In 2006, crown rot of strawberry (Fragaria×ananassa Duchesne cv. Kentaro) was observed in production field in Hokkaido, Japan. The causal agent was identified as Colletotrichum acutatum Simmonds ex Simmonds, based on morphological characteristics, sensitivity to the fungicides benomyl and diethofencarb, and results of a polymerase chain reaction (PCR) using a specific primer. Inoculation tests with C. acutatum revealed that the fungus caused the same symptoms on strawberry plants and was reisolated from the symptoms. This is the first report of anthracnose crown rot on strawberry caused by C. acutatum in Japan.
Leaf spots and brown mottled fruit were recorded on Indian mulberry (M. citrifolia L.; Rubiaceae) in Okinawa, Japan during 2007. An Alternaria species was isolated from the diseased leaves and fruits, and typical symptoms were reproduced after inoculation of wounded or intact leaves. The causal agent was identified as Alternaria alternata (Fr.) Keissler based on morphological characters and growth temperature. This report is the first of Alternaria spot of Indian mulberry caused by A. alternata.