Fertilized hen's eggs which were administered glutamylcholine were incubated. Findings on 9th and 12th day of the incubation are as follows : Slight hyperplasia of Kupffer's cells was observed, and in a few cases which were administered high concentration of glutamylcholine, granular degeneration and necrobiosis were seen. The shape of Mitochondria were various, but generally fine and changed to bacilliform and number of them increased with the lapse of incubation period. Findings on 15th and 18th day of the incubation are as follows : Disarrangement and regressive changes of hepatic cells such as necrosis and necrobiosis were observed. Findings on 18th day were more remarkable than the others. Mitochondria were decreased in number, being the shape varied, and a part of them were disappeared.
By the injection of glutamylcholine into fertilized hen's eggs, the following results were obtained. In the general development of the chick embryo any tendency of promotion was not observed. No influence was observed on the amount of the allantoic fluid in the early stage of the incubation, while in the late stage the excretion of the allantoic fluid was inhibited slightly. A considerable change in nitrogen content cccured in the allantoic fluid.
The excitatory process of single nerve fiber of frog caused by electric stimulation have been studied. Hashida have been already mentioned that there were two stages in the process of nervous reaction following electric stimulation. The first stage was the passive process, namely stimulatory process, and the second was that of the active one, namely excitatory process. The stimulatory process advanced during “Nutz Zeit” and the excitatory process advanced and finished during the latent period of the action potential following “Nutz Zeit”. The latent period shortened increasingly by continuous electric stimulation of the same strength following “Nutz Zeit”. The minimum of the shortened period was 0.05-0.1 cosec. On the contrary after the “Nutz Zeit” when electric current applied on the opposite direction, the prohibiting action was observed and the excitatory process was inhibited or even stopped. But when the stimulation applied after a certain period, no matter how strong the stimulation was, the prohibiting action did not occur. From these observation we found that there were two stages in the excitatory process which made progress from the end of the “Nutz Zeit” to appearance of action potential. We pressume that the electrical mechanism acted in the first stage and the chemical process did in the second stage.
The action potential of the lateral nerve of Carussius aurats was recorded by the cathode ray oscilloscope. The action potential showed two or more peaks, and the first of which was stronger. The conduction velocity of the first spike potential was 28.9 m/sec, and that of the second was 9.5 m/sec. The lateral nerve was consisted from at least two kinds of fiber groups, i. e. A and B. The nerve fibers which conduct the first spike potential belongs to A group, while the second belongs to B. The lateral nerve contains more than 900 fibers of various diameter (25μ-1μ) . According to Erlanger and Gasser's assumption, which insists the proportionality between the conducting velocity and the cross area of fibers, we could assume the appearance of three peaks of action potential theoretically. The expeumeutal results fuvoured this theoreticul postulation.
The influence of nicotine upon the chick embryos, especially the growth of bone (long bone) was investigated histopathologically. Applying 1.0 mg, 5.0 mg and 7.0 mg of nicotine per one egg, the eggs were opened on the 10 th, 15 th and 20 th day of incubation. In measuring thier humerus and femur, inhibition of growth was recognized in comparison with the control, especially remarkable in high concentration of nicotine. The same could be said with the body weight of the chick embryo. A decrease of osteoblasts, undergrowth of cart silage periosteum, degeneration of cartilage bone-cells and nuclei, alternation of slightness of bonebeam and incomplete absorption of cartilage and others were recognized. These findings ware especially remarkable in the egg applied with a high concentration of nicotine. However, the systematic alternation in the eggs opend on the 20 th day was milder than those opened on the 15 th day, and it was more significant in cartilage than in bone. (So that the influence of nicotine was more remarkable on cartilage) In the present experiment, the imperfect development of the bones by nicotine should be classiified as a hyperlastic type of Chondrodystrophia foetalis.
Since the report on bronchial adenoma was presented by Wesler-Rabin in 1932 this problem has become a point of interest to many investigators, and among these the study on its histological features, histogenesis and malignancy by Foster-Carter is outstanding. However, there have been only 8 cases of bronchial adenomas reported in our country. The authors had the opportunity of examining the surgical material of a 28 yr. old male diagnosed as pulmonary tumor. Gross findings : Intrabronchial growth of tumor was found in the following branches ; Truncus bronchalis superior sinister, R. medius lateralis, R. medius sinister, R. apicalis ventralis. On the other hand, there is invasion of the tumor in the lung parenchyma with partial hemorrhage and necrosis. The above lesion is encapsulated by a fibrous tissue and the adjacent parenchyma is atelectatic. Bronchiectasis is found in the peripheral area andd colloid-like substance fills these cavities. The tumor is firm, milky white with hemorrhagic spots. Microscopical findings : The surface of the tumor showing intrabronchial growth is covered by normal bronchial epithelia, and necrosis and hemorrhage are sometimes observed. The tumor cells demonstrate the following arrangments : 1. solid-cord type, 2. Swiss-cheese-like adenomatous type and 3, papillary adenomatous type.
