食品衛生学雑誌
Online ISSN : 1882-1006
Print ISSN : 0015-6426
ISSN-L : 0015-6426
8 巻, 4 号
選択された号の論文の12件中1~12を表示しています
  • -食肉および乳に起因する人畜共通伝染病-
    今泉 清
    1967 年 8 巻 4 号 p. 299-306
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
  • 興津 知明, 河端 俊治, 小嶋 秩夫
    1967 年 8 巻 4 号 p. 307-310
    発行日: 1967/08/05
    公開日: 2010/07/27
    ジャーナル フリー
    フリルフラマイドの殺菌作用について, E. coli 2株, Staph. aureus 2株を用いて検討を行ないつぎの結果を得た.
    1. pH 7.0のnutrient broth中と緩衝液中とを比較した結果, 4株中3株はnutrient broth中の方が殺菌作用が強かった.
    2. 培地のpHは6.0の方が8.0よりやや殺菌作用が強い傾向を示し, また糖の存在は殺菌作用をやや弱くする傾向を示した.
    3. 殺菌作用は0~40°の間では, 温度が高いほど著しかったが, Q10は20~40°の間で1.2~1.8と比較的低い値を示した.
  • 水分ならびに原料油脂のビタミンA, β-カロチンの安定性に及ぼす影響
    今村 正男, 新谷 いさお, 兼松 弘, 飯島 紘
    1967 年 8 巻 4 号 p. 311-317
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    In order to examine the correlation between the stability of both Vitamin A and β-carotene in margarine and moisture together with oils used as raw material, margarine were prepared with various amounts of water, coconut oil and cotton seed oil both of which were raw material oils, and then their preservability was examined at 5°C and 25°C for 12 months.
    As a result, it was found that the more the moisture content, the worse became the preservability of vitamin A and β-carotene, and especially this tendency was remarkable in the case of storage at 25°C and in the samples containing liquid oil. And those containillg coconut oil showed an increased stability, but, on the contrary, the survival rate of vitamin A and β-carotene markedly decreased with the increase in the amount of cotton seed oil.
  • 浜口 彰, 松木 幸夫, 田中 喜作
    1967 年 8 巻 4 号 p. 318-324
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    We studied on the determination of the preservatives in foodstuffs by ultraviolet absorption after dialysis pretreatment.
    At this experiment five kinds of preservatives were taken up, namely benzoic acid (BA), dehydroacetic acid (DHA), p-hydroxy benzoate (POBE), salicylic acid (SA) and sorbic acid (SOA).
    Results obtained were as follows;
    1. A correcting formula was useful to remove interferences resulted from foods.
    2. Quantitative analysis of the mixture of preservatives are conducted, making use of variations of absorption values that were caused by the reduction with Raney-Nickel and Zinc or the oxidation with either hydrogen peroxide or potassium permanganate.
  • 都市川水からの腸炎ビブリオの検出例とその分離操作上の問題
    安永 統男
    1967 年 8 巻 4 号 p. 325-330
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    The main purpose of the present work was to detect Vibrio parahaemolyticus from river water not mixed with sea water in other area than Nagasaki-city, from river water of which a great number of halophilic bacteria including the vibrio have been isolated in the previous work, in the light of a report that a few attempts to detect the vibrio from river water of other areas basing on the author's report were unsuccessful.
    In this work, the samples (river water) were collected from rivers in Sasebo-city (Table 1), and three broth media were employed for enrichment culture of the water samples (Table 2). The isolation of the vibrio were carried out by means of primary or secondary isolation procedures (Fig. 1).
    From these experiments, it was shown that the vibrio was isolated from river water in Sasebo-city as was in Nagasaki-city. This indicated that the isolation of the vibrio from city river water might not be unusual. The comparative results by the three broth media revealed that media containing ethylviolet or super teepol, which have been recognized to be highly effective as selective substances for sea water sample, were either incomplete or inhibitory for the isolation of halophilic bacteria including the vibrio from river water samples (Tables 3 and 4). It was also noted that the bacteria of biotype 2 and bacteria closely related to biotype 1 were isolated by means of secondary isolation procedure from their parent cultures which were obtained by primary isolation procedure and could grow in NaCl-not-containing peptone water (Table 5). The latter bacteria are placed in the V. parahaemolyticus group in the present paper. It is interesting that all of these bacteria indicated hemolytic activity by the method of Kato et al.
