日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
55 巻, 7 号
選択された号の論文の27件中1~27を表示しています
  • 佐々木 浩一
    1989 年 55 巻 7 号 p. 1127-1131
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Bottom sediments of Isobe Ground in southern Sendai Bay were analysed to clarify the factors affecting living conditions of the surf clam. The sediments were mostly composed of sand, and the surf clam lived in well-sorted fine to very fine sandy bottoms with less organic matter, shallower than 12-15m deep. They were distributed in a shoaling wave zone between the breaker zone and the wave base.
    The surf clam seemed to depend on hydrodynamic conditions responsible for sediment properties rather than on the sediment itself. Since the surf clam feeds primarily on suspended rather than deposited material, bottom water movement great enough to supply suspended organic matter constantly, may be an important factor, causing the surf clam to be concentrated in the shoaling wave zone.
    Sediment with larger particles that hinders burrowing, or increased mud content causing insufficient supply of food negatively influence the distribution of the surf clam. The surf clam may also be affected by the rough waves in the surf zone.
  • 谷口 和也, 白石 一成, 蔵多 一哉, 鈴木 稔
    1989 年 55 巻 7 号 p. 1133-1137
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Effects of the methanol extracts of the brown alga Dilophus okamurai on the settlement and the metamorphosis of the abalone Haliotis discus hannai veligers were examined. Lethal effect as well as inducing abnormalities of behavior and metamorphosis were detected in the neutral fraction of the algal extract. Two spatane-type diterpene alcohols, as inhibitors for settlement and metamorphosis of the abalone veligers, were isolated from the neutral fraction.
  • 小長谷 庸夫, 蔡 慶華
    1989 年 55 巻 7 号 p. 1139-1144
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Swimming movements of silver carp Hypophthalmichthys molitrix and bighead Aristichthys nobilis were measured by means of telemetric method in summer when the water temperature was high in Lake Donghu, Wuhan, China. The positions and speeds of the fish were calculated by the differences of the travelling times of sonic pulses from the acoustic tag to three points in water. The swimming of silver carp was lower in mean speed and shorter in maximum sustained time than that of the bighead. The silver carp swam in the layer close to the surface about 16 h and the bighead surfaced only about 4.5 h in a day. The mean swimming speeds of silver and bighead carps in summer were about 10 and 29 cm/s respectively. Swimming of silver carp in summer was slower than in autumn. Swimming area of the fishes was wider in summer than in autumn.
  • 澄川 精吾, 池田 範子
    1989 年 55 巻 7 号 p. 1145-1148
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    A detailed study was made on the distribution of digestive tubules within the midgut gland, to estimate digestive phase in each specimen of the mussel Mytilus edulis.
    Of the three parts (anterior, middle and posterior) in cross sections of the midgut gland, the middle contained more numerous tubules (about 1200 tubules) than the other two (about 1000, respectively). In each section, the tubules within the right half were approximately equal in number to those in the left half and the abdominal area contained more tubules than the dorsal. The mussels examined did not show two types of synchrony (monophasic and diphasic), and all four tubule types were always observable throughout the diverticula in each specimen collected.
    Therefore, it is concluded that the samples obtained from an overall area of a right or left half in the cross section of the midgut gland are adequate than those from the dorsal or abdominal area for estimation of the digestive phase in the mussel specimen.
    The clustering of the tubules around common and neighboring secondary ducts showed similar tubule types in the histological structure.
  • 田北 徹, 斎藤 秀嗣, 大岩 厚
    1989 年 55 巻 7 号 p. 1149-1156
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Changes in size, water content and C/N ratio were examined on young Nibea albiflora distributed in the Ariake Sound to elucidate their growth and body condition which might decide the success of recruitment. The specimens were collected from the head of the Ariake Sound and the mouth area of rivers which drain to the sound. The youngs occurred twice a year, in June and August. The cohort occurring early grew fast attaining about 150mm in standard length by the end of the year, whereas the other was as small as 60-70mm. The C/N ratio decreased initially with growth, and increased afterward in fall, and the water content decreased consistently. The early changes were thought to be characteristic of young stages and the ones in fall due to the accumulation of energy to overwinter. The body component analysis revealed that the cohort occurring early stores enough energy in fall facing the first winter, whereas the other fails to do that, probably due to food scarcity in the habitat. Local populations of young N. albiflora having different growing conditions were found to exist in the sound.
