巻号一覧
56 巻, 11 号
選択された号の論文の31件中1～31を表示しています
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早川 康博, John D. Booth, 西田 周平, 関口 秀夫, 税所 俊郎, 橘高 二郎1990 年 56 巻 11 号 p. 1703-1716
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーDaily settlement of the puerulus stage of the red rock lobster Jasus edwardsii at Castlepoint in the North Island of New Zealand was monitored on artificial collectors during January and Feb-ruary 1989. Considerable differences in the average daily settlement rates were found between groups of shore collectors, and with time. Settlement rates on all collectors increased near new moon in early February, these increased rates generally persisting until the experiment ended near full moon late in February. The highest settlement rate was 5 pueruli per collector per day. Overall means of daily settlement rates of pueruli were 0.75 and 0.50 per offshore and shore collector, respectively, while very small numbers of the newly moulted post-pueruli were captured. It was not possible to relate directly changes in rates of settlement with any of the environmental variables recorded. Unlike in many other palinurids, it appears that larval recruitment rates for J. edwardsii at Castlepoint can be estimated from collectors checked at any part of the lunar cycle. It is possible that some pueruli from nearby substrata migrate onto the shore collectors. Artificial collectors such as the ones used in this study are a potential means of collecting pueruli for ongrowing in aquaculture.抄録全体を表示PDF形式でダウンロード (1277K) -
早川 康博1990 年 56 巻 11 号 p. 1717-1729
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーMean seasonal changes of dissolved inorganic nutrients (silicates, phosphates, and nitrogen compounds) in the Ofunato estuary were represented on the basis of monthly measurements from April 1979 to March 1989, and were compared with those in coastal waters. Estuarine nutrients increased in all layers in winter and spring, and decreased in the middle layer in summer and autumn, during which, however, high concentrations were observed in the surface and the bottom layer. A rapid depletion of nutrients occurred from February to March in the estuary in relation to phyto-plankton blooms, and from April to May in coastal waters.
Estuarine nutrients were greatly influenced by fluxes of nutrients from the river into the surface and from bottom sediments into the bottom, as well as fluxes from coastal waters in which the seasonal changes were mainly attributed to those in the oceanographic structure.抄録全体を表示PDF形式でダウンロード (5675K) -
廣瀬 慶二, 香川 浩彦, 吉田 勝, 熊倉 稔, 山中 英寿1990 年 56 巻 11 号 p. 1731-1734
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーA nonbiodegradable copolymer pellet of luteinizing hormone-releasing hormone (LHRH) was first examined to induce final oocyte maturation and ovulation in ayu Plecoglossus altivelis. LHRH copolymer pellet (100 or 50μg/pellet/fish) induced ovulation in all fish on 3 days following implantation and their success rates were much higher than those of pituitary homogenates used in the present study. Ultrastructural observation showed a marked degranulation in the gonadotropic cells of the fish treated with the copolymer pellet. In vitro experiment showed that copolymer pellet released native LHRH at a retatively constant rate for 75 days. These results suggest that LHRH copolymer pellet used in this study is useful for a long time treatment of LHRH to induce sexual maturation in fish.抄録全体を表示PDF形式でダウンロード (545K) -
四井 敏雄, 山田 潤一1990 年 56 巻 11 号 p. 1735-1739
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーThe zygotes grew into thick branched filaments under water temperature higher than 20°C. When the filaments were cut into small pieces with razors, they regenerated shoots and grew into new filaments. By means of cutting treatments, the filaments propagated in the culture vessels of Erlenmeyer flasks. Before cultivation, the filaments were cut into small pieces which were allowed to attach on nori-nets as seedlings according to the following procedures: the small pieces were scattered on the netting twines immersed in culture vessels fullfiled with culture medium, and kept aeration with air compressor for 2 to 3 months. The nori-nets were transplanted from laboratory culture to the sea for cultivation at Omura Bay, western Kyushu in 1980 and along Oga Peninsula, northern Honshu in 1985. Many Sphaerotrichia erect thalli appeared on the netting twines transplanted in December at Omura Bay and January at Oga Peninsula and grew to over 10cm length in 4 months at both localities.抄録全体を表示PDF形式でダウンロード (1205K) -
