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Jiro NOZAKI
Article type: Article
1959Volume 16 Pages
516-518
Published: 1959
Released on J-STAGE: December 22, 2017
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Investigations were made on the preventive effect of glucuronate, glycine and B_6-group vitamins parenterally administered for the convulsion caused by an injection of INAH. It was found that 4-5mg/g of glucuronate was effective against the convulsion caused with the injection of 0.15mg/g of INAH, that every compound tested was ineffective against the convulsions caused with more than 0.2mg/g of INAH, and that B_6-group vitamins were ineffective against all such convulsions. Of the B_6-group vitamins, pyridoxal was found to shorten the time from the injection of INAH to the appearance of the convulsion.
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Masakazu IZUMIYA
Article type: Article
1959Volume 16 Pages
518-522
Published: 1959
Released on J-STAGE: December 22, 2017
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Riboflavin was adsorbed on a free form of strongly acidic cation exchange resin and all anion exchange resins. FMN and FAD were adsorbed slightly on a free form of strongly acidic cation exchange resin and perfectly on a free-and salt-form of anion exchange resins. FAD was easily adsorbed on any form of anion exchange resins and desorbed with sodium salycilate, sodium trichloroacetate and sodium chloride solution from them. From 3ml, each of Amberlite IRA-401-Cl saturated with FAD, the space velocity of flow rate being adjusted to 4,FAD was eluted 51% and 75% with 5 and 10% sodium chloride solution, respectively.
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Masakazu IZUMIYA
Article type: Article
1959Volume 16 Pages
523-526
Published: 1959
Released on J-STAGE: December 22, 2017
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Some purifying effects were found on a process of adsorption and desorption of crude FAD preparations on a column of Amberlite IRA-401-Cl. Ion exchange resin required in this process was below 100 ml per g of FAD, and we consider it to be effective as a unit process of FAD production.
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[in Japanese]
Article type: Article
1959Volume 16 Pages
526-
Published: 1959
Released on J-STAGE: December 22, 2017
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Masakazu IZUMIYA
Article type: Article
1959Volume 16 Pages
527-531
Published: 1959
Released on J-STAGE: December 22, 2017
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FAD was extracted with pyridine-methanol-water mixture from wet mycellia of Eremothecium ashbyii and uptaken on a column of Amberlite IRA-401-Cl. After washing the column with a dilute aqueous solution of sodium chloride, FAD was desorbed with a 10% sodium chloride solution, vacuum concentrated, extracted with phenol, adding ether, reextracted with water and precipitated with ethanol. In this method, FAD preparations of 86.5% purity were obtained in a yield of 49.1% from mycellia cultured in a fermentor of 2 tons in capacity.
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Masakazu IZUMIYA
Article type: Article
1959Volume 16 Pages
531-535
Published: 1959
Released on J-STAGE: December 22, 2017
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FAD was adsorbed on several commercial active carbon powder, with the capacity of from 10 to 15% of their weight. It was eluted with basic solvent more easily than with acidic solvents. Among them, the optimum ratio of pyridine-water was 1/1 and that of lutidine-water was 4/6. Addition of a small amount of amines, quinolines or nitrobenzene to pyridine-water (1/1) facilitated elution, but it was more excellent with lutidine-water (4/6). FAD was adsorbed on active carbon and the recovery of 80% or more was obtained with 5 times elutions using lutidine-water (4/6) of 10 fold volume of carbon weight. No purifying effect was observed in the above process.
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Masakazu IZUMIYA
Article type: Article
1959Volume 16 Pages
535-538
Published: 1959
Released on J-STAGE: December 22, 2017
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Depositing conditions were observed with Ag, Hg, Cd, Ba, Ca, Zn and Pb-salts of FAD. FAD with nearly 90% purity was obtained from crude FAD through a process forming Ba- and Ag-salt and liberating the metal ions. It was concluded that purifying effect was principally due to the Ag-salt process, the yield of which was about 70% including the succeedng process.
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[in Japanese]
Article type: Article
1959Volume 16 Pages
538-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959Volume 16 Pages
538-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
538-
Published: 1959
Released on J-STAGE: December 22, 2017
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Hideo IKEHATA
Article type: Article
1959Volume 16 Pages
539-541
Published: 1959
Released on J-STAGE: December 22, 2017
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It was observed that partly purified thiaminase II of Bacillus aneurinolyticus catalysed the formation of thiothiamine from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 2-mercapto-4-methyl-5-β-hydroxyethylthiazole, and that thiothiamine could be used for the growth of Bacillus aneurinolyticus, instead of thiamine.