The high plasma-fibrinogen value in cancer patients and its decrease after cancer treatment of any kind were observed. The entity of the high value of the plasma-fibrinogenn is not yet clear, but we assume that some specific relations may exist between high fibrinogenn value and cancer toxin.
Our statistical observations on the acidity of human gastric juice were as follows. 1. Incidence of anacidity gradually increased with age, and a quater of it revealed in so called cancer-age group. 2. Incidence of anacidity existed 12.5% in 287 cases of gastric ulcer, 3.4% in 59 of gastric ulcer associated with duodenall ulcer, and 1.4% in 141 of duodenal ulcer. 3. Incidence of anacidity in 961 cases of gastric cancer was 67.3%. But in 138 cases of tumors whose diameters were below 5 cm it was only 45.7%. 4. There was no connection between an acidity and location of gastric cancer, but, in localized type it showed anacidity more frequently than in infiltrating type. 5. In ulcer-cancer free-HCI secretion was relatively well preserved even in advanced case.
Tetraethylammonium, Guanidine and Veratrine, applied on the skelettal muscle, caused elevatation of irritability of terminal fibers of motoneuron in that muscle. As its result in the terminal fibers, repetitive excitations were evoked by a single stimulus. When a single stimulus was applied to a muscle directly, the muscle showed the repetitive excitation, because the action current of a muscle stimulated the terminal fiber antidromically as a ephapse, and then spontaneous excitation occured in the terminal fiber. propagated to the muscle fibers again, and evoked the repetitive contracticns. Frcm above mentioned facts, it should be concluded that the terminal fiber of motoneuron has a different physiological characteristics from an axon.
EDTA with the diet caused accelerating effects on the formation of tumor or cirrhosis in liver induced by DAB, while the diet containing OX inhibited the tumor induction slightly. The formation of liver cirrhosis in mice was accelerated by administration of EDTA with the diet containing azo compounds. Skin cancer developed early when chelating compounds added to the diet. Administration of chelating compounds With the diet had no effect on the growth of Ehrlich's ascites tumor in mice.
The distribution of human blood groups examinated in our department in the last one year is as follows. Over 3000 peresons, mainly lived in Tokyo, were examined : O type-34.34%, ±0.847 A type-35.58%, ±0.854 B type-22.34%, ±0.734 AB type-7.73%.±0.477 Thiss distribution is not coincide with the average of Japanese, while Tokyo-people have been gathered from all over Japan. In the anthropological studies we pay attention on the distribution of human blood groups.
We have experienced a case of braintumor of Glioblastoma multiforme in a child. Microscopically, the mature glial fibrillar network was not prominent and not be determined as aproliferation of uniform afibrillar medulloblast. In Hortega-staining methode, tumorcells stained with slight bizarr. Polar gliohlasts with some process or differentiation-grade like mature astrocytes were observed. Their structure was not uniform. Atypia, giant cells and nuclear abnormality, especially large nuclei were observed. Mitosis was scare or lacking. We assume that this is an immature neoplasm showing differentiation to astrocytes. This might be a case of a Glioblastoma multiforme.
Present paper deals with the studies on the bile soluble character of Brucella melitensis. Whole cultures of strains 11M and 14M of Br. melitensis were non-sensitive to bile or phenol. Some of colonies of Br. melitensis formed on a plate were sensitive slightly to bile of phenol. When the sensitive colonies were subcultured repeatedly, especially on glycerol agar medium, their sensitivity was intensified. When this strain was subcultured on liver agar, some of colonies loses their sensitivity. Both of the above sensitive and non-sensitive strains were identical completely in agglutination reaction, heavy metal ion reaction with 10% CuSO4 or Millon's reagent, resistance to the dyes of Huddleson, growth characteristics on Petragnani's slants, urease activity, H2S gas production, growth inhibition by DEDTC patch of Renoux, colonial appearance and etc, except their reaction to bile or bile salts. This was suggesting that they were both belong to the genus Brusella and were related as a original and a variant. Namely, the bile soluble character of Br. melitensis discussed in this paper was not necessarily to be one of the essential characters described by Benoki. It was recognized only when bile sensitive variants were formed on glycerol agar medium by repeated subcultures of some bile non-sensitive strains. Further discussions on the above mentioned problems will be made in the following report (Keio Igaku, 35, 668, 1958) which was done by employing Mexican strains of Br. melitensis.