A Colorimetric method, using
p-nitrobenzaldehyde and
o-dinitrobenzene, combined with the diffusion analysis was established for the determination of cyanide in food.
After the addition of 0.4ml of the absorbing reagent (0.15N KOH) into the inner chamber and then 1-2ml of sample solution (distillate containing 0-150ng CN) and 2ml of 10% H
2SO
4 solution into the outer, the standard unit was allowed to stand for 3 hours at 30-35°C. The content of the inner chamber was treated with chromogenic reagent (0.25ml of 0.1M
p-nitrobenzaldehyde methylcellosolve solution and 0.25ml of 0.1M
o-dinitrobenzene methylcellosolve solution) and allowed to stand for 30 minutes at room temperature.
After diluting the colored solution with 5ml of methylcellosolve, the absorbance at 560mμ of the solution was measured.
The content of cyanide (CN):
0-464ppm (42 samples) for Beans.
0.014-129ppm (14 samples) for Apricot kernel.
0.008-0.018ppm (3 samples) for Corn.
0-0.013ppm (5 samples) for Tapioca.
38.2-44.5ppm (4 samples) for Chips of Cassava root.
0-3.4ppm (15 samples) for Fruit wine.
0 (10 samples) for “Sake”, Whisky and Brandy.
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