Although vitamin requirements by fresh water fish have been studied extensively by many workers, only few works have been reported on marine fishes.
We studied vitamin B
6 or C deficiency syndromes of the yellowtail
Seriola quinqueradiata TEMMINCK et SCHLEGEL by feeding the fish with diets composed of white fish meal, gluten, glucose, cod liver oil, vitamin-mixture without vitamin B
6 or C, and mineral-mixture.
Both vitamin B
6 and C deficiency symptoms appeared after about 40 days (Fig. 1 and Table 2). Characteristics of B
6 avitaminosis of the yellowtail were anorexia, retarded growth, nervous disorder, high mortality, injury at mouth and rostrum regions, exophthalmia and scoliosis (Table 2 and Figs. 1 and 2). The fish fed with a died lacking vitamin C showed loss of activity and appetite, retarded growth, change of body color, poor growth of operculum, scoliosis, amputation of strait, and hemorrhage in the laterodorsal region (Table 2 and Figs. 1 and 3).
Fat content in the liver decreased in both B
6 and C deficient fish (Table 3).
Serum protein of the yellowtail was separated into four fractions by electrophoresis on cellulose acetate membrane (Fig. 5). In the serum of the fish fed with vitamin B
6 or C deficient diet fraction I decreased but fractions III and IV increased (Table 4).
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