巻号一覧
53 巻, 3 号
選択された号の論文の27件中1～27を表示しています
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藤尾 芳久, 田中 マルシア百合子, 原 素之, 秋山 和夫1987 年 53 巻 3 号 p. 357-362
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーTo clarify the population structure of the wild haploid laver Porphyra yezoensis twelve isozyme loci were examined by starch gel electrophoresis. The allelic frequencies were calculated for 11 sites in a natural Tomarihama population. Fst value which measures genetic differentiation was 0.1252, indicating that the population structure of the wild Porphyra yezoensis has a remarkable tendency to consist of a number of patches.
The proportion of polymorphic loci varied from 0.417 to 0.917 with a mean of 0.583 and heterozygosity over 12 loci varied from 0.123 to 0.344 with a mean of 0.197. The over all mean was similar to those obtained for diploid marine molluscs in which the population structure has a tendency to split into a number of subpopulations.
Observed number of allelic combinations at one linkage group of Aat-Cat-Dia-Gpi-Mpi-6Pgd-G6pd loci was significantly lesser than the expected number. The results can be explained by clonal propagation of this haploid plant.抄録全体を表示PDF形式でダウンロード (339K) -
渡邊 精一1987 年 53 巻 3 号 p. 363-366
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe restocking effects on a discrete growth population are considered in relation to two types (quadratic and Ricker) of reproduction system. Local stability analysis and numerical calcula-tions are carried out to clarify the dynamics of the population. The stability depends on all pa-rameters employed in the model. For the quadratic model, the restocking makes the equilibrium unstable, while for the Ricker model, the restocking produces stability. When increasing rate is high, and restocking level is low, then either population destruction occurs or crash cycles emerge. Restocking produces critical effects on population growth in some cases.抄録全体を表示PDF形式でダウンロード (265K) -
藤野 裕弘, 小野 信一, 永井 彰1987 年 53 巻 3 号 p. 367-370
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーIn the present study, the uptake of rabbit's immunoglobulin against horseradish-peroxidase (HRPO) into the columnar epithelial cells in the gut of rainbow trout have been investigated.
First of all, rabbit's antiserum against HRPO was prepared with an intramuscular injection of HRPO to the rabbit.
Subsequently, the antiserum was administered into the gut of rainbow trout.
Then, the gut was fixed with periodate-lysine-paraformaldehyde (PLP) and a thin slice was made (thickness 6-8μm) with a cryostat. The histological specimens were incubated with HRPO solution so as to produce an immuno complex between rabbit IgG and HRPO, and then they were stained by Graham Karnovsky's method.
Some of these specimens were prepared for electron microscopic observations.
Results of light and electron microscopic observations suggested that the columnar epithelial cells took up animality protein, such as rabbit IgG, by pinocytosis through the pits of microvilli without digestion and the most active uptake of rabbit IgG was found in the posterior gut of ainbow trout.抄録全体を表示PDF形式でダウンロード (1333K) -
福原 修1987 年 53 巻 3 号 p. 371-379
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーMore than 2000 specimens reared in the laboratory were used to observe larval development and growth from hatching to about 10 cm long. Observations of moving time and swimming speed were made for larval and early juvenile stages. Morphological characters of fin development and pigmentation were illustrated in detail. Growth in length was expressed by the equation Y=2.83 +0.15X+0.00247X2 for first 120 days, where Y=standard length in mm and X=days after hatching. Transformation from larvae to juveniles occurred during 9.0 to 11.0 mm standard length (SL). Swimming speed ranged from about 1.0 SL/s for larval stage to 2.0-2.5 SL/s for fish at meta-morphosis. Various characters of digestive tract, squamation and pigment pattern as well as fins developed and differentiated when the larvae transformed to juveniles. The implication of or-ganogenesis, morphogenesis and behavior in the early life stages was discussed.抄録全体を表示PDF形式でダウンロード (661K) -
町井 紀之, 能勢 幸雄1987 年 53 巻 3 号 p. 381-383