  • 近藤 龍雄, 川城 巌, 古橋 詩子
    1967 年 8 巻 4 号 p. 331-334
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    A Colorimetric method, using p-nitrobenzaldehyde and o-dinitrobenzene, combined with the diffusion analysis was established for the determination of cyanide in food.
    After the addition of 0.4ml of the absorbing reagent (0.15N KOH) into the inner chamber and then 1-2ml of sample solution (distillate containing 0-150ng CN) and 2ml of 10% H2SO4 solution into the outer, the standard unit was allowed to stand for 3 hours at 30-35°C. The content of the inner chamber was treated with chromogenic reagent (0.25ml of 0.1M p-nitrobenzaldehyde methylcellosolve solution and 0.25ml of 0.1M o-dinitrobenzene methylcellosolve solution) and allowed to stand for 30 minutes at room temperature.
    After diluting the colored solution with 5ml of methylcellosolve, the absorbance at 560mμ of the solution was measured.
    The content of cyanide (CN):
    0-464ppm (42 samples) for Beans.
    0.014-129ppm (14 samples) for Apricot kernel.
    0.008-0.018ppm (3 samples) for Corn.
    0-0.013ppm (5 samples) for Tapioca.
    38.2-44.5ppm (4 samples) for Chips of Cassava root.
    0-3.4ppm (15 samples) for Fruit wine.
    0 (10 samples) for “Sake”, Whisky and Brandy.
  • 第1報 かん詰腐敗細菌に対するナィシンおよびタイロシンの抗菌作用
    田中 昭二, 小野 輝昭, 松田 典彦, 宮井 久子
    1967 年 8 巻 4 号 p. 335-338
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    Antibiotic effect of Nisin and Tylosin on 78 sporeforming bacteria (53 Bacilli and 25 Clostridia) and 24 non-sporeforming bacteria (5 Lactobacilli, 6 Staphylococci, 6 Escherichia, 4 Aerobactor and 3 Micrococci) were investigated.
    Thermophilic spores bearing rods such as B. stearothermophilus, B. coagulans, Cl. nigrificans and Cl. thermosaccharolyticum were more effectively inhibited with Nisin than such mesophilic species as B. subtilis and B. cereus. Similar results were obtained with Tylosin. However, anaerobic spoilage bacteria (Clostridia) were more sensitive to Tylosin. Most of non-spore formers were little or not inhibited with Nisin and Tylosin.
  • 清酒中の水銀含量について
    近藤 龍雄
    1967 年 8 巻 4 号 p. 339-340
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    The content of mercury was determined in “Sake”, rice for “Sake” production, “Sake” cake etc. by means of dithizone method.
    Results obtained are summarized as follows:
    1. The content of memcury: Below 0.02ppm (11 samples) for “Sake”. Below 0.10ppm (8 samples) for rice for “Sake” production.
    0.05ppm-0.77ppm (6 samples) for “Sake” cake.
    Below 0.57ppm (9 samples) for rice bran (white).
    0.32-1.55ppm (10 samples) for rice bran (brown).
    2. It was found that mercury content in rice for “Sake” production was low and the residual mercury in it was accumulated in the “Sake”, cake.
  • 斎藤 真, 神保 芳郎
    1967 年 8 巻 4 号 p. 341-344
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    The growth rate of psychrophilic and mesophilic bacteria in raw milk stored at low and room temperature was studied.
    The results were as follows:
    1. The maximum number of psychrophilic bacteria in raw milk stored at 5-7°C reached 108-109per ml after 5-7 days and the time required to reach the maximum count of the bacteria was affected by their initial counts. The similarity of the growth rates was observed for psychrophilic and mesophilic bacteria when stored at 20-25°C.