  • 渡邉 翼, 森友 忠昭
    1989 年 55 巻 7 号 p. 1157-1160
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    We examined the susceptibility of Yamame embryonated liver cells, YEL-13, to Oncorhynchus masou virus, OMV. OMV did not produce syncytium on a YEL-13 cell sheet. Degeneration of YEL-13 was observed microscopically at the 9-12th day of infection and plaques were formed at the 18th day when incubated at 15°C, whereas syncytia on YNK were observed within 5 days and plaques were produced at the 7th day. The cytopathic effects (CPE) of OMV to YEL-13 were characterized by picnotic nucleus, basophilic cytoplasm, and round cell shape. At 20°C, no degeneration nor syncytium was observed both on YEL-13 and YEK cell sheet. When transferred from 20°C to 15°C, a few plaques with similar CPE to the cells incubated at 15°C from the beginning appeared in OMV-infected YEL-13 within a week. YEL-13 had about 10 times lower susceptibility to OMV and needed 2 or 3 times more time for visual plaque formation by OMV than YNK. As plaque assay of OMV in YEL-13 revealed one-hit kinetic pattern, a plaque on YEL-13 cell sheet must be initiated and produced by one particle of OMV.
  • 杜多 哲, 飯倉 敏弘, 北村 章二
    1989 年 55 巻 7 号 p. 1161-1172
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    We investigated the distribution of settled Sargassum propagules by using artificial substrata and measured water motion around a Sargassum forest in Gokasyo Bay, Mie Prefecture, Japan. The majority of measured propagules was Sargassum homeri (Turner) C. Agardh and the number of propagules settled on the artificial substrata ranged from 0 to 2197 individuals/50cm2. Numbers of propagule settled on the artificial substrata in near shore region were low, while numbers were high around an offshore Sargassum forest.
    These results indicate that wave motion effected the settlement of propagules to the artificial substrata in the following manners. In a near shore Sargassum forest where wave ray concentrates, the propagules that fell onto the substrata were carried out by wave motion. And in a near shore small bay where wave ray disperses and sea bottom is muddy, settlement of silt on the substrata inhibited the settlement of propagules. On the other hand, in offshore regions where wave motion has little effect on the settlement of propagules, distribution of propagules reflected the dispersion from offshore mother plants.
  • 杜多 哲, 飯倉 敏弘, 北村 章二
    1989 年 55 巻 7 号 p. 1173-1181
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    In order to predict the distribution of Sargassum propagules settled on sea bottom, we made field observations of water movement around the Sargassum forest in Gokasyo Bay, Mie Prefecture, Japan.
    Average vertical eddy diffusion coefficient (K2) calculated from vertical velocity was 1.3cm2/s in near shore Sargassum forest and 2.4cm2/s in offshore region. Using these values and sinking velocity of the propagutes, we solved the vertical transport equation and estimated vertical flux of propagules at sea bottom as a function of time after release.
    The variance (σ2) of horizontal displacement calculated from measured velocity showed good agreement with the power law σ2=atm. Combining the vertical flux and horizontal diffusion, we proposed a model that predicts the distribution of Sargassum propagules settled on sea bottom. The predicted distribution agreed well with actually measured distribution of propagules of S. horneri in offshore region.
  • 楠田 理一, 木村 喜洋, 浜口 昌巳
    1989 年 55 巻 7 号 p. 1183-1188
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    To clarify the immune response of yellowtail Seriola quinqueradiata which was immunized with attenuated live and fbrmalin killed Nocardia kampachi, changes in composition of peripheral and peritoneal leucocytes of yellowtail were examined after intra-peritoneal insertion of a capsule filled with the bacteria. Serum agglutination antibody titer increased(⟩1, 000)in groups immunized with either attenuated live bacteria or formalin killed bacteria. However, changes in number and composition of peripheral and peritoneal leucocytes in fish immunized with attenuated live bacteria differed significantly from changes observed in fish immunized with formalin killed bacteria and from controls. In the group immunized with attenuated live bacteria, the number of lymphocytes, monocytes and granulocytes increased in peripheral blood;peritoneal lymphocytes, macrophages and granulocytes quickly accumulated in the capsules and the bacteria were phagocytosed within 72h. It was found that the reaction of peripheral leucocytes and the accumulation of peritoneal leucocytes around the bacteria was more rapid in the group immunized with live bacteria than in the group immunized with formalin killed bacteria.