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe equations of netting weight in air are very important in designing fishing gears. We developed some equations of English knot nylon netting weight in air for a single mesh (ω: weight of two legs and one knot) and for one strip of netting (W: weight of a 100 fathom length with a depth of 100 meshes), The latter derived from the equations of a single mesh. In order to determine the constants of the equations, we used the Twine Weight Table and measured different factors of netting. Weight (W) is represented by both the number of yarns (n) and mesh size (Lm), asW=C1•n+C2•(n3/2/Lm), whereC1and C2 are the constants. Using the constants determined by the measurements, ω and W can be written respectively as follows;ω=π•(D/2)2(L+9•D)•ρ and W=n{1+(√n/3•Lm)}, where D is the diameter of twine, L is the length of one leg, and ρ is the density of twine.抄録全体を表示PDF形式でダウンロード (199K) -
町井 紀之, 能勢 幸雄1987 年 53 巻 3 号 p. 385-388
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (280K) -
楠田 理一, 河原 栄二郎1987 年 53 巻 3 号 p. 389-394
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーDirect and indirect fluorescent antibody techniques were evaluated as methods for identifica-tion of yellowtail pathogens, Streptococcus sp., Pasteurella piscicida, Nocardia kampachi and Vibrio anguillarum (serotype J-O-3) in this study. Four antibody solutions were prepared against these pathogens for direct fluorescence tests. For indirect technique, four primary antibodies were obtained from rabbits immunized with formalin killed bacterial cells and the fluorescent antibody was prepared from goat immunoglobulin-G of anti-rabbit immunoglobulin-G (G-FA). The sensitivity and specificity of both techniques were examined by applying dilutions to microscope slides coated with heat-fixed antigens of eleven fish pathogenic strains, The sensitivity of the direct technique was higher than that of the indirect one. But concerning the point of specificity, the direct technique was superior than the indirect one, These results suggest that direct fluorescent antibody technique is better than indirect technique for the identification of yellowtail pathogens.抄録全体を表示PDF形式でダウンロード (429K) -
河原 栄二郎, 楠田 理一1987 年 53 巻 3 号 p. 395-399
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーDirect fluorescent antibody technique (FAT) has been carried out for diagnosis of Edwardsiella tarda, Aeromonas hydrophila and atypical Aeromonas salmonicida infections. Fluorescent antibody (FA) was prepared against E. tarda EF-l, A. hydrophila CA-1 and atypical A. salmonicida C-5. To remove cross reactivity between A. hydrophila and atypical A. salmonicida, CA-1 FA and C-5 FA were absorbed with formalin-killed cells of atypical A. salmonicida and A. hydrophila, respec-tively. To determine if cross reaction occurred, the fish pathogen bacterial suspensions were heat fixed to slides and stained with the dilute FA preparations. At field survey, the heat-fixed smears of the kidney and the head ulcerative lesion from diseased eel were stained with FA preparations. In addition, these tissue samples were inoculated on 0.5% NaCl brain heart infusion agar 10% horse serum tryptosoy agar. EF-1 FA, CA-1 FA and C-5 FA reacted only to their respective antigens by dilution or absorption. From the 79 diseased eel, the respective numbers of eel where the bacteria were detected by the method were as follows: E. tarda, 60, 51; A. hydrophila, 15, 15 and A. salmonicida, 10, 7 (bacteria, by FAT, by culture). The FAT diagnosed 8 mixed infections as compared to 5 by culture, These results indicate that FAT was superior to culture method for diagnosis of the bacterial diseases in eel.抄録全体を表示PDF形式でダウンロード (365K) -
伊丹 利明, 高橋 幸則, 佐藤 和也1987 年 53 巻 3 号 p. 401-406
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe skin mucus of ayu was collected from the body surface and its homogenate in phosphate buffer (pH 6.8) was fractionated by Toyopearl HW-50. Each fraction from the gel was examined on the bacteriolytic activities against Micrococcus lysodeikticus or Vibrio anguillarum, the etiological agent of vibriosis of ayu. The activity was determined by the turbidimetric method using the lyophilized cells of M. lisodeikticus or the formaline-killed and lyophilized cells of V. anguillarum.
The heighest bacteriolytic activities in the fractions against both M. lysodeikticus and V. anguillarum appeared in the same fraction. This fraction showed the maximal bacteriolytic ac-tivity against M. lysodeikticus in 0.01M Tris-HCl buffer of pH 7.2 at 30°C and also showed that against V. anguillarum in 0.01M Tris-HCl buffer of pH 7.7 at 15°C. These activities were partially inhibited by ρ-chloromercuribenzoic acid, but in contrast the lysozyme from hen egg-white was not. After this fraction was treated with chitin, the bacteriolytic activity against M. lysodeikticus disappeared. The optimal temperature for vibriolytic activity in the chitin-absorbed fraction shifted from 15 to 30°C and also the optimal pH shifted from 7.7 to 8.6.
