANTIBIOTIC ADMINISTRATION : (III) DISTRIBUTION OF THIAMINE IN THE ORGANS OF PNEUMOCOCCUS INFECTED RAT IN AUREOMYCIN ADMINISTRATION

Yuzuru WATANABE

Article type: Article
1960 Volume 21 Pages 492-498
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_492

JOURNAL FREE ACCESS

Show abstractHide abstract

The thiamine contents of various organs of rats which died 2 to 4 days or were killed one week after the Pneumoccocus infection, were compared to those of 3 days or one week after the Aureomycin administration. A marked changes were found in the heart, followed by liver, kidney and spleen. The pathological transformations were found in these organs. In the case of 3 days administration of 50 mg per kg per day of Aureomycin, the thiamine contents of the organs were almost unchanged, even increased in the one week administtration. The reduction of thiamine content by Pneumococcus infection is provably due to the pathological changes. It is postulated that the change of thiamine content by Aureomycin may be due to the prevention of pathological changes and to the metabolic hyperfunction by restoration mechanism.

View full abstract

Download PDF (2016K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 498-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_498

JOURNAL FREE ACCESS

Download PDF (157K)
ON THE REVELATION OF RIBOFLAVIN DEGRADATION PRODUCT IN PLANT TISSUE

Kazuo HOTTA, Osamu ANDO

Article type: Article
1960 Volume 21 Pages 499-502
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_499

JOURNAL FREE ACCESS

Show abstractHide abstract

When the above ground part of farm grown pea plant was water cultured in riboflavin solution several days, a violet blue fluorescent substance, having the Rf value of 0.45 (butanol・acetic acid・water=4 : 1 : 5) was found in its leaves. This substance is identified as flavoviolet (1-ribityl-2-oxo-3-hydroxy-6,7-dimethylquinoxaline) which was previously isolated by Hotta et al. from the products in a alkaline pyrolysis of riboflavin.

View full abstract

Download PDF (488K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 502-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_502

JOURNAL FREE ACCESS

Download PDF (157K)
STUDIES ON THE RIBOFLAVIN CONTENT OF POLLENS

Kiyoko TAKIGUCHI, Kazuo HOTTA

Article type: Article
1960 Volume 21 Pages 503-505
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_503

JOURNAL FREE ACCESS

Show abstractHide abstract

Living mature pollen contains two or more times riboflavin than in other parts of plant. In germinative pollen the riboflavin contained is always of ester form, being mostly FMN, in less degree FAD, but no free form. When pollen has lost germinating power, the decomposition of FAD is seen to take place with considerable rapidity, and diminishing of FMN is observed, both presumably undergoing a change from ester form to free one.

View full abstract

Download PDF (378K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 505-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_505

JOURNAL FREE ACCESS

Download PDF (165K)
INFLUENCE OF VITAMIN B_6 ON THE BIOSYNTHESIS OF THE THYROID HORMONE OF THE RAT

Kozo YAMADA, Shuhei OGAWA, Tomohiro SHIN

Article type: Article
1960 Volume 21 Pages 506-508
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_506

JOURNAL FREE ACCESS

Show abstractHide abstract

It has been already demonstrated that the synthesis of organic iodine compounds by surviving slice of thyroid tissue of the rat was inhibited by pyridoxal phosphats, pyridoxal, pyridoxine phosphate and pyridoxamine. In this paper the effect of pyridoxal phosphate, pyridoxal and pyridoxamine phosphate for the synthesis of thyroid hormones in the rat in vivo, respectively was examined. It resulted that pyridoxal phoshate, pyridoxal and pyridoxamine phosphate did not influnce on the synthesis of monoiodotyrosine and diiodotyrosine, but that pyridoxal phosphate inhibited the biosynthesis of thyroxine. Pyridoxal and pyridoxamine phosphate did not inhibit it.

