The vitamin requirements of rainbow trout,
Salmo gairdneri, were qualitatively examined with the fingerings.
The vitamin test diet in Table 1 was pelleted, broken into crumbles and fed. The 14 kinds of vitamin, A, D, K, B
1, B
2, B
6, B
12, C, nicotinic acid, pantothenic acid, inositol, folic acid, biotin and choline were individually eliminated from the complete diet.
Specific deficiency syndromes were observed on the following vitamins, vitamin A, K, B
1 B
2, B
6 and C, pantothenic acid, inositol, folic acid and choline as shown in Figs. 1, 2, 3 and Table 2. These syndromes coincided fairly with those reported by the previous workers.
Among the vitamins inducing no deficiency syndromes, nicotinic acid and biotin were determined to be 0.27 γ and 0.01 γ per gram of the basal diet on dry basis, but vitamin D and B
12 were not detected.
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