荒川 久幸, 松生 洽1990 年 56 巻 11 号 p. 1741-1748
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーIn order to find out any influence on the seaweed-bed exerted by sea water turbidity, the occurrence of seaweed zoospores adsorbed into suspended matters during their spent season, and the sedimentation velocity of those zoospores combined with suspended matters, and also the probability of their insertion on a base-plate were examined. In the experiment, Ecklonia cava and Undaria pinnatifida were used for the seaweeds and Kaolinite for the material of suspended matters. As a result, it had been concluded that the occurrence of zoospores adsorbed into suspended matters has nothing to do with whether they have flagella or not and the attainability of such adsorption escalates with turbidity concentration; that the sedimenta-tion velocity of zoospores themselves in the filtered sea water is in a range from 0.65 to 0.90cm but it decreases when they are adsorbed into suspended matters; that the insertion of zoospores on the base-plate is disturbed not only by their adsorption into suspended matters but also by suspended matters themselves adhered on the plate.抄録全体を表示PDF形式でダウンロード (1026K) -
中村 修, 小林 牧人, 鈴木 譲, 会田 勝美, 羽生 功1990 年 56 巻 11 号 p. 1749-1753
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーRabbit antiserum to salmon-gonadotropin (sGtH) was orally administered to goldfish. The plasma concentration of anti-sGtH rabbit IgG in goldfish was estimated by antigen-binding activity of the blood plasma to 125I labeled sGtH, and was expressed as percentage to that of original an-tiserum. After oral administration, the concentration of anti-sGtH rabbit IgG in goldfish plasma markedly increased, and reached a maximum value of 1.27% at 12 hours. This value subsequently decreased gradually to 0.57% at 72 hours. The above results demonstrate that orally administered rabbit IgG is transported from the alimentary canal to blood circulation in goldfish, preserving its antigen-binding activity. In the group of fish to which was administered 1% diluted antiserum, the value of anti-sGtH rabbit IgG concentration was approximately 1% of that of the group ad-ministered 100% antiserum. This fact suggests that protein uptake is not a reaction for excessive protein but occurs even when a small amount of protein was ingested.抄録全体を表示PDF形式でダウンロード (368K) -
藤野 和男, 荒井 克俊, 岩垂 一博, 吉田 東則, 中島 茂1990 年 56 巻 11 号 p. 1755-1763
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーSuccessful inductions of gynogenesis and of the two kinds of triploid animals of the Pacific abalone Haliotis discus hannai have been reported. After careful re-examination of various con-ditions developed before, selected were irradiation of spermatozoa by ultraviolet ray of a total dose of 1, 200erg/mm2 for genetic inactivation of paternal genomes and cold shock of zygote at 3°C for 15min, starting 32min after fertilization for retention of second polar body. A set of such treatments resulted in low survival rates of both planktonic and settled larvae but appreciable numbers of viable gynogenetic diploid, indicating 50 to 60 percentage induction of gynogenetic di-ploid among the animals survived. Simultaneous induction of some aneuploid animals was observ-ed through the analyses of isozyme genotypes. Comparison of the shell-length distributions be-tween the sib animals demonstrated that gynogenetic diploid animals revealed enlarged variances and marked deviations in mean values towards either bigger or smaller direction in comparison with those of normal diploid animals.抄録全体を表示PDF形式でダウンロード (562K) -
北野 庸介, 佐藤 要, 山根 勝行, 酒井 久治1990 年 56 巻 11 号 p. 1765-1772
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーThe fishhook eye of tuna long-line using nylon fishing monofilament was always observed to have a violent corrosion phenomenon compared with that of ordinary fishing gear.