View full abstract

Download PDF (346K)
STUDIES ON THE MECHANISM OF RIBOFLAVIN BIOSYNTHESTS BY ASHBYA GOSSYPII : (II) EFFECTS OF TEMPERATURE ON RIBOFIAVIN-BIOSYNTHESIS AND GROWTH OF THE CELL

Hiromichi ONOZAKI

Article type: Article
1960 Volume 21 Pages 508-515
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_508

JOURNAL FREE ACCESS

Show abstractHide abstract

Experiment are performed on whether any correlation exists between the incubation temperature and biosynthesis of riboflavin or carbohydrate metabolism in the cells of Ashbya gossypii. There is a remarkable difference in the riboflavin biosynthesis at different temperatures of submerged culture. When the temperature is raised from 27 to 37℃, the riboflavin formation decreases greatly. But the oxidation velocity of sugary substances, except pentoses, and the organic acids contained in TCA-cycle members by the resting cells are apparently greater at 37 than at 27℃. The inhibitory effects of NaF, CH_2ICOOH and streptomycin on the glucose and organic acids metabolism are also generally greater at higher temperatures. The RQ in the glucose oxidation at 37 and 27℃ is 1.31 and 1.0 respectively. From the results obtained, it may be concluded that A. gossypii only produces riboflavin at a temperature which is lower than the optimum for the carbohydrate metabolism.

View full abstract

Download PDF (621K)
FORMATION OF THIOCHROME : (I) OXIDATION OF THIAMINE BY FERRICYANIDE OR CYANOGEN BROMIDE AND DETECTION OF ITS REACTION PRODUCTS BY PAPER PARTIRION CHROMATOGRAPHY

Takatomo HORIO

Article type: Article
1960 Volume 21 Pages 515-519
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_515

JOURNAL FREE ACCESS

Show abstractHide abstract

Thiamine solutions (about 5%) of different pH values between 5 and 12 were oxidized by ferricyanide or cyanogen bromide and their reaction products were separated by paper partition chromatography (butanol・H_2O) and detected by means of Dragendorff reagent or fluorecence. Ferricyanide-oxidation in the alkaline range showed always presence of thiamine-disulfide beside thiochrome which was detected much more distinctly after the solution had been made strongly alkaline. Cyanogen bromide to thiamine solution of pH values below 11.4 brought thiochrome as the major product and at pH between 10.7 and 11.6 cyanothiamine as the most distinct spot on paper partition chromatography, whereas at pH over 12 thiamine-anhydride and thiamine-disulfide were detected. Thiamine was first transformed into cyanothiamine in the alkaline medium by cyanogen bromide, then in the more alkaline solution cyanothiamine was decomposed to thiamine-anhydride or combined with an another molecule of thiamine forming thiamine-disulfide. In the quantitative estimation of thiamine by ferricyanide yield of thiochrome was 72-74% of the theoretical value and in nitrogen-saturated water, the yield was increased to 80-84% : Thus dissolved oxygen would have favored to form thiamine-disulfide instead of thiochrome.

View full abstract

Download PDF (555K)
FORMATION OF THIOCHROME : (II) RELATION BETWEEN pH AND FORMATION OF THIOCHROME FROM THIAMINE BY CYANOGEN BROMIDE

Takatomo HORIO

Article type: Article
1960 Volume 21 Pages 520-524
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_520

JOURNAL FREE ACCESS

Show abstractHide abstract

Thiochrome was formed by adding cyanogen bromide into buffered thiamine solutions of different pH values between 6 and 12. Thiochrome was estimated by measuring its absorption maximum at 365mμ or the fluorescence of its butanolicextract. The formation of thiochrome was highest at pH 9〜11,and quicker at pH 10〜12 than at pH 7〜9. If the buffered thiamine solution was kept standing for one hour before adding cyanogen bromide to it, thiochrome was only obtained at pH less than 9.5,while cyanothiamine and thiamine disulde were formed at pH more than 10. Thiochrome was still attainable at neutral or slightly acidic medium even though the reaction was slow and imperfect. The solution of thiamine monohalide such as thiocyanate could be transformed into thiochrome by cyanogen bromide without NaOH to the extent of 30-40% yield on warming. This suggested that cyanogen bromide enabled to form thiochrome from pseudobase of thiamine and cyanothiamine from thiol form of thiamine.