  • 平田 靖, 黒倉 寿, 笠原 正五郎
    1989 年 55 巻 7 号 p. 1189-1195
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    The role Of thyroid hormone on the development of larval and early juvenile red sea bream was studied by administrating thyroxine (T4) and thiourea (TU) as an anti-thyroidal agent. T4 treatment accelerated the appearance of black stripes on the body and the change in the habitat from the surface to the bottom of the aquarium. In contrast, TU treatment caused stasis of black stripes formation and delayaed the change in the habitat. These results indicate that thyroid hormone plays the role of an inducer in the morphological and behavioral transformation from post-larva to juvenile in red sea bream.
  • 羽曾部 正豪, 佐野 徳夫
    1989 年 55 巻 7 号 p. 1197-1201
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    A nerka herpesvirus NeVTA (Nerka virus in Towada lake, Akita and Aomori prefecture), isolated from the kokanee salmon fry Oncorhynchus nerka in 1974, was examined for cytopathic effects including nucleus inclusion body and virion morphology using the negative stain method. The chracteristic of NeVTA infection exhibited the Cowdry type A inclusion body in nuclei of RTG-2 cells at 24 hour after the infection. The morphology of virion purified with CsCl density gradient centrifugation showed typical and fine structures being envelopes, capsidc and some of microstructures. The additional characterization of NeVTA was carried out on an experimental infection against salmonid fry for the basic evaluation of pathogenicity. The results suggested that higher susceptibility could occur in landlocked salmon species such as O. nerka and O. masou, but lower in seagoing salmon species O. keta, O. gorbuscha and trout species S. gairdneri. No mortality was observed in an experiment against charr S. fontinalis.
  • 横山 芳博, 吉川 弘正, 上野 三郎, 満田 久輝
    1989 年 55 巻 7 号 p. 1203-1209
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    The efficacy of CO2 anesthesia combined with low temperature (cold-CO2-anesthesia) in long-term anesthesia supposing the transportation of live fish was evaluated using adult carp Cyprinus carpio acclimated at 23°C. In experiment I, which was done in an open system, all the carp be-came anesthetized in about 10 min with a 30-min cold treatment at 4°C and were safely maintained in an anesthetic state for the following 9.5h with the cold-CO2, treatment (14°C, Pco2 ?? 60-90mmHg). Judging from the changes in the depth of anesthesia, the optimum anesthetic condition for adult carp was Pco2 ?? 80mmHg at 14°C. In experiment II, using this condition, the carp could be safely maintained in a favorable anesthetic state for a total of 10h in a closed system.
  • 傅 恩波, 佐藤 修, 梨本 勝昭, 山本 勝太郎
    1989 年 55 巻 7 号 p. 1211-1216
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    In this experiment, we measured the fluid force on simplified models of aquaculture net cage in steady flow and wave. Then we calculated the drag coefficient and added mass coefficient from the measured fluid force. The main results are as follows:
    The added mass coefficient of models in wave was 12. The ratio of the drag force to the maximum wave force became small as the wave height-length ratio increased. However, the ratio of the mass force to the maximum wave force became large as the wave heightlength ratio increased. Therefore, we cannot disregard the mass force and consider only the drag force, when we calculate the fluid force acting on aquaculture net cage in wave.
  • 町井 紀之, 能勢 幸雄
    1989 年 55 巻 7 号 p. 1217-1221
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Principal dimensions of Peruvian purse seiner were analyzed to determine in future the purse seine size of a small wooden purse seiner. Also, the comparison between Peruvian and Japanese purse seiner was undertaken. Principal dimensions adopted are length overall (Loa; ft), breadth (B; ft), depth (D; ft), gross tonnage (GT; t) and engine horse power (HP; ps). Registered length (Lr; ft) was used to compare between Peruvian and Japanese boat. The data of 1969 for Peruvian and from 1959 to 1970 for Japanese were utilized. Principal dimensions of Peruvian wooden boat could be expressed as, Loa/B=3.42, Loa/D=7.63, GT/(Loa×B×D)=0.00724 and HP/GT=3.36. Comparing the Peruvian and Japanese wooden boat, the former has the dimen-sions of larger B and D for the same lr, larger GT for the same Lr×B×D but smaller HP for the same GT.