Experiments on the sea water immersions and electric potential measurements using various anti-corrosive materials were conducted to evaluate the corrosion mechanism and resistance of the fishhook.
The results showed that the method attaching aluminum to the fishhook was proved to be most effective, even low-priced aluminum was observed to have a preventive effect on corrosion.
This method could reduce labor and expenses due to exchange and re-plating of the fishhook.抄録全体を表示PDF形式でダウンロード (1006K) -
藤岡 康弘, 伏木 省三, 田川 正朋, 小笠原 強, 平野 哲也1990 年 56 巻 11 号 p. 1773-1779
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーDownstream migratory behavior, plasma T4 concentrations and histological changes in the thyroid gland of biwa salmon underyearlings were examined during late spring and early summer for three consecutive years. The downstream migration was observed specifically when the flux of the river increased following rainfall. A close correlation was seen between the number of migrants and the rate of flux increase of the river. Plasma T4 levels of the fry caught in the river were variable, and no correlation was seen between the plasma T4 levels in the fish remaining in the river and lunar phase, rainfall, water flux or water temperature. However, significantly higher T4 concentrations were observed in the fish during downstream migration when compared with those in the fish remaining in the river and postmigrants in the lake. The mean epithelial height of the thyroid folliciles was greatest in the underyearling in May, shortly before the onset of the migratory season. The thyroidal activity of the migrants in late May and June tended to decrease and postmigrants in the lake had inactive thyroid. These results suggest that the rainfall or the associated changes in turbidity and flux is one of the initiators of the migratory behavior in biwa salmon, and also that thyroid hormone is involved directly or indirectly in downstream movement.抄録全体を表示PDF形式でダウンロード (730K) -
Marco Antonio Igarashi, 橘高 二郎, 河原 栄二郎1990 年 56 巻 11 号 p. 1781-1786
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーThe relationship between Nannochloropsis sp. and bacteria in culture water of phyllosomas has been analysed. The growth and survival rate of phyllosomas of spiny lobster Jasus edwardsii have been associated with the change of the colour in the culture water. The mortality of phyl-losomas increased as the water colour turned from green to white turbid. In contrast, it decreased when the water became transparent. The Vibrio which showed lytic action against Nannochloropsis sp. and Pseudomonas were the predominant genera isolated from transparent and green water, respectively. These bacteria were cultured separately and inoculated in the culture water of phyl-losomas to test the feasibility of using bacteria. The phyllosomas reared for 48 days at 19-21°C, being fed on Mytilus edulis. Relatively stable growth and survival rate were shown without Nan-nochloropsis sp. in the culture water.抄録全体を表示PDF形式でダウンロード (416K) -
和田 克彦, 古丸 明1990 年 56 巻 11 号 p. 1787-1790
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーBreeding experiments revealed that the white coloration of shell (prismatic layer) of the Japanese pearl oyster is inherited under the control of a recessive gene. Three matings were made: in two of them white female mated to white male in the other white female with brown (most common type) male. All the offsprings from the matings with white parents had the white coloration of prismatic layers with the few exceptional brown type which might have been introduced accidentally from other matings. Meanwhile only brown type of shells were obtained from the cross of white female×brown. The ratio of 3:1 in brown and white specimens were obtained from F2 of the last mating. These are expected ratios in crosses of brown heterozygous and white homozygous for a simple Mendelian character and it was estimated that the white coloration may be inherited under the control of recessive gene. Preliminary observation of the pearl layer (nacre) showed that the amount of yellow pigments seemed smaller in white specimens than in brown ones. The former would be useful for the production of pearls without yellow pigments that are less valuable than others although the shells were heavier in the brown specimens than in the white one.抄録全体を表示PDF形式でダウンロード (287K) -
傅 恩波, 梨本 勝昭, 山本 勝太郎1990 年 56 巻 11 号 p. 1791-1795
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーIn this study, we derived the equation of wave force acting on plane nets set by inclining to the direction of wave propagation. Then for examine if the equation is applicable, we measured the wave force acting on the plane nets set by inclining to the direction of wave propagation in a wave tank, and compared the calculated value and experimental value. The main results are as follows:
