The vitamin B
12 content of several fishes was determined by microbiological assay using
Euylena glacitis as test organism. The results are summarized in Table 4.
The values in the table are obtained by determining the B
12 content in the water extracts of the materials, and the values in gothic letter are obtained by determining the B
12 content in the water extracts of the materials which are previously digested by pancreatin.
From the data of Table 4 following facts are revealed.
1) The B
12 content of liver is higher than that of other bodily parts, and the value for ordinary flesh is low.
But time B
12 content of dark flesh is remarkably higher than that of ordinary flesh.
2) Individual fishes of same species vary considerably in B
12 potency of tissues.
3) The B
12 content of livers of gray mullet,
Mugil cephalus, and yellow tail,
Seriola qunqueradiata, is very high, and that of shark,
Carcharinus japonicus, and alaska pollack,
Theragra chalcogramma, is very low.
4) The B
12 content of flesh of pacific sanry Cololabis saira is high.
The B
12 in flesh of shark,
Carcharinus japonicus, could not he detected.
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