These results suggest that the bacteriolytic enzymes in the skin mucus of ayu exhibit more than two, and these work cooperatively for the protection against penetration of bacteria from the water environment into the body.抄録全体を表示PDF形式でダウンロード (1079K) -
岡本 信明, 鈴木 裕之, 中瀬 清一, 佐野 徳夫1987 年 53 巻 3 号 p. 407-409
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーExperimental oral infection of rainbow trout Salmo gairdneri with multinucleate spherical bodies of Ichthyophonus hoferi cultivated was carried out. Rainbow trout orally injected with thick walled multinucleate spherical bodies cultivated in MEM containing 10% fetal bovine serum showed 100% infection and 90% cumulative mortality at 16°C water temperature for 25 days. However, those orally injected with spherical multinucleate hyphal terminal bodies cultivated in TGC containing 1% rainbow trout serum showed no infection and no cumulative mortality. The former showed the same symptoms such as darkness of the skin colour, perforation in the body surface and/or nodular white spots in several internal organs and so on as in the naturally infected fish. Amoeboblasts which underwent endogenous cytoplasmic cleavage were also observed in the liver of the rainbow trout infected artificially.抄録全体を表示PDF形式でダウンロード (1090K) -
嶋村 哲哉, 余座 和征, 長谷川 英一, 古田 昌弘, 添田 秀男1987 年 53 巻 3 号 p. 411-415
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThis survey with biotelemetry system was conducted to observe swimming behavior of chum salmon Oncorhynchus keta. Two experiments were conducted off the coast of Abashiri and Cape Notoro, Hokkaido, early in September 1984.
The results obtained are as follows:
1) The direction and speed of horizontal swimming differed between the experiments, i.e. experimental fish moved to SE-S with average speed of 0.52m/sec (0.76 FL/sec) and westward with average speed of 0.70m/sec (0.96 FL/sec).
2) The average swimming depths were 58.8m and 55.0m respectively.
3) Regarding the vertical movement, rising and diving speed of each experiment showed no notable difference.
4) Horizontal swimming was remarkably faster than vertical moving.抄録全体を表示PDF形式でダウンロード (361K) -
馬場 治, 谷内 透, 能勢 幸雄1987 年 53 巻 3 号 p. 417-424
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThree species of small deep-sea squaloid sharks, Etmopterus lucifer, E. unicolor and Centro-scyllium ritteri, were examined to study their vertical distribution and feeding habits on the basis of specimens collected from the continental shelf and upper slope off Choshi, located at the Pacific coast of Japan. E. lucifer inhabited the shallowest (mean depth range 200-300m) waters among the three species whereas E. unicolor lived in the deepest waters (mainly in 450-850m depth). E. lucifer showed an increase in depth with growth, Sex ratios by depth varied from species to species. Males always outnumbered females in E. lucifer. Stomach content analysis for E. lucifer demonstrated that small squids (mostly Watasenia scintillans) were the most important prey and that fish (mainly myctophids) were next in importance. Food items of E. unicolor con-sisted mainly of Watasenia scintillans, myctophids and Natantia (Acanthephyra sp.). Euphausiids were the main food items constituting 97% of the total number of the prey in C. ritteri. On the other hand, fishes showed high frequency of occurrence in stomach contents of C. ritteri although they were not of importance in numbers. In E. lucifer, the dietary importance of crustaceans like euphausiids decreased with size and as they grew larger, they tended to feed on squids and fishes. From the above observations food overlaps among these three species are considered to be low.抄録全体を表示PDF形式でダウンロード (673K) -
高橋 幸則, 梶脇 利彦, 伊丹 利明, 岡本 隆義1987 年 53 巻 3 号 p. 425-431
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe enzymatic properties of the bacteriolytic activity was examined on the homogenates of skin mucus in yellowtail Seriola quinqueradiata. The activity was determined by the tubidimetric method using both lyophilized cells of Micrococcus lysodeikticus and Pasteurella piscicida as the substrates.
