By injecting ricin to fertilized hen's eggs before incubation and 5 days after the incubation, the author investigated the toxicity of ricin and its influences exercised on the development of chick embryo and on its allantoic fluid. The author further investigated the histopathological changes in the liver and kidney of the chick embryo to obtain the following conclusions.
1) Toxicity: In the cases when injected before the incubation, all of the eggs died on the 9 th day of incubation with 100γ and 1, 000γ. However, with the amounts less than 10γ, perfect hatching was proved successful though deaths were recorded in certain number of eggs. In the cases when the injections were given 5 days after incubation, all of the eggs died by the 12 th day of incubation with the amount of 1γ and 10γ. However, with the amounts less than 0.1γ, perfect hatching was proved successful though a few deaths were recorded.
2) Development of chick embryo: In the cases of pre-incubation injections, no remarkable difference was noted in the initial stage of incubation. However, in the terminal stage of incubation, injections of lower concentrations promoted the development of chick embryo, while those of higher concentrations acted rather inhibitorily. When injected 5 days after the incubation, no remarkable difference was generally observed.
3) Allantoic fluid: When injections of various concentrations were given before the incubation, the amount of allantoic fluid was found smaller in comparison with the controls in the initial stage of the incubation. Also in the terminal stage of the incubation, the excretion of urine was found inhibited when lower concentration were given, but was bound promoted when higher concentration were given. When injected 5 days before the incubation, the injection acted inhibitorily in the initial stage of the incubation. However, in the terminal stage, the excretion was larger than in the controls injected with 0.01γ, and smaller than the controls when injected with 0.1γ.
4) Findings in the liver: In the cases of the injections before the incubation, the hyperemia of the central veins and the blood vessels of the Glisson's capsules were remarkable. As the concentration of ricin increased, the regressive degenerations of hepatic cells such as vacuolar degeneration, fat degeneration and necrobiosis or necrosis were recognized. In the cases when the injections were given 5 days after the incubation, hyperemia of the central veins and the blood vessel of the Glisson's capsules were noted, but the fat degeneration of the hepatic cells was recognized only in the terminal stage of the incubation. Furthermore, when the findings in the cases with the injections before the incubation and those in the cases after the incubation (0.1γ-0.01γ) were compared, the regressive degeneration in the hepatic cells was stronger in the former cases.
5) Findings in the kidney: In the case when the injection was given before incubation, regressive degenerations in the urinary tubules and the congestion of the interstitial blood vessels were recognized. Particularly in some of those with a high concentration, hyperemia and round cells infiltration of the glomerulus were noted. In the case when the injection was given 5 days after the incubation, almost nc changes were recorded in the glomeruli, and granular degeneration of the urinary tubules was observed. Further, when the findings in the kidney in the cases of the injections before the incubation, injections 5 days after the incubation, respectively injected with 0.01γ and 0.1γ, were compared, it was learnt that the regressive degenerations in the urinary tubules in the initial stage of the incubation were found stronger when injections were given 5 days after the incubation, and that, in the terminal stage, they were found more remarkable when injections were given before the incubation.
抄録全体を表示