ミツバ株枯病菌(Fusarium oxysporum 860926a)を抗原として,モノクローナル抗体API9-2およびAPI9-3を得た。これらのモノクローナル抗体は,抗原であるF. oxysporum 860926aをはじめとしてF. oxysporumの試験した11のすべての分化型,Gibberella fujikuroi (F. moniliforme), G. zeae (F. roseum), Nectria haematococca (F. solani)およびN. solani (Thielaviopsis basicola)と強い陽性反応を示した。供試したこれ以外の35種の植物病原菌とは,反応がみられなかった。このように,本モノクローナル抗体はほぼFusarium属菌に特異的に反応した。
A new disease of perilla (Perilla frutescens) broke out in Oita Prefecture in 1990. The symptoms of the disease appeared on leaves and stem as brown spot. Corynespora sp. was isolated from infected lesions. Conidia were hyaline to pale brown, obclavate to cylindrical, curved, broad at the base, formed on conidiophores singly or in chain. Conidiophores were pale to mid brown and erect. No stromata were observed. The optimum temperature of mycelial growth lied at 27.5-30°C. The fungus was pathogenic to 17 species including tomato, cowpea, sesame and others among 18 tested plants in inoculation experiments. Based on these morphological and pathological characters, the causal fungus was identified as Corynespora cassiicola (Berk. & Curt.) Wei by the similarity to the description of Ellis (1971).
Leaf spot and leaf decay disease of perilla (Perilla frutescens) has been found on harvested perilla leaves at market of Osaka in summer season of 1990. The spots of perilla leaf were circular and black, sometimes leaves were decayed. Isolated fungi formed singly or in chains of conidia on an apex of conidiophore on perilla leaf. Conidia were straight to curved, cylindrical to obclavate. The isolated fungi infected soybean, lotus lily, eggplant, and several tropical plants which described in Wei's report. According to these characteristics, the fungus was identified as Corynespora cassiicola (Berk. & Curt.) Wei. We propose the name of Corynespora leaf spot disease of perilla.