The skin mucus showed high bacteriolytic activity against both cells in distilled water or in low molar buffer. The activity against Micrococcus lysodeikticus was maximal at pH 8.0, 30°C and that against Pasteurella piscicida was maximal at pH 8.0, 50°C. Furthermore, the skin mucus after absorption to chitin hardly showed bacteriolytic activity against Micrococcus lysodeikticus but that against Pasteurella piscicida remained. The bacteriolytic activity of the skin mucus against both cells were completely inhibited by ρ-chloromercuribenzoic acid sodium salt (ρ-CMB), and that against Pasteurella piscicida was 40% inhibited by phenylmethylsulfonyl fluoride (PMSF). Ethylenediaminetetraacetic acid (EDTA) increased the activity against Pasteurella piscicida.
From the above-mentioned properties, it was suggested that plural bacteriolytic substances existed in the skin mucus of yellowtail.抄録全体を表示PDF形式でダウンロード (539K) -
森 徹, 村中 隆司, 幹 渉, 山口 勝己, 鴻巣 章二, 渡辺 武1987 年 53 巻 3 号 p. 433-438
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーPigmentation of cultured sweet smelt Plecoglossus altivelis was attempted by using diets supplemented with a commercial preparation of Spirulina maxima, whose main carotenoid is zeaxanthin. Addition of 3 to 6% Spirulina to diets and feeding them for 10 weeks improved successfully the color of test fish. Supplementation of 4 to 8mg lutein A per 100g of diet was also tried for comparison and was found to be effective. Carotenoids in the diets were accumu-lated not only in the integuments but in the subcutaneous tissues. The contents of the integu-ments and subcutaneous tissues in the test groups were 4.2 to 5.7mg/100g and 19.5 to 44.0mg/100g, whereas those in the control group were 2.9mg/100g and 12.7mg/100g, respectively. In the integuments major carotenoids were zeaxanthin, β-cryptoxanthin, lutein B, and diatoxanthin together with minor ones such as 3'-hydroxyechinenone, cynthiaxanthin, and β-carotene triol. No lutein A was detected in the Spirulina-fed groups. In the subcutaneous tissues appreciable amounts of β-carotene and canthaxanthin were found in addition to the carotenoids mentioned above, but no lutein B was detected. Presumed metabolic pathways of carotenoids from diets to the integuments via the subcutaneous tissues were discussed.抄録全体を表示PDF形式でダウンロード (399K) -
坂本 慎一, 高田 早苗, 関 伸夫1987 年 53 巻 3 号 p. 439-444
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (2943K) -
黒島 良介1987 年 53 巻 3 号 p. 445-450
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーCadmium accumulation and its effect on calcium metabolism in girellas were studied during 24 weeks of exposure to sublethal cadmium levels (25 to 250μg Cd/l) in filtered sea water. After the 24 weeks of exposure, the fish were reared in clean sea water for 10 weeks and the removal of cadmium from the bodies was investigated.
A large amount of cadmium was accumulated in the livers and kidneys during the exposure. Cadmium levels in the gills and intestines reached to the maximum amount within 8 weeks and remained constant thereafter. Cadmium contents in the gills and intestines decreased remark-ably after the end of the exposure period. The 4 week exposure of girellas to cadmium caused a depression of the serum calcium concentrations in proportion to the cadmium levels in the test water. However, there was no difference between the serum calcium concentrations of the ex-posed fish and those of the control after 16 weeks. Calcium contents in the kidneys after the 24 week exposure showed a tendency to decrease in proportion to the cadmium levels in the test water. The exposure to cadmium had no effect on the calcium contents in the livers, intestines, gills, muscles and vertebrae.抄録全体を表示PDF形式でダウンロード (382K) -
横本 敬紀, 西田 清義1987 年 53 巻 3 号 p. 451-456
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーWhen akazara striated adductor myosin was heat-treated at various pHs, inactivation of Ca-ATPase activity proceeded more slowly at pH 7.0 to 8.0 than in higher or lower pH range. Then, heat-inactivation rates of akazara myosin were determined in 0.6M KCl at pH 7.0, using Ca-ATPase, Mg-ATPase, actin-activated Mg-ATPase and superprecipitation activities as parameters.
The results obtained showed that superprecipitation activity was most susceptible, and that the latter three activities decreased in two steps consisting of a fast and a slow change.抄録全体を表示PDF形式でダウンロード (438K) -
関 伸夫, 高田 早苗1987 年 53 巻 3 号 p. 457-463
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーCarp myosin was found to be digested easily at optimal pH of 7.0 and in the presence of >0.3mM calcium ion, once heat-treated. This process was biphasic, consisting of two apparent first-order processes with fast and slow rate constants. Both rate constants were significantly increased with increase in heating time and temperature, and consequently, with a progress in denaturation of myosin as measured by Ca- or EDTA-ATPase activity and in formation of ag-gregates.