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[in Japanese]
Article type: Article
1959Volume 16 Pages
541-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959Volume 16 Pages
541-
Published: 1959
Released on J-STAGE: December 22, 2017
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Tadayoshi ONO
Article type: Article
1959Volume 16 Pages
542-545
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959Volume 16 Pages
545-
Published: 1959
Released on J-STAGE: December 22, 2017
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Chikataro KAWASAKI, Kozo OKADA
Article type: Article
1959Volume 16 Pages
546-552
Published: 1959
Released on J-STAGE: December 22, 2017
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Thiamine contents and concentrations of livers, hearts, kidneys and intertines of rats on normal, thiamine-rich or thiamine-deficient diet were determined by thiochrome method after the animals had been perfused in situ with 0.85% saline solution through jugular veins. It was shown that thiamine concentrations of livers and intestines of both normally-fed and thiamine-rich rats had significant differences between nonperfused and perfused animals, whereas no significant differences between them were observed on thiamine-deficient rats. The percentage of phosphorylated thiamine to total thiamine contents of organs underwent no appreciable change after perfusion. The perfusion of isolated rat livers showed a great outflow of thiamine at the initial 30 minutes, then gradual decrease between 30〜120 minutes and still a small quantity of thiamine-outflow even after 120 minutes. Surface-active agents such as Igepon T, Span 60 or quarternary ammonium chloride, added to the saline solution at 20〜50 mg % concentrations on the last stage of ordinary perfusion had little effects to drain off further amounts of thiamine from livers.
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Noriaki KUWANA, Shoichi SHIMIZU
Article type: Article
1959Volume 16 Pages
552-556
Published: 1959
Released on J-STAGE: December 22, 2017
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The present paper deals with the fundamental investigations on the paper electrophoretic separation of a series of compounds of the vitamin B_<12> groups. In this research were used crystalline cyanocobalamin and almost pure preparations of-Factor A, Factor B, Factor III and pseudovitamin B_<12> that were kindly furnished by Dr. K. Bernhauer. Satisfactory separation of all these factors except Factor III was attained under the following conditions ; electrolyte : 0.5 or 1.0 N acetic acid containing 0.01% KCN, electrode vessel solution : 1% boric acid, electric current density : 0.1〜0.3 mA/cm, potential gradient : 10〜20 V/cm, paper : Toyo filter paper No. 50,time operated : more than 2 hours (but, more than 5 hours' migration is desirable in case of bioautographic detection.
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Noriaki KUWANA, Shoichi SHIMIZU
Article type: Article
1959Volume 16 Pages
556-560
Published: 1959
Released on J-STAGE: December 22, 2017
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Previous reports from our laboratory have shown that the bacterial cell fractions obtained from the supernatant liquor of night soil treatment plant and distillers' wastes digester have high microbiological activities for vitamin B_<12>. We were, however, afraid that these cell fractions might contain some vitamin B_<12> factor other than cyanocobalamin. This paper is devoted, therefore, to the paper electrophoretic examination of vitamin B_<12>-like compounds in the newly found resources. The results demonstrated that pseudovitamin B_<12>. Factor A, Factor B and a compound supposed to be Factor C are contaminated in the extract of cell fraction, but that 70〜80% of the total activity is due to cyanocobalamin and Factor III.
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[in Japanese]
Article type: Article
1959Volume 16 Pages
560-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
560-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
560-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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Kazuo SATO
Article type: Article
1959Volume 16 Pages
561-564
Published: 1959
Released on J-STAGE: December 22, 2017
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Satisfactory result was obtained in the determination of vitamin B_6 in multi-vitamin preparations by improving the method of Hrdy et al., using diethyl-p-phenylenediamine. In this method, the reaction was not inhibited by vitamins of B-group, while the presence of vitamin C showed the interference which was removed by iodine treatment.
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[in Japanese]
Article type: Article
1959Volume 16 Pages
564-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
564-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
564-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
564-
Published: 1959
Released on J-STAGE: December 22, 2017
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Tutomu MOTEGI
Article type: Article
1959Volume 16 Pages
565-575
Published: 1959
Released on J-STAGE: December 22, 2017
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Research was made on serial and individual fluctuations of the nicotinic acid metabolism in healthy body by measuring the nicotinic acid level in blood and the excretion of nicotinic acid, N'-methylnicotinamide and N'-methyl-3-carboxylamide-6-pyridone in urine. (1) The average nicotinic acid level in blood of 12 men and 12 women was 0.46±0.080 mg/dl, and the individual or serial fluctuation was rather small. (2) The average excretion of nicotinic acid in urine per day (12 men and 10 women) was 2.4±0.71mg, which had some relation with the nicotinic acid level in blood, and it showed less individual fluctuation than that of N'-methyl-nicotinamide and N'-methyl-3-carboxylamide-6-pyridone. (3) The average excretion of N'-methylnicotinamide in urine per day (12 men and 10 women) was 4.6±1.47mg and that of N'-methyl-3-carboxylamide-6-pyridone (12 men and 6 women) was 9.1±2.98mg. The individual and dietary fluctuations were remarkable. (4) Proportions of excretion of the above three compounds in urine were nicotinic acid 100,N'-methylnicotinamide 192,and N'-methyl-3-carboxylamide-6-pyridone 379.