The calculated wave force and experimental wave force were similar. Therefore, may be we could use the calculated equation to calculate the wave force on plane nets set by inclining to the direction of wave propagation.抄録全体を表示PDF形式でダウンロード (301K) -
李 春雨, 五十嵐 脩蔵, 見上 隆克, 山下 成治1990 年 56 巻 11 号 p. 1797-1801
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーIn this paper, a mathematical modiel is presented to aid an understanding of the longline hook separation mechanism, and to evaluate the feasibilty of separation for hooks of different shapes and dimensions. The model is solved numerically under constraint conditions and simplifying assumptions. The numerical results indicate that hook separation becomes easier as the width-length ratio (w/l) becomes smaller. The proper rod height-hook length ratio (h/l) for an ordinary longline hook (w/l=0.4) is 0.50-0.56. This method of hook separation becomes difficult as the w/l increases beyond 0.6.抄録全体を表示PDF形式でダウンロード (488K) -
黒倉 寿, 難波 憲二, 石川 武1990 年 56 巻 11 号 p. 1803-1806
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーThe factors decreasing the fertilizing capacity of cryopreserved semen of Pacific oyster Crassostrea gigas were discussed. The survival, motility and morphological damage of the spermatozoa in cryopreserved semen were observed. However, a prominent decrease in fertilizing capacity of cryopreserved semen could not be explained by the decrease in survival and motility of the spermatozoa. The morphological damage at acrosome and the formation of sperm aggregation were recognized in cryopreserved semen through the observation under SEM.抄録全体を表示PDF形式でダウンロード (696K) -
木下 泉, 田中 克1990 年 56 巻 11 号 p. 1807-1813
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーIn Shijiki Bay of Hirado Island, diel discrete-depth horizontal tows with a larva net in May 1983 resulted in a total of 2, 350 larval and juvenile red sea bream (2.1-10.7mm SL), and 347 larval black sea bream (2.4-6.2mm SL) being collected. During the daytime, black sea bream larvae were concentrated near the surface, but red sea bream larvae near the bottom, with these tendencies becoming more evident with their growth. During the nighttime, the larvae of both species were dispersed throughout the water column. In the surf zone of the sandy-beaches located in the innermost part of the bay, collections with a small seine net in June and July 1983 gave a total of 241 late-larval and early-juvenile black sea bream (6.2-20.1mm SL), but no red sea bream. The separation of juvenile habitats between these related species seemed to be a consequence of the species-specific vertical distribution pattern differentiated during the pelagic larval period. A possible mechanism for inshore migration in the black sea bream was discussed in relation to their surface occurrence.抄録全体を表示PDF形式でダウンロード (511K) -
松田 皎, 胡 夫祥, 石沢 聡1990 年 56 巻 11 号 p. 1815-1820
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーThe hydrodynamic characteristics of a vertical V type otter board, which has been widely used recently by Japanese trawlers, were studied using a model with a dihedral angle and a sweepback angle. The model with 309cm2 wing area and 14.1% camber ratio were rigged for an attack angle ranging from -5° to 90°. The lift, drag and moment were measured by a three-component balance on a circulating water tank. Results showed that the maximum lift coefficient was 1.27 while the maximum lift-drag ratio was 4.03 when the stall angle was 22°. The range of the common attack angle sustaining 75% of maximum lift-drag ratio was comparatively wide (17°), and variation of the coefficient of pressure center was only 10%. It follows that this type of otter board is of good stability.抄録全体を表示PDF形式でダウンロード (359K) -
逸見 光, 秦 正弘, 秦 満夫1990 年 56 巻 11 号 p. 1821-1823
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーActomyosins from coho salmon, rainbow trout, stone flounder, carp, skipjack, common mackerel and jack mackerel white muscles, and coho salmon dark muscle were combined with astaxanthin or canthaxanthin. These actomyosins bound with a high affinity to astaxanthin. The actomyosins from salmonids showed a higher affinity to ketocarotenoids than those of other fishes except common mackerel. A correlation between surface hydrophobicity of actomyosins and combination of astaxanthin and/or canthaxanthin with actomyosins was observed. The selective accumulation mechanism for ketocarotenoids is discussed.抄録全体を表示PDF形式でダウンロード (222K) -
逸見 光, 秦 正弘, 竹内 昌昭1990 年 56 巻 11 号 p. 1825-1828
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーResonance Raman spectra of salmon muscles, salmon muscle water-insoluble fraction and astaxanthin or canthaxanthin recombined with salmon actomyosin were similar to those of authentic standard dissolved in organic solvents. The circular dichroism spectrum of canthaxanthin recombined with Triton X-100 treated actomyosin showed cotton effect.