  • 梨本 勝昭, 平石 智徳, 小島 隆人
    1989 年 55 巻 7 号 p. 1223-1227
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    The dynamic of Japanese surf clams as they are in contact with and slide along the teeth of a hydraulic jet dredge are analyzed and the optimal curves for the teeth are discussed. Teeth angle is considered to be a function of the weight of the shell in the sand, the drag of the shell moving vertically, the drag of the shell moving horizontally, the static frictional coefficient between the shell and the teeth, shell length and buried depth. The parameters were measured under laboratory conditions, and the optimal variation in teeth angle for various shell sizes and depths was calculated at different points along the distance from the end of the teeth. The optimum teeth curves for a shell 10cm in length was obtained using these teeth angles.
  • 丹羽 栄二, 王 天鑚, 加納 哲, 中山 照雄
    1989 年 55 巻 7 号 p. 1229-1233
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    In order to investigate the role of gelling substance as water absorbent, the effect of high water-uptake hydrogels on the elasticity of kamaboko was examined. Examined hydrogels were commercial graftpolymers/graftcopolymers of 1) acrylate, 2) isobutylene and maleate, 3) starch and acrylate, 4) vinylalcohol and maleate as well as highly swellable 5) Sephadex G25 and 6) polyacrylamide. The storage modulus of kamaboko was remarkably increased by 1 and 4, somewhat increased by 2, 3 and 6, but scarcely affected by 5. The loss modulus was somewhat increased by all the high water-uptake hydrogels except 5. The breaking strength was increased by all of them. The breaking strain was remarkably increased by 2, 5 and 6, somewhat increased by 3, but scarcely affected by 1 and 4. Furthermore, expressible water of kamaboko was decreased by all of them. No correlationship was detected between their water-holding capacity and gelstrengthening action.
  • 塩見 一雄, 石川 勝, 山中 英明, 菊池 武昭
    1989 年 55 巻 7 号 p. 1235-1241
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Four serine protease inhibitors (AEPI-I, II, III and IV) were isolated from the sea anemone Actinia equina by acetone precipitation, gel filtration, ion-exchange HPLC and reversed-phase HPLC. They were all basic polypeptides with a molecular weight ranging from 6200 to 7000. The amino acid compositions of the inhibitors were very close to one another; they were characterized by the presence of 6 half-cystinyl residues but the absence of methionine and tryptophan. The inhibitors were active against serine proteases; inhibition for trypsin and α-chymotrypsin was displayed by the four inhibitors while that for kallikrein only by AEPI-I and II and that for plasmin only by AEPI-III and IV. However, none of the inhibitors showed affinity for both metalloproteases and cysteine proteases. These results suggested that the protease inhibitors can be classified into Kunitz-type inhibitors.
  • 加藤 登, 中川 則和, 照井 正三郎
    1989 年 55 巻 7 号 p. 1243-1251
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Frozen surimis made from Alaska pollack and thread-fin bream were ground with NaCl and with three different types of mixers. The efficiency of the mixers was examined by measuring the changes in temperature of salted meat paste, the salt-solubility of myofibrillar (Mf) protein along with cross-linking of myosin heavy chains (HC) in the paste, and the jelly strength of kamaboko made from the same paste.
    The highest jelly strength was obtained from salted meat paste ground at a temperature below 10°C and 23°C in the cases of Alaska pollack and thread-fin bream, respectively. As the temperature of the meat paste roses, insolubllizatton of Mf protein along with the formation of a myosin HC polymers was observed.
    From the results above-mentioned method the necessity of grinding surimi with NaCl, socalled “shiozuri”, in order to produce a favorable quality of kamaboko was revealed.
  • 西島 敏隆, 畑 幸彦
    1989 年 55 巻 7 号 p. 1253-1257
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Kinetics for the production and consumption of vitamin B12, by the natural microbial community in a coastal seawater were analyzed, and their contribution to production and consumption is discussed.