It was also observed that a component with an Mr of 150, 000 (150K) was produced mainly from myosin heavy chain.
It was therefore suggested that the increasing susceptibility of myosin heavy chain to pro-teolysis to form the 150K component by calpain is closely related to heat-induced conformational changes of myosin.抄録全体を表示PDF形式でダウンロード (1253K) -
丸山 俊朗, 三浦 昭雄, 吉田 多摩夫1987 年 53 巻 3 号 p. 465-472
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe effects of chlorinated effluent of activated sludge treatment on the growth of Porphyra yezoensis were investigated with different chlorinated effluent-dosed culture media and different chlorine-dosed, diluted Provasoli's Enriched Seawater (PES) culture media as a control, by means of a shaking culture. To clarify the effect of the chlorinated effluent on the Porphyra thalli, the length of thalli were measured every two days, and the number of dead cells in a thallus were counted under a microscope 4-10 days after the start of the culture followed by an erythrosine staining. Initial chlorine concentrations which manifested a 50% growth effect and 50% dead cell in chlorinated effluent-dosed culture and chlorine-dosed diluted PES culture were 0.025-0.035mg Cl2/l and 0.06mg Cl2/l, and 0.75-0.95mg Cl2/l and 1.5-3mg Cl2/l, respectively. The growth effect and dead cells manifestation were dependent on the initial chlorine concentrations in both cultures. It was found by the amperometric method that chlorine was free chlorine in the diluted PES culture and combined chlorine in the chlorinated effluent-dosed culture. Although both residual chlorines disappeared with the passage of time, the removal rate of combined chlorine was slower than that of free chlorine.
The above mentioned results suggest that the strong toxicity may be caused by the reaction products of chlorine with organic substances in the effluent.抄録全体を表示PDF形式でダウンロード (689K) -
左子 芳彦, 石田 祐三郎, 西島 敏隆, 畑 幸彦1987 年 53 巻 3 号 p. 473-478
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe prospect that the cyst might be a seed population in the bloom of Peridinium penardii in spring in Nagase Reservoir, Kochi Prefecture, the conditions regulating encystment of this species were examined by use of clonal population in a synthetic medium (MW2). Sexual re-production was induced by incubating late logarithmic phase cells in nitrogen and phosphorus deficient medium. Two gametes, which were small and naked in shape, were produced from thecate cells. The gamete was isogamous and homothallic. The planozygote did not retain red bodies and remained motile for 7-14 days. About 14 days after plasmogamy the zygote became normtile, the protoplast expanded and the wall thickened. The theca of planozygote split at the girdle region and the hypnozygote (cyst) was produced. The maximum frequency of en-cystment was 40% under 10, 000lx illumination at 15°C in N-free or N-and P-free medium. The frequencies of encystment in P-free medium and fresh medium containing N and P were about 5 and 0%, respectively. Optimum condition for encystment was 15°C and 5, 000 to 10, 000lx.抄録全体を表示PDF形式でダウンロード (1096K) -
羽田野 六男, 高橋 是太郎, 座間 宏一1987 年 53 巻 3 号 p. 479-486
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーIn order to find out the most reliable index to predict the quality of the chum salmon captured during the spawning migration, measurements of body weight, body length, age, condition factor, gonadosomatic index, hepatosomatic index, proximate composition of the muscle, Hunter Lab values of meat color in relations to the organoleptic evaluation of the chum salmon dish were made using 61 fish as samples.
By observing the results obtained by principal component analysis, it was suggested that all of the biological characteristics measured do not have a direct correlation with the quality of the chum salmon as food. It was suggested by principal component analysis that the Hunter a value and b value of the meat were the most reliable index to predict the quality of the chum salmon as food.抄録全体を表示PDF形式でダウンロード (378K) -
小林 邦男, 大嶋 雄治, 田口 千恵, 汪 陽1987 年 53 巻 3 号 p. 487-491
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーA study was made of the induction of drug-metabolizing enzymes by a long-term administra-tion of PCB-diet and also the duration of their induced activities in the hepatopancreas of carp Cyprinus carpio.
