Plant tissues, when infected by certain pathogenic fungi, seems to require an abundant supply of ATP in order to synthesize organic materials necessary in their defence reaction. Such an abundant supply of ATP may be accomplished by increased respiration of infected tissue, and the production of ATP is, as generally accepted, most efficient in case the oxidative phosphorylation takes place in the respiratory process. In fact, the respiration of the Helicobasidium-infected sweet potato tissues are stimulated up to two times, when the tissues are resistant, while up to 3 to 10 times, when the tissues are susceptible. Here arises a question as to whether the extraordinarily stimulated respiration of the susceptible tissue can be effective for ATP production. A series of experiments were made to obtain an answer to this question. The results are summarized below. Infected sweet potato tissues resistant susceptible Rate of respiration……increases up to 1.5 to 2 times increases up to 3 to 10 times Rate of triphenyltetrazoliumchloride reduction do. up to 1.5 times do. up to 3 times QO2CO2/QN2CO2…… 1.2 2.7 Total P…… increases by 15% increases by 20% Ba-insoluble P…… increases by 30% increases by 30% Ba-soluble, alcohol-insoluble.…… increases increases at first, decreases later on (Glucose-1-phosphate, Glucose-6-phosphate Fructose-6-phophate) Color development of section in molybdic-… ascorbic acid reagent visible after 2 or 3 days visible after 10 minutes Ipomeamarone (an uncoupler) in brown-discolored tissue…… +++ ++ in periphery of the above…… + ++ From these results, the writers conclude that, in the resistant tissues, the oxidative phosphorylation takes place in the stimulated respiratory process, while, in the susceptibe tissues, although the respirations are stimulated to an extraordinary extent, resultig in a rapid liberation of inorganic phosphate from phosphorylated intermediates.
In 1954 the writer reported on the quantitative survey of the free-auxin contained in various fungous galls and normal plants. The present article describes the results demonstrated with the regular Went's Avena test on the reformation and situation of free-auxin in the tissue of fungous gall as tabulated in Table 1. The results obtained are concluded as follows; The free-auxin in the young galls where the hymenium was not yet formed showed the maximum in quantity as compared with any other older galls. It gradually decreased as the gall formed the hymenium, and then became very small when the growth of gall reached at the grand period (see Table 2). As for the distribution of free-auxin in the gall, the upper part generally contained it in smaller quantity than the other parts, and the quantity gradually increased as the part of collection was situated lower. Such a fact may show that the free-auxin formed in gall migrates down toward the root pole. The longer the longitudinal length of the stem- and or leaf-gall is taken, the greater the quantity of auxin is diffused. Such an ability of migration, however, was not shown to be in exact proportion to the size of the piece of gall, and the remaining quantity of auxin in the piece increased as the size is taken larger (see Table 3 and 4). When the cut piece of gall was put upright in water in petri dish and held still in an incubator at 23°C., the contained auxin diffused off into the water in 6 hours or so, though a small quantity of it remained in the piece (see Table 5). On the contrary the negative result was shown as the diffusion was done in running water at 16-18°C. Rape plant infested with Albugo candida from which the auxin was eliminated according to the very procedure formed the auxin again abundantly in its stem tissue in the moist chamber at 23°C. in 24 hours. The same results were not obtained with the plant tissues infested with Exobasidium Camelliae var. gracilis and E. Symplocijaponicae (see Table 6). It seems to the writer, therefore, that the most part of auxin in the gall tissue are produced by these parasitic fungi themselves. Both cut terminals of gall piece of a certain length were connected to the dry cell of a definite voltage for a definite time (in minutes), and then they were cut vertically or crossways. The auxin of the cut tissues at each side of electric poles were measured. The result obtained showed that the tissue at the positive side contained the auxin slightly more in quantity than that at the negative. In all these experiments the fact that the node controls the migration of auxin was not taken in consideration.