    The total consumption rate for vitamin B12 of natural bacterial and phytoplankton communities was 4.85ng/l/day in the dark and ranged from 6.10 to 6.41 ng/l/day in the light. The contribution of the bacterial community to the total light vitamin B12 consumption was about 50-59% and the remains were due to the phytoplankton community. The half-saturation constant for vitamin B12 uptake of phytoplankton (larger than 5μm) were 0.2-0.3 ng/l in the light, and those of bacteria were 0.17-0.48 ng/l. The in situ vitamin B12 uptake rate of both communities were found to he almost saturated at the naturally occurring concentration of vitamin B12.
    The gross production rate for vitamin B12 of both communities totaled 5.32ng/l/day in the dark and 6.04-6.45ng/l/day in the light, and the bacterial community contributed about 73% to the total in the dark and about 63% in the light. Thus, the total vitamin B12 gross production rate of both communities was almost equal to their total consumption rate. The bacterial community produced vitamin B12 in excess to the amount they consumed, but the phytoplankton community only produced vitamin B12 in quantities less than the amount they consumed.
    Consequently, both bacterial nad phytoplankton communities were found to contribute greatly to both production and consumption of dissolved vitamin B12 in eutrophic coastal seawaters.
  • 野田 宏行, 天野 秀臣, 荒島 幸一, 橋本 新二郎, 西沢 一俊
    1989 年 55 巻 7 号 p. 1259-1264
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Screening of antitumour activities were performed by using 56 species of marine algae (4 green, 24 brown, and 28 red algae) from the viewpoint of possible utilization for tumour preventing material. After the preoral administration of powdered 57 species of marine plants for 14 days, mice were inoculated with Ehrlich carcinoma, and breeding was continued for further 14 days. It was found that 3 green, 8 brown, 5 red algae and Z. marina show appreciable antitumour activity. Of the marine algae Scytosiphon lomentaria (Phaeophyta) showed the higherst inhibition rate (69.8%), followed by Lessonia nigrescens (60.0%, Phaeophyta), Laminaria japonica (57.6%, Phaeophyta), Porphyra yezoensis (53.2%, Rhodophyta), Eucheum gelatinae (52.1%, Rhodophyta), Enteromorpha prolifera (51.7%, Chlorophyta) and Sargassum ringgoldianum (46.5%, Phaeophyta).
    When 24 kinds of seaweed powder samples (2 green, 13 brown, and 9 red algae) were administered intraperitoneally 7 times during 21 days to mice inoculated with Meth A fibrosarcoma, 5 brown and 4 red algae showed appreciable antitumour activities. The inhibition rates were the highest in Sargassum patens (55.5%, Phaeophyta) followed by Eucheuma cottonii (39.8%, Rhodophyta), S. ringgoldianum (39.1%, Phyaeophyta), Laminaria angustata (36.6%, Phaeophyta), Ecklonia cava (35.9%, Phaeophyta) and Chondrus ocellatus (34.7%, Rhodophyta). Among the seaweed, only S. ringgoldianum and P. yezoensis were effective against both Ehrlich carcinoma and Meth A fibrosarcoma.
  • 野田 宏行, 天野 秀臣, 荒島 幸一, 橋本 新二郎, 西沢 一俊
    1989 年 55 巻 7 号 p. 1265-1271
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    For elucidation of the entity of antitumour activity of marine algae, 17 kinds of polysaccharide preparations of seaweed origin and 24 kinds of lipid fractions extracted from various seaweeds were selected as experimental material in addition to pectin. Mice implanted with Ehrlich carcinoma and Meth A fibrosarcoma were used as assay animal. Appreciable inhibition of tumour growth was found for fucoidan preparations form Undaria pinnatifida and Sargassum ringgoldianum, carrageenans and porphyran, being 51.7-54.5, 78.1, 40.1-54.0, and 45.3-58.4 %, respectively. The antitumour activities of lipid fractions against Meth A fibrosarcoma were found to be 36.1-47.1% with all the 3 fractions from S. ringgoldianum, and those of glycolipid and phospholipid fractions from L. angustata were as high as 45.9 and 58.0%, the latter of which was obtained from nearly 1/6 amount of injection. Phospholipid fraction from P. yezoensis showed very high activity such as 64.0% which was the value at the injection of nearly the same amounts as above, and it was the highest value among all the fractions tested in this work.
