滬紙電気泳動法によつて罹病植物のたんぱく構成またはその変化などを追求する場合, 共存する植物病原菌菌体に含まれるたんぱくを無視できない場合もあると考えられるので, P. oryzae など8種類の植物病原菌の泳動たんぱくについて二, 三の実験を行なつた。 P. oryzae, C. miyabeanus などの菌糸原形質のたんぱくは原点部を含む少なくとも三つの泳動分劃から構成されているが, C. sasakii, S. bambusicola では四つの分劃がみとめられた。 P. oryzae など3種の菌では, それぞれ菌系統の間に泳動図の質的な差はみとめられない。 P. oryzae 菌糸の泳動たんぱくは培養が古くなるにしたがつて減少し, 6週間以後は全く消失する。 10-4モルの重金属溶液 (硫酸銅または昇汞) に20時間接触させた P. oryzae 菌糸の泳動たんぱくは変性を受けて, その泳動図が変化する。
1) Effect of Blasticidin S on glycolysis in Piricularia oryzae was-examined by measuring lactic acid according to the method of Backer Sammerson, using the suspension of washed mycelium prepared by the method described previously. The glycolysis was almost not inhibited by Blasticidin S, but was remarkably inhibited by phenyl mercuric acetate and monoiodoacetate. 2) Eflect of Blasticidin S on succinic dehydrogenase in cell free extract of the mycelium of Piricularia oryzae was examined by the method in which TTC reduction was measured by the Thunberg technique. Blasticidin S had no significant influence on the succinic dehydrogease. 3) Effect of Blasticidin S on the oxidative phosphorylation by mitochondrial fraction from rat liver was examined. Oxygen consumption was measured manometrically and phosphorylation was estimated by the determination of inorganic phosphate. The oxygen uptake and the oxidative phosphorylation by the rat liver mitochondria were not affected by Blasticidin S, but both reactions were inhibited by phenyl mercuric acetate and Blastmycin. 4) Effect of Blasticidin S on the turnover of inorganic phosphate by respiring mycelium of Piricularia oryzae was studied with the aid of exogenous inorganic phosphate labelled with 32P. 32P from labelled phosphate in the medium was taken up more into the nucleic acid fraction than into the phospholipid or the protein fraction of the mycelium. Each fraction was isolated by the method of Schneider. It was found that the incorporation of 32P into each of the fractions was not influenced by Blasticidin S. 5) Addition of 14C-glutamic acid to the suspension of the mycelium above mentioned resulted in the incorporation of 14C into the mycelium. Since 14C-glutamic acid was incorporated more into the protein fraction than into the nucleic acid or the phospholipid fraction, the incorporation was taken as a measure of examinig protein synthesis. The incorporation into the protein fraction of the mycelium was strongly inhibited by Blasticidin S at a concentration of 1μg/ml. Because this inhibitory concentration was almost equal to the minimum growth inhibitory concentration (0.1-1.0μg/ml), it seemed that the inhibition of protein synthesis was a primary effect of Blasticidin S on the metabolism of Piricularia oryzae.