These results indicate that carotenoid-protein interaction in the salmon muscle is weak, and that astaxanthin and canthaxanthin have trans configuration in vivo.抄録全体を表示PDF形式でダウンロード (298K) -
飯島 憲章, 相田 聡, 鹿山 光1990 年 56 巻 11 号 p. 1829-1837
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーAbsorption and transport of dietary oleic acid were studied in carp of summer season, treated with dorsal aorta cannulation, after force-feeding with [1-14C] oleic acid dissolved in carp body lipids. The blood plasma was collected at various intervals after feeding, and distribution of radioactivities in plasma lipid classes, lipoprotein fractions separated by polyacrylamide gel electrophoresis and high performance gel-filtration chromatography (HPGC), and lipid classes of very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL), and others fractions separated by HPGC were investigated. Radioactivity was mainly detected as free fatty acids (FFA) and partial glycerides in HDL fraction at the initial 3h after administration of labelled fatty acid. In addition, high activity was detected also in others fraction at 3h. At 6, 9 and 12h, radioactivity was located essentially in triglycerides (TG) associated mainly with HDL fraction, followed in order by LDL and VLDL fractions. However, radioactivities in VLDL and LDL fractions were disappeared and the activity was almost detected in HDL fraction at 48 and 72h after dosing.
It was recognized from these results that a small part of dietary fatty acids were direly released as FFA associated with HDL into the circulatory system; however, they were mainly synthesized to TG and secreted in the form of HDL, followed in order by LDL and VLDL.抄録全体を表示PDF形式でダウンロード (738K) -
竹内 俊郎, 鄭 寛植, 渡辺 武1990 年 56 巻 11 号 p. 1839-1845
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーIn order to examine whether or not the availability to fish of carbohydrate ingredients as an energy source is improved by the extrusion process, potato starch, corn starch, corn, rye, and wheat flour were gelatinized by an extruder. Dietary values were compared between those containing raw and extruded ingredients using fingerling rainbow trout Oncorhynchus mykiss and carp Cyprinus carpio.
In both species, the growth, feed efficiency, and protein efficiency ratio were greater in fish fed diets containing extruded ingredients than those on diets containing raw ingredients except wheat flour which showed no differences in these performances between both the ingredients. Improvement of dietary value of carbohydrates by the extrusion process was found to be mainly due to elevation of gelatinized ratios in the diet. In case of wheat flour there was no big difference in gelatinized ratio between raw and the treated materials. The apparent digestibilities of dietary protein and lipid were not influenced by inclusion of carbohydrate ingredients, but that of starch was effectively improved by the extrusion.
