日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
56 巻, 1 号
選択された号の論文の29件中1~29を表示しています
  • 町井 紀之, オレゴ ・ヘンリー, 能勢 幸雄
    1990 年 56 巻 1 号 p. 1-5
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Fundamental body characteristics of Peruvian jack mackerel and approximation of girth were studied to determine the mesh size of nets in the future. On specimens collected off the coast of Callao-Peru, the girth, height and breadth at both maximum position (suffix “m”) and operculum position (suffix “o”), weight and fork length were measured. The relationships of girths (Gm, Go), heights (Hm, Ho), breadths (Bm, Bo) and weight (W) for fork length (L) were presented as Gm/L=0.511, Hm/L=0.188, Bm/L=0.125, Go/L=0.483, Ho/L=0.169, Bo/L=0.118 and W/L3=0.113×10-4. Assuming the girth as a circumference of an ellipse, approximation of girths (Gm, Go) was attempted using maximum height (Hm) and maximum breadth (Bm). The approximation obtained could be used with high precision. The body characteristics of Peruvian jack mackerel is nearly similar to that of large-size Japanese jack mackerel.
  • 町井 紀之, 能勢 幸雄
    1990 年 56 巻 1 号 p. 7-10
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Equations to determine the mesh size of purse seine were derived by applying the cumulative normal distribution as a selection ogive. Gilling of a fish in a mesh was the only consideration. Afterwards, verification of the equations derived and determination of the mesh sizes for a Peruvian jack mackerel were undertaken. The results obtained were as follows;1)The appropriate mesh size could be expressed as Mo=(K Co/2)(-3σo/Co+L) and the inappropriate mesh size as M'=a+b L, where L is the fork length, K the rate of body contraction, Co the girth/fork length ratio of operculum girth (Go), σo a standard deviation of Go, a and b are constants. 2) Equation obtained from Mo was verified, by applying the inappropriate mesh size of purse seine (M'), that it was correctly derived. 3) Mo and M' could be empirically expressed as Mo=-6.92+0.193 Li and M'=0.240+0.199Le, where Li is the minimum fork length and Le the mean fork length. The appropriate and inappropriate mesh sizes for Peruvian jack mackerel were calculated to be 43.8mm and 62.5mm, respectively for all seasons.
  • 福田 雅明
    1990 年 56 巻 1 号 p. 11-17
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    A morphometric analysis was made of myotomal musculature, together with the measure-ment of the burst swimming speeds in Pacific herring larvae and juveniles. The frequency distribution of white muscle diameter showed that mean fiber diameter increased gradually with development but new small fibers, clearly differing from primitive fibers, were added from about 20mm SL (standard length) to 30mm SL. The diameters of red muscle fibers, however, rather continuously increased with development. The logarithmic plots of cross-sectioned area of white and red muscle against the standard length showed that both relationships in white and red muscle had two regression lines with one inflection point at 30.4mm SL and at 20.1mm SL, re-spectively. The relationships between log estimated fiber numbers of red muscle against log SL had also two regression lines with one inflection point at 24.1mm, whereas those in white muscle had two inflection point at 15.4mm and at 26.9mm.
    Swimming speed increased exponentially with development, but the relation between log swimming speed (V) and log standard length (L) differed from about 28mm SL (p<0.005):V=0.63L exp (1.04) and V=1.55L exp (0.90). The length in 28mm SL was fit for the changes of morphometry mentioned above in white muscle. This suggested that the maximum swimming speed may be correlated with the development of myotomal musculature, at least in the white muscle.
  • 原 哲之
    1990 年 56 巻 1 号 p. 19-24
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    This paper treats variations in the abundance of adult yellowtail which migrate into the Sea of Japan. With catch data in some fishing grounds along the coast of the Sea of Japan, theauthor derives an index of the population size of adult yellowtail immigrating into the Sea of Japan. The index is defined as the geometric mean of catch data at five fishing grounds.
    The fluctuation of this index corresponds well to that of sea level difference between Izuhara and Pusan in the western channel of the Tsushima Strait. However it does not correspond to the fluctuation of sea level difference between Hakata and Izuhara in the eastern channel of the Tsushima Strait. On the other hand, an exponential decreasing tendency is seen in the index over theperiod of 1975 to 1986. This probably means a decrease of adult yellowtail stock around Japan in the recent 30 years.
  • 原 哲之
    1990 年 56 巻 1 号 p. 25-30
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    This paper examines mechanisms of yearly fluctuations in catch of adult yellowtail by set net fishery along the costal area of the Sea of Japan. The author studies relations between changes in position of the cold-water off the coast and fishing condition of adult yellowtail along the coast, using the hydrographic data from monthly observation in the Sea of Japan.