In a preliminary experiment comparing the inductivity of hepatic drug-oxidase, PCB among the tested environmental contaminants was most effective as the inducer of cytochrome P-450. Therefore, a subsequent experiment was performed to elucidate the effect of long-term administra-tion of PCB (0.05mg/100g-body weight/day) for 16 weeks on drug-metabolizing enzymes in the hepatopancreas of carp. The dietary administration of PCB induced not only the cyt. P-450 content and the activities of aryl hydrocarbon hydroxylase and O-dealkylase, but also the glutathione content and the activities of glutathione- and UDPglucuronosyl-transferase. However, the in-crease of these contents and activities during PCB administration occurred mostly within 2- or 4- week period, resulting in almost no further increase in spite of the continuous PCB administration for 16 weeks. The induced contents and activities were kept for a fairly long time even after dis-continuance of PCB administration.抄録全体を表示PDF形式でダウンロード (390K) -
神谷 久男, 遠藤 良夫, 村本 光二, 内田 博之, 佐々木 琢磨, 内田 紀子, Uday Raj1987 年 53 巻 3 号 p. 493-496
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe high-molecular weight fraction from the aqueous exract of the tunicate Didemnum varians was found to have antitumor activity against Sarcoma 180 solid tumors in ICR mice, The assocaite toxicity with antitumor activity in.the extract disappeared by the pronase E digestion. The pronase digest with molecular weight over 10, 000 Da caused complete disappearance of tumor when in-jected into lesion, in the dose of 250mg/kg, The inhibition ratio observed with iv injection was less than the high values achieved by local injection, but still significant. The cytocidal ac-tivity of the pronase digest was found to reduce when compared with those of the intact specimen. Lower-molecular weight fractions which were obtained by the enzyme digestion and successive ultrafiltration gave high cytocidal activity against Sarcoma 180 tumor cells in vitro, but had only weak antitumor activity in vivo. These results suggested that the main antitumor component in D. valians was high-molecular weight and the activity was mainly due to its carbohydrate portion. The aqueous extract prepared from the different lots of D. varians showed high antitumor activity but no toxicity.抄録全体を表示PDF形式でダウンロード (299K) -
山口 宏1987 年 53 巻 3 号 p. 497-501
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe sterols of pearl-oyster Pinctada martensi were isolated by preparative HPLC, and identi-fied by GC-MS and IR. The structures of 21 sterols were established. And the sterol composi-tion was determined by using GC. The major components were Δ5-sterols. It was specially characteristic of the pearl-oyster that a lot of saturated sterols and ring-saturated sterols were also present as lesser components.
Rapid separation methods for sterols by using preparative HPLC (using a C8 column with a methanol/water (92:8) mixture as eluding solvent) was developed.抄録全体を表示PDF形式でダウンロード (295K) -
冨岡 和子, 倉賀野 妙子, 山本 裕美, 遠藤 金次1987 年 53 巻 3 号 p. 503-507
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーAt a temperature range from -5°C to 20°C, the Arrhenius plot of the dephosphorylation of nucleotides in cod muscle exhibited one break point at around -2°C, the plot of carp muscle at about 5°C, but the plot of yellowtail exhibited two break points at around -2°C and 10°C. These break points at around -2°C disappeared on the addition of glycerin as an antifreeze. The Arrhenius plots of microsomal 5'-nucleotidase of carp and yellowtail exhibited one break point at 5°C and 10°C respectively, but the plot of cod showed no break point, in the presence of glycerin. The Km of microsomal 5'-nucleotidase of carp and yellowtail for IMP markedly increased with descending temperature below the break point. The break point of microsomal 5'-nucleotidase disappeared on the treatment with a surfactant. Therefore, the assumption made is, that the change in kinetics of dephosphorylation of nucleotides at around -2°C may be accounted for by the phase change of water in fish muscle, and the change at the temperature above freezing point may be accounted for by the lipid phase separation in membranes containing 5'-nucleotidase at the definite temperature which is specific to the species of fish.抄録全体を表示PDF形式でダウンロード (404K) -
松谷 秀次郎, 瀬戸川 城将, 若宮 建昭, 中元 秀剛, 芝 哲夫1987 年 53 巻 3 号 p. 509
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (88K) -
杉田 治男, 高橋 敏文, カメモト フレッドＩ．, 出口 吉昭1987 年 53 巻 3 号 p. 511
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (111K)
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