  • 上野 隆二, 宇野 和明, 窪田 二朗, 堀口 吉重
    1989 年 55 巻 7 号 p. 1273-1276
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    A method for determination of oxytetracycline (OTC) in fish tissues was developed using a high performance liquid chromatograph (HPLC).
    OTC was extracted from fish tissues with 5% trichloroacetic acid containing 0.5% disodium ethylenediaminetetraacetate (EDTA). The extract was centrifuged and concentrated. The concentrated solution was washed with n-hexane and passed through a Sep-pak C18 cartridge. The cartridge was pre-wetted with 5% EDTA before use. After washing the eluate was evaporated to dryness. The residue was dissolved in acetonitrile-water (3:7). The solution obtained was analysed by HPLC using M & S Pack C18 as an analytical column and methanol-acetonitrile-0.2 M oxalic acid (1:1:3:5), pH 2.0 as a mobile phase. OTC was detected at 360 nm.
    The average recoveries of OTC from muscle, serum, liver, kidney, bone, and skin of rainbow trout were 74, 82, 75, 72, 73, and 65%, respectively. Their coefficients of variation were 4.3-7.3%. The detection limits for muscle and serum were 0.05 ppm, and those for liver, kidney, bone, and skin were 0.1 ppm.
  • 内田 直行, 小島 浩一, 田中 佳代, 安斎 寛, 西出 英一
    1989 年 55 巻 7 号 p. 1277-1281
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    Eleven freshwater fishes were kept in a flow-through aquarium system (FAS) and in a closed aquarium system (CAS) after intraperitoneal injection of p, p'-DDT (DDT). Concentrations of DDT, p, p'-DDE (DDE), p, p'-DDD and p, p'-DDT-OH in the whole body and in the CAS were determined.
    Japanese barbel, Japanese dace and char rapidly converted DDT into DDE only, and the ΣDDT in the whole CAS (consisted of fish, aquarium and rearing water) were nearly equal to the original DDT, suggesting only one initial metabolic pathway (IMP) changing rapidly the DDT into DDE. In crucian carp, loach, Japanese sculpin, floating goby and catfish, slight increase of only DDE were observed, and the ΣDDT in their whole CAS almost corresponded to the DDT injected, suggesting only one IMP converting slowly from DDT to DDE. The DDT in grass carp was immediately cleared and only DDE increased without delay, and the ΣDDT in the whole CAS was about 50% of the original DDT. In bluegill, only DDE gradually increased with clearance of DDT, and the ΣDDT in the whole CAS was about 85% of the original DDT. These results suggested that grass carp had IMP converting quickly from DDT to DDE and other metabolites undetermined and that bluegill had a similar pathway, but it was slower. Japanese eel might not have clear IMP, considering the fact that no metabolite was detected and the DDT injected remained completely in the whole CAS.
  • 浜井 昌志, 吉岡 武也, 今野 久仁彦, 新井 健一
    1989 年 55 巻 7 号 p. 1283-1287
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
    We studied the structual changes of carp myosin subfragment-1 (S-i) induced by the heat treatment by monitoring several indicators. We confirmed that, upon heat treatment, the turbidity of S-1 solution increased and that the light chain (LC) dissociated from S-1 heavy chain (HC). The tryptic digestion of native carp S-1 converted HC into intramolecular three fragments and LC-1 into cleaved LC-1', which are very similar to those of rabbit S-1. However, tryptic digestion of completely inactivated carp S-1 produced neither such intramolecular fragment nor LC-l', but small pieces of fragments, suggesting the complete disordered structure of this S-1 molecule. Since the production of three fragments from partially inactivated S-1 is proportional to the remaining activity, it is concluded that these fragments are derived only from native S-1. It is also suggested that LC binding to HC in capp S-1 is weak, resulting easily in dissociation by thermal inactivation.n LC-dissociated HC may lose its native conformation, thus increasing susceptibility to tryptic attack, aggregation, and turbidity.
  • 籔 〓, 岩城 俊昭
    1989 年 55 巻 7 号 p. 1289
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 中島 謙二, 内田 有恒, 石田 祐三郎
    1989 年 55 巻 7 号 p. 1291
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 小瀧 裕一
    1989 年 55 巻 7 号 p. 1293
    発行日: 1989/07/25
    公開日: 2008/02/29
    ジャーナル フリー
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