わが国で初めて,オオムギ黄枯病菌として3種のPythium属菌(P. spinosum,P. ultimum var. ultimum, P. sylvaticum)を同定した。そのうちP. spinosumとP. sylvaticumは,オオムギの病原菌としては世界で最初の記載である。分離や接種試験の結果から,P. spinosumが最も重要な病原菌で,ついでP. ultimum var. ultimumであり,P. sylvaticumはあまり重要でないと結論した。
Spheroplasts of Plasmodiophora brassicae Woronin were successfully obtained from resting sporangia by digestion with Novozym 234 and/or chitinase. Digestion of the resting-sporangial wall by these enzymes was enhanced by a series of pretreatments with EDTA, 2-mercaptoethanol, protease and lipase. Electron microscopy of the spheroplasts showed that outer and middle wall layers of resting sporangia were removed by these pre- and post-treatments.