Thus, the availability of carbohydrate ingredients as an energy source was found to be improved by the extrusion treatment in terms of elevation of gelatinized ratio.抄録全体を表示PDF形式でダウンロード (500K) -
澤山 茂樹, 左子 芳彦, 石田 祐三郎1990 年 56 巻 11 号 p. 1847-1852
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーSexual agglutination and zygospore formation of Chlamydomonas reinhardtii, a heterothallic organism, were used for screening of mating inhibitors in culture supernatants and cell extracts of 150 bacterial strains. Both fractions of a gram-negative bacterium, strain NT4, inhibited the mating reaction. When algal vegetative cells were treated with the mating inhibitor, both sexual agglutination and zygospore formation were inhibited. However, when mature gametes were treated with the inhibitor, zygospore formation was inhibited but sexual agglutination was not. The mating inhibitor also blocked hypnozygote formation of a toxic dinoflagellate, Alexandrium catenella, at a concentration 128 times as high as that of C. reinhardtii.抄録全体を表示PDF形式でダウンロード (685K) -
西野 甫, 田中 幹雄, 横山 理雄1990 年 56 巻 11 号 p. 1853-1857
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーThe meat adhesion to casing was investigated using retort sterilized fish sausages to which different kinds of fat and starch were added. First, meat adhesion of sausages to which two kinds of fat were added separately was compared. Corn oil produced higher meat adhesion than pork fat. And in spite of kinds of fat, the degree of meat adhesion decreased with increase of additional quantity of fat. On the other hand, meat adhesion of sausages to which three kinds of starch were added separately was compared. Of these starches, potato starch gave the highest meat adhesion. And in case of corn starch, when its additional ratio to basic mixture of sausage as a hundred was 10, the degree of meat adhesion reached maximum.抄録全体を表示PDF形式でダウンロード (922K) -
天野 秀臣, 野田 宏行1990 年 56 巻 11 号 p. 1859-1864
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーTo elucidate the properties of protein in red alga Prophyra yezoensis, protoplasts having lesser amount of polysaccharides were used as a starting material. Protoplasts were isolated from Por-phyra by pre-treatment with protease followed by wall digesting enzyme mixture consisting of abalone enzyme and Macerozyme R-l0. Proteins in protoplasts were then extracted with low (I=0.05) and high (I=0.5) ionic strength phosphate buffer (pH 7.5) and 0.1N sodium hydroxide, and analyzed for protein composition, SDS-polyacrylamide gel electrophoretic pattern, and amino acid composition.
The average content of crude protein in protoplasts was 32.7%, the amount being 5% higher than that of the thallus. The major protein in the protoplasts was water-soluble protein and it amounts to 43.2% of total nitrogen. Salt-soluble protein was a minor fraction (3.1% of total nitrogen). Electrophoretic pattern of the protein fractions consisted of many bands, and that of the water-soluble protein fraction was closely similar to that of the corresponding fractions from the thallus. In contrast, the patterns of salt-soluble and alkali-soluble protein fractions were different between protoplasts and thallus. Amino acid composition, however, was similar in all protein fractions from both protoplasts and thallus. The proteins were rich in aspartic and glutamic acids, alanine and leucine, and poor in histidine and methionine.抄録全体を表示PDF形式でダウンロード (642K) -
Suda Tandavanitj, 三木 英三, 奥谷 康一1990 年 56 巻 11 号 p. 1865-1869
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーThe flow properties of the highly viscous mucopolysaccharide produced by a marine strain of Pseudomonas (No. 9-12) were investigated. The aqueous solutions of both the native and depyruvated forms of this mucopolysaccharide had yield values even at low concentrations. Flow indices for the native form increased with increasing concentrations, but remained constant for the depyruvated form at various concentrations. The structural viscosity of both the native and depyruvated forms increased with increasing concentration. The spinnability of the native form was determined to be much greater than that hyaluronic acid and potato starch. Electron micro-graphs of the gel of the native form showed microfibrils with network structures.抄録全体を表示PDF形式でダウンロード (705K) -
野中 道夫, 平田 史生, 佐伯 宏樹, 佐々木 勇人, 松川 雅仁1990 年 56 巻 11 号 p. 1871-1876
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーBy introducing underwater mincing of fresh raw material, the quality of the food stuff tentatively named highly nutritional fish meat for food stuff (HNFM) was appreciably improved. The quality of HNFM thus obtained was examined by total activity of myofibrillar Ca-ATPase and gel forming ability of it.
