1) It was discussed about RI compound using in cisternography and usefulness of RI-cisternography for diagnosis. 2) It could be known that Yb-DTPA was favorable pharmaceutical agents for RI cisternography in a physico-chemical property. 3) Abnormal findings were found on cisternogram of subdural hematoma, subarachinoidial bleeding, infratentorial tumor, or normal pressure hydrocephalus In these diseases, it could be considered that abnormal findings depended upon a disturbance of movement or absorption of the spinal fluid. Therefore, scintiphotogram taken in series was important for diagnosis of RI-cisternography.
Various thiamine analogues with or without vitamin activity were tested to see whether vitamin activity is related to anti-inflammatory effect. The anti-inflammatory properties of thiamine, its related substances (biologically active, inactive, or antagonistic) and the modified radicals per se freed from the molecule of the active compounds were examined using (1) rat paw edema, (2) capillary permeability and (3) granuloma assay. These agents were compared with known anti-inflammatory drugs in the effect. Thiamine (300 mg/kg, i.p.) showed no effect in rat paw edema induced with carrageenin and formaldehyde. Oxythiamine—a thiamine antagonist—and disulfide-typed thiamines, e.g. TDS (thiamine disulfide), TTFD (thiamine tetrahydrofurfuryl disulfide) and NTDS (O-nicotinyl thiamine disulfide) were all effective in anti-edema test. Furan-ring freed from TTFD molecule failed to show anti-edema effect, while nicotinic acid showed potent effect. However disulfide-typed thiamines exerted no suppressive effect on increased capillary permeability induced with histamine and bradykinin. TDS, oxythiamine and nicotinic acid inhibited the granuloma induced with filter-paper discs. However, this effect of oxythiamine seemed false, because the animals on it lost body weight. By any methods, no distinct antiinflammatory effects were shown with desthio-thiamine and thiaminic acid, which have no vitamin activity. From these, it was clarified that anti-inflammatory effects of thiamine derivatives are related to either the vitamin activity or the chemical structure. But we found that antiinflammatory potency vary remarkably according to various experimental methods and various radicals bound to the thiamine disulfide structure.
In the evaluation of the functional alterations of the kidneys occurred in the hemorrhagic shock, the renogram is very important. In this study the renogram and hemodynamics in acute hemorrhagic shock were investigated and infusions effect to hemorrhagic shock was observed using renogram. Then it was concluded that renogram is one of the valuable examination's method in the evaluation of renal function in hemorrhagic shock. And the following results were obtained. 1) The angle formed by RTT and SEGIII had markedly related to the amounts of artifical hemorrhage. 3) The time distribution of99mTcO4-within the kidney were varied in the different amount of artifical hemorrhage. 3) The combination's infusion of low molecular weight dextran and blood transfusion was showed an excellent effect to renal blood flow in hemorrhage shock.
In order to study the hypoxic influence of hemolysis, 0.5 ml/kg of phenylhydrazine (P.h) was injected subcutaneously into rabbits every three days at a concentration of 2%, 4% and 8%, respectively. The rabbits were then sacrificed on a specified day, and the spleens were examined histologically. 1. Acute group: The spleen showed primarily edema and hemorrhage due to congestion, with scattered foci of necrosis and mild extramedullary hematopoiesis. The deposition of hemosiderin was comparatively marked, and it was noted that there was a slight tendency towards increased pigment deposition with an increase in dosage of P.h. 2. Chronic group: The splenic parenchyma contained areas of focal necrosis and fibrosis, accompanied by diffuse fibrous proliferation and disruption of the splenic architecture. The hemosiderion deposition was further marked. The arterioles showed sclerotic changes, and foci of extramedullary hematopoiesis were noted. 3. While various hematologic changes result from the red blood cell destruction in the spleen and the hemolytic anemia induced by P.h administration, the morphologic changes of edema, necrosis, and fibrosis are caused by a permeability disturbance of the sinusoidal wall and small blood vessels of the spleen due to the hypoxic influence. It is considered that the degree of morphologic change will vary directly with an increase in P.h dose.