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[in Japanese]
Article type: Article
1959Volume 16 Pages
575-
Published: 1959
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1959Volume 16 Pages
575-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
575-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
575-
Published: 1959
Released on J-STAGE: December 22, 2017
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Kazuo HOTTA, Shuichiro INA
Article type: Article
1959Volume 16 Pages
576-579
Published: 1959
Released on J-STAGE: December 22, 2017
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By the photolysis of riboflavin in alkaline medium, lumiflavin was formed which is decomposed further to 3,4-dihydro-3-keto-4,6,7-trimethyl-2-quinoxaline carboxylic acid (lumiflavin-keto acid) by alkali. Lumiflavin-keto acid is further converted methyloxoviolet after 94 hours at room temperature in the presence of hydrogen peroxide in alkaline solution. Methyloxoviolet was proved to be identical with 4,6,7-trimethyl-2,3-dioxotetrahydroquinoxaline synthesized from 1,2-dimethyl-4-amino-5-methylaminobenzene.
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Kazuo HOTTA, Shuichiro INA
Article type: Article
1959Volume 16 Pages
580-584
Published: 1959
Released on J-STAGE: December 22, 2017
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Lumichrome which was formed by the photolysis of riboflavin in the presence of hydrogen peroxide in neutral or weak acidic medium, was decomposed in sealed tube with alkali to 3,4-dihydro-3-keto-6,7-dimethyl-2-quinoxaline carboxylic acid (lumiflavin-keto acid). By standing lumiflavin-keto acid at room temperature in alkaline solution in the presence of hydrogen peroxide, it is formed a bluish violet substance having Rf 0.80,which was proved to be 6,7-dimethyl-2,3-dioxotetrahydroquinoxaline. Lumiflavin-keto acid was transformed by sublimation at 250-270℃ to 3,4-dihydro-3-ketcr6,7-dimethylquinoxaline. The last compound was converted by methylation in alkaline solution by dimethyl sulfate to 3,4-dihydro-3-keto-4,6,7-trimethylquinoxaline.
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[in Japanese]
Article type: Article
1959Volume 16 Pages
584-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
584-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
584-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
584-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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Kazuo HOTTA, Shuichiro INA
Article type: Article
1959Volume 16 Pages
585-589
Published: 1959
Released on J-STAGE: December 22, 2017
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When 6,7-dimethyl-2,3-dioxotetrahydroquinoxaline (oxoviolet) and 4,6,7-trimethyl-2,3-dioxotetrahydroquinoxaline was treated with dimethyl sulfate in alkaline solution, the same methylated compound was obtained which was proved to be 1,4,6,7-tetramethyl-2,3-dioxotetrahydroquinoxaline (methyloxoviolet). When 6,7-dimethyl-4-D-ribityl-2,3-dioxotetrahydroquinoxaline (flavoviolet)was treated with dimethylsulfate in alkaline solution, 1,6,7-trimethyl-4-D-ribityl-2,3-dioxotetrahydroquinoxaline was formed which yielded methyloxoviolet by treating with hydrogen peroxide in a hot alkaline solution.
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[in Japanese]
Article type: Article
1959Volume 16 Pages
589-
Published: 1959
Released on J-STAGE: December 22, 2017
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Kazuo HOTTA, Shuichiro INA
Article type: Article
1959Volume 16 Pages
590-594
Published: 1959
Released on J-STAGE: December 22, 2017
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The authors synthesized 3,4-dihydro-3-keto-6,7-dimethylquinoxaline-2-carbonamide, 3,4-dihydro-3-keto-6,7-dimethylquinoxaline-2-carbohydrazide, 3,4-dihydro-3-keto-6,7-dimethyl-2-quinoxaline-carboxyureide, 4,6,7-trimethyl-3,4-dihydro-3-oxo-2-quinoxaline-carboxyureide, 6,7-dimethyl-3,4-dihydro-3-oxo-4-D-ribityl-2-quinoxaline-carboxyureide as the allied compounds of the decomposition products of lumichrome-keto acid, and studied the chemical and physical properties of these compounds and the derivatives of their carboxyureides.
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 16 Pages
595-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 16 Pages
595-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 16 Pages
595-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1959Volume 16 Pages
595-596
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 16 Pages
596-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1959Volume 16 Pages
596-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
596-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1959Volume 16 Pages
596-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 16 Pages
596-597
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1959Volume 16 Pages
597-
Published: 1959
Released on J-STAGE: December 22, 2017
JOURNAL
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