View full abstract

Download PDF (540K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 524-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_524_1

JOURNAL FREE ACCESS

Download PDF (165K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 524-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_524_2

JOURNAL FREE ACCESS

Download PDF (165K)
STUDIES ON FATTY ACID ESTERS OF FLAVINS : (II) RIBOFLAVIN TETRACAPRATE AND RIBOFLAVIN TETRABUTYRATE

Kunio YAGI, Jun OKUDA, Toshiro MATSUBARA

Article type: Article
1960 Volume 21 Pages 525-527
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_525

JOURNAL FREE ACCESS

Show abstractHide abstract

Following to the synthesis of riboflavin tetrapalmitate, a fat-soluble riboflavin derivative, we intended to synthesize other fatty acid esters of riboflavin to apply them to nutritional field. Riboflavin caprate and butyrate were prepared in the similar way as riboflavin tetrapalmitate. The absorption spectra and fluorescence emission spectra of their alcoholic solution were estimated, and their Rf values for various solvents were also obtained. Then, chemical structure of the above two derivatives were studied by the estimation of riboflavin and fatty acid in their molecules, and by the test of periodate oxidation. The experimental results showed that these two derivatives are riboflavin-2', 3' 4', 5'-tetracaprate and riboflavin-2', 3', 4', 5,' tetrabutyrate.

View full abstract

Download PDF (361K)
STUDIES ON THE INJURY OF REPRODUCTIVE AND ENDOCRINE ORGANS IN VITAMIN E DEFICIENCY

Shih Chih LEE

Article type: Article
1960 Volume 21 Pages 528-537
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_528

JOURNAL FREE ACCESS

Show abstractHide abstract

Histopathological studies were made on the changes in the reproductive and endocrine organs of rats, fed on a vitamin E deficient diet. Marked degeneration and atrophy were observed in the testis. These changes were considered to be produced by the inhibition of spermatogenesis following the necrosis of spermatogonia. Electronmicroscopic studies revealed that the degeneration and necrosis of spermatogonia were caused by the degeneration and destruction of the mitochondria and endoplasmic reticulum of the testis. A remarkable proliferation of interstitial cells occurred with the degeneration and atrophy of the seinal tubes. From the electronmicroscopic pattern, it was concluded the endoplasmic reticulum concerns the granule formation in the interstitial cells. Changes of the hypophysis and adrenal systems were also investigated and the significance of the systems in vitamin E deficiency was discussed.

View full abstract

Download PDF (3982K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 537-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_537

JOURNAL FREE ACCESS

Download PDF (166K)
A STUDY OF PATHOLOGICAL EFFECTS OF OROTIC ACID ON RATS

Shin Chih LEE

Article type: Article
1960 Volume 21 Pages 538-541
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_538

JOURNAL FREE ACCESS

Show abstractHide abstract

Studies were made on the effect of a heavy administration of orotic acid to rats (0.5,1 or 5g/kg) for 2 months. Neither of macro- and microscopic changes were observed in the organs, and the growth of the animals was not affected also by the administration of the substance. Then, it was concluded that the toxity of orotic acid is not so significant.

View full abstract

Download PDF (411K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 541-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_541_1

JOURNAL FREE ACCESS

Download PDF (170K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 541-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_541_2

JOURNAL FREE ACCESS

Download PDF (170K)
[title in Japanese]

[in Japanese]

Article type: Article
1960 Volume 21 Pages 541-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_541_3

JOURNAL FREE ACCESS

Download PDF (170K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 542-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_542_1

JOURNAL FREE ACCESS

Download PDF (69K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 542-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_542_2

JOURNAL FREE ACCESS

Download PDF (69K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 542-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_542_3

JOURNAL FREE ACCESS

Download PDF (69K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 542-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_542_4

JOURNAL FREE ACCESS

Download PDF (69K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 543-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_543_1

JOURNAL FREE ACCESS

Download PDF (89K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 543-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_543_2

JOURNAL FREE ACCESS

Download PDF (89K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 543-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_543_3

JOURNAL FREE ACCESS

Download PDF (89K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 543-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_543_4

JOURNAL FREE ACCESS

Download PDF (89K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 543-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_543_5

JOURNAL FREE ACCESS

Download PDF (89K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 543-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_543_6

JOURNAL FREE ACCESS

Download PDF (89K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 544-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_544_1

JOURNAL FREE ACCESS

Download PDF (99K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 544-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_544_2

JOURNAL FREE ACCESS

Download PDF (99K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 544-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_544_3

JOURNAL FREE ACCESS

Download PDF (99K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 544-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_544_4

JOURNAL FREE ACCESS

Download PDF (99K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages 544-
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_544_5

JOURNAL FREE ACCESS

Download PDF (99K)
[title in Japanese]

Article type: Appendix
1960 Volume 21 Pages App2-_z_
Published: 1960
Released on J-STAGE: December 22, 2017

DOIhttps://doi.org/10.20632/vso.21.0_App2

JOURNAL FREE ACCESS

Download PDF (2805K)

Register with J-STAGE for free!