In 2002 and 2003, sensitivity to thiophanate-methyl (TM) of Fusarium spp. and Microdochium nivale, causal agents of Fusarium head blight, isolated from wheat in Aomori Prefecture was tested in vitro. Five of 74 isolates in 2002 and 1 of 361 isolates in 2003 were identified as F. culmorum and were highly resistant to TM and had a minimum inhibitory concentration higher than 1600 ppm. In inoculation tests in 2005, TM did not control disease after when wheat plants were inoculated with TM-resistant isolates of F. culmorum. This report is the first on the occurrence of benzimidazole resistance in F. culmorum.
A fruit stain symptom occurred on mature fruits of Japanese pear in Tottori Prefecture, Japan. Two kinds of yeastlike fungi were isolated from the surface of diseased fruits. The isolates were pathogenic to immature fruits of Japanese pear cv. Gold Nijisseiki, and reisolated from the inoculated fruits. Based on morphological and physiological characteristics and partial sequence analyses of rDNA, the isolates were identified as Meira geulakonigii and Pseudozyma aphidis. Because several kinds of filamentous and yeastlike fungi have already been recorded as the causes of fruit stain of Japanese pear, we propose these yeastlike fungi as new pathogens.
A new bacterial disease, causing water-soaked, blight and rot of leaves and stems, was observed on endive, Cichorium endivia L., in Okayama Prefecture in October 2002. The bacterium isolated at high frequency from affected petioles of endive was identified as Pseudomonas cichorii on the basis of bacteriological characteristics and the 16S rDNA sequence. Healthy seedlings were inoculated with the isolated bacterium, symptoms of the disease were reproduced, and the bacterium was reisolated from the lesions. The name "bacterial rot" was proposed for the disease.