    2. Colony forming percentage of the strains isolated from the culture placed at 5-7°C for 7 days was 54% when maintained at 37°C for 2 days.
    The majority of isolated psychrophilic bacteria were gram-negative, catalase-positive rods.
  • 井上 哲男, 加藤 三郎, 大河原 晃
    1967 年 8 巻 4 号 p. 345-350
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    Gas chromatographic procedure for the determination of sodium cyclamate was studied. According to recent studies, it has been reported that sodium cyclamate reacts with nitrite and produces cyclohexanol, cyclohexanone and cyclohexene. The author's experiment showed that the major product of the reaction was cyclohexyl nitrite, together with some cyclohexanol and slight cyclohexene in the presence of excess nitrite at temp. below 5°C.
    Using the blended liquid phase column consisting of DC-550 and DEGS (10% of each) on Gaschrom P (60/80mesh) at 60°C, carrier gas nitrogen (60ml/min) and a flame ionization detector, cyclohexyl nitrite and cyclohexanol were investigated. The results are given in Fig. 1- (C) and 2.
    Sodium cyclamate was determined on the basis of the cyclohexyl nitrite. The cyclohexyl nitrite was prepared by treating varying amounts of sodium cyclamate with 5ml of 10% sodium nitrite and 4ml of 10% sulfuric acid in ice water and it was extracted with 10ml of n-hexane containing 0.25 v/v% n-decane as internal standard and then injected to the Shimazu gas chromatograph.
    The relation between peak heights of cyclohexyl nitrite and amounts of sodium cyclamate was linear in the range of 20-600mg of the latter, and in this case, cyclohexanol did not appeared. But, when excessive amounts (>800mg) of sodium cyclamate were present, the yield of cyclohexyl nitrite diminished and that of cyclohexanol increased. The minimum limit of detection was approximately 0.1μg as sodium cyclamate.
  • 楢府 直大, 早川 順子, 高橋 弘明, 石田 康雄
    1967 年 8 巻 4 号 p. 351-357
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
  • 天野 立爾
    1967 年 8 巻 4 号 p. 358-366
    発行日: 1967/08/05
    公開日: 2010/03/01
    ジャーナル フリー
    原因の明らかになった食中毒事件数の95%はサルモネラ食中毒であるが, その中毒患者数は前年の1964年よりわずかに減少した. しかしウェルシュ菌, ブドウ球菌食中毒患者は前年より増加している. しかもウェルシュ菌食中毒は規模の点について見るとサルモネラ菌食中毒より大きいものが多い.
    卵製品, 食肉製品, 飼料, 肥料等から種々のサルモネラ菌が分離されており, Taylorらはそれらの菌について血清型による分類を行なっている. 肉類によるサルモネラ食中毒の場合には同じ血清型あるいはファージ型の菌が食肉店あるいはと殺場から見つかるケースがしばしばある. また家畜の研究機関による家畜間のサルモネラ感染症の調査によれば, 人間の食中毒の場合に分離されるものと同じ血清型のサルモネラ菌が見いだされることが多いという. この菌による食中毒は原因食品をつきとめることが困難なことが多いが, 肉類はそのうちで一番多く原因食品となっており, 牛, 豚およびと殺場などはサルモネラ菌の感染源となって広範囲に食中毒を起こさせていると考えられる. 1965年中に農場が直接汚染源となったサルモネラ菌食中毒は未殺菌牛乳によるもの6件, 家畜からの直接接触感染による食中毒26件が報告されている.
    食肉製品はまたウェルシュ菌食中毒のすべての原因食品となっており, その患者数は1964年より約1,000人増加した. この食中毒はほとんど酒保その他の大規模な給食設備を有するところにおいて, あらかじめ調理しておいた肉料理が原因で発生している.
    ブドウ球菌による食中毒患者数は前年より非常に増加したが, その原因はコック等の食品取扱い者からの直接感染が原因になった3例の大きな集団食中毒によるところが大きい.
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