Kinetics for the production and consumption of vitamin B
12, by the natural microbial community in a coastal seawater were analyzed, and their contribution to production and consumption is discussed.
The total consumption rate for vitamin B
12 of natural bacterial and phytoplankton communities was 4.85ng/
l/day in the dark and ranged from 6.10 to 6.41 ng/
l/day in the light. The contribution of the bacterial community to the total light vitamin B
12 consumption was about 50-59% and the remains were due to the phytoplankton community. The half-saturation constant for vitamin B
12 uptake of phytoplankton (larger than 5μm) were 0.2-0.3 ng/
l in the light, and those of bacteria were 0.17-0.48 ng/
l. The
in situ vitamin B
12 uptake rate of both communities were found to he almost saturated at the naturally occurring concentration of vitamin B
12.
The gross production rate for vitamin B
12 of both communities totaled 5.32ng/
l/day in the dark and 6.04-6.45ng/
l/day in the light, and the bacterial community contributed about 73% to the total in the dark and about 63% in the light. Thus, the total vitamin B
12 gross production rate of both communities was almost equal to their total consumption rate. The bacterial community produced vitamin B
12 in excess to the amount they consumed, but the phytoplankton community only produced vitamin B
12 in quantities less than the amount they consumed.
Consequently, both bacterial nad phytoplankton communities were found to contribute greatly to both production and consumption of dissolved vitamin B
12 in eutrophic coastal seawaters.
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