The quality of HNFM prepared from the minced meat in suspension (pH 7.0) as the intermediate raw material hardly changed, while such raw material was stored 2°C for 28h. However, that of HNFM prepared directly from the meat of sardine lowered rapidly during the sardine as the raw material stored at the same condition.
The first order rate constant of denaturation rate of myofibrillar Ca-ATPase indicated that myofibrillar protein in the minced meat in suspension was 10 times more stable than that in the muscle tissue of round fish. Deteriorations of gel forming ability of both HNFM from the minced meat in suspension and round fish muscle were paralleled with the denaturation of myofibrillar Ca-ATPase activity of them.抄録全体を表示PDF形式でダウンロード (441K) -
加藤 早苗, 今野 久仁彦1990 年 56 巻 11 号 p. 1877-1883
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーThe chymotryptic digestibility of carp myosin in a filamentous form was compared with that of rabbit myosin. In the presence of EDTA, α-chymotrypsin cleaves head-tail junction selectively producing myosin subfragment-1 (S-1) and rod. Ca2+ addition protected this region from cleavage with rabbit myosin, and a small amount of heavy meromyosin (HMM) was produced. However, with carp myosin, the digestion under the same condition produced not only HMM but also roughly the same amount of S-1. This suggests the weak protection of the head-tail junction by Ca-binding light chain. S-1 production in the presence of Ca2+ was observed under all the conditions we tested; pH 6.3-8.3, 0.05-0.3M KCl, and 10-20°C.
S-1 obtained by digesting in the presence of Ca2+ is indistinguishable from that obtained in EDTA medium not only in its subunit composition, but also in its biochemical properties (ATPase activity, actin binding, and thermal inactivation rate).抄録全体を表示PDF形式でダウンロード (1613K) -
今野 久仁彦, 加藤 早苗, 江湖 正育1990 年 56 巻 11 号 p. 1885-1890
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーA rapid method for isolation of myosin subfragment-1 (S-1) from carp myofibrils (Mf) was developed. For preparation of S-1, Mf were digested by α-chymotrypsin in a medium of 0.05M KCI (pH 7.0) containing lmM EDTA, cleaving at a head-tail junction of the myosin molecule. S-1 was dissociated from F-actin upon Mg-pyrophosphate addition. Crude S-1 was obtained in the supernatant after centrifugation, then purified by (NH4)2SO4 fractionation be-tween 40 and 50% saturation, and by Sephacryl S-300 gel filtration. Since the amount of S-1 produced from thermally treated Mf was proportional to the remaining Ca-ATPase activity of Mf, it was concluded that S-1 was produced only from native myosin contained in Mf. Therefore, partially denatured muscle such as frozen or stored muscle can be used as a starting material for S-1 preparation. This also suggests the possibility that the native myosin content in muscle can be estimated from the amount of S-1 produced by the chymotryptic digestion of Mf.抄録全体を表示PDF形式でダウンロード (1962K) -
菊池 弘太郎, 武田 重信, 本田 晴朗, 清野 通康1990 年 56 巻 11 号 p. 1891
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (113K) -
齋藤 洋昭, 中村 邦典1990 年 56 巻 11 号 p. 1893
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (113K) -
大黒 トシ子, 坂口 守彦1990 年 56 巻 11 号 p. 1895
発行日: 1990/11/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (110K) -
1990 年 56 巻 11 号 p. 1900
発行日: 1990年
公開日: 2008/04/21
ジャーナル フリーPDF形式でダウンロード (29K)
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