マツ類こぶ病菌(Cronartium quercuum Miyabe ex Shirai)の冬胞子堆形成コナラ葉片由来の培養菌株を確立するとともに,夏胞子堆形成コナラ葉片及び冬胞子堆形成コナラ葉片由来の培養菌株を無菌のコナラ葉片に接触させ,コロニーの病原性を調査した結果,夏胞子堆形成コナラ葉片由来培養菌株のみコナラ葉片上に夏胞子堆が形成された。また担子胞子由来培養菌株を無菌のアカマツ針葉及び茎組織に接触させ,宿主組織内への菌糸の侵入を観察した。
The mode of multiplication of Pseudomonas glumae on/in the rice grains inoculated at booting stage was examined. The total population of P. glumae in the grains at heading time was less than about 107CFU/g fresh weight of grain, but it increased to 108-109CFU/g during 6 days after heading. P. glumae cells densely colonized on the surface of basal part of lodicule and inner surface of lemma were observed under the scanning electron microscope. The bacterial cells invaded into interspaces of host cells of outer epidermis and spongy parenchyma of lemma were also observed in ultra-thin sections of the diseased specimen under the transmission type electron microscope.