A mixture of CuSO4 and acetic acid disinfested wax gourd seeds that had been infested with Acidovorax citrulli more effectively than did ascorbic acid, peroxyacetic acid or a mixture of oxytetracycline, streptomycin and CuSO4. Seeds of melon, cucumber and wax gourd were completely disinfested after a 60-min soak in the mixture followed by dry-heat at 75°C for 4 days without affecting germination. Seeds of brown seed squash, large seed squash and bottle gourd were disinfested by a 60-min soak in the mixture followed by dry-heat at 80°C for 4 days, which caused a small delay in germination. Any treatments of small or white seeds of squash that combined a soak in the chemical mixture with dry heat and effectively disinfested the seeds also inhibited germination.
Fruit rot of a local variety of okra [Abelmoschus esculentus (L.) Moench], Shiro Okura, has been observed in Yamaguchi Prefecture, Japan since 2008. The causal bacterium was identified as Pseudomonascichorii, a pathogen causing okra bacterial leaf blight, according to its phenotypic characteristics and 16S rDNA-based phylogenetic analysis. When Shiro Okura fruits were inoculated with the isolates, the fruit rot was reproduced, and the bacterium was reisolated from the plants. To our knowledge, this is the first report of fruit rot of okra caused by P. cichorii. We propose adding fruit rot as a new symptom of okra bacterial leaf blight.