    In spring, abundant catch of adult yellowtail tends to occur along the coast of Yamaguchi Pref. when the position of the Shimane cold-water deflects off the coast. In winter, on the con-trary, abundant catch tends to occur when the position of the Shimane cold-water deflects on the coast. In winter, abundant catch also tends to appear along the coast of the western part of Waka-sa Bay when the position of the Wakasa cold-water deflects on the coast.
    In spring and early summer, fewer adult yellowtail migrate to the north of Noto Pen. when the cold-water deflects on Noto Pen.
  • 森山 俊介, 川内 浩司
    1990 年 56 巻 1 号 p. 31-34
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    The ability of recombinant salmon growth hormone (rsGH) to accelerate the gorwth of juvenile caho salmon Oncorhynchus kisutch and chum salmon Oncorhynchus keta by immersion was in-vestigated. Amdinistratio of rsGH by immersion at doses of 3 or 30mg/L for 60 min every 7 days resulted in a significant increase in the body length and body weight compared to control groups. The growth promoting effects of rsGH were also demonstrated in juvenile chum salmon, which were immersed in rsGH solution of 30mg/L for 30 or 60 min every 4 days. These results strongly suggest that the observed growth stimulation following immersion is due to a specific effect of GH.
  • 示野 貞夫, Driss Kheyyali, 竹田 正彦
    1990 年 56 巻 1 号 p. 35-41
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    In order to clarify the biochemical aspects of metabloic adaptation to prolonged starvation in fish, carp Cyprinus carpio were starved for 30 days in a 800l fiber reinforced plastic aquarium, and changes in hepatopancreatic enzyme activities and body composition were determined. The protein content of the serum, hepatopancreas, muscle and whole body decreased gradually over the 30 day-period of starvation, whereas, the hepatopancreas glycogen content and serum triglyceride concentration decreased rapidly during first 7 days and remained low thereafter. However, the concentration of serum glucose remained almost constant throughout the starving period, and the concentrations of serum free amino acid and free fatty acid increased gradually. The activities of hepatopancreatic glucosephosphate isomerase, glucose-6-phosphate dehydrogenase, phosphogluco-nate dehydrogenase, NAD-and NADP-malate dehydrogenases, and NAD-isocitrate dehydrogenase were significantly decreased during starvation, whereas, activities of glucose-6-phosphatase, glutamic pyruvic transaminase and glutamic oxaloacetic transaminase were similar to or higher than those in prestarvation. These data indicate that, during starvation, glycoloysis, lipogenesis and energy production markedly decrease, while gluconeogenesis and amino acid degradation remain and continue to supply glucose in the hepatopancreas. Also, during prolonged starvation, the body protein and lipid are consumed as energy sources after an exhaustion of glycogen.
  • 李 春雨, 五十嵐 脩蔵, 見上 隆克, 山下 成治
    1990 年 56 巻 1 号 p. 43-49
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    The rush and jerk motions in relation to hooking were analyzed hydrodynamically with fish behaviour restricted by a snood, in order to gain a mechanical understanding of the hooking pro-cess.
    The rush was similar to a rapid starting performance and its kinematic stages are divisible into two. At the first stage, the fish changes its form from a stretched straight to an L-(or C-) shape by moving its tail and anterior parts laterally. The second was the propulsive stage where the fish moved its tail laterally at a high speed. During this stage, the calculated mean of practical thrust was 8.1 N. If it were not for the snood, the speed and distance that would have been covered at the end of this stage would be 5.6m/s and 37cm, respectively.
    The first stage of the jerk motion was similar to the first stage of the rush. However at the sec-ond stage, the fish changed its form either by straightening out or reversing to a C-shape by moving mainly the anterior part. The length of this stroke was approximately 1/3 times the body length.
    The distance covered and the stroke are very important factors in understanding the hooking process which includes a complicated interaction between the gear and the fish. This process must be considered at the gear designing stage.
  • 川上 宏一, 楠田 理一
    1990 年 56 巻 1 号 p. 51-53
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    As it has been shown that rifampicin (RFP), streptomycin (SM) and erythromycin (EM) show high antibacterial activity against Mycobacterium sp. in vitro. The in vivo efficacies of these drugs were evaluated in experimental infection of yellowtail Seriola quinqueradiata. Fish of 100g body weight were infected by intraperitoneal injection with 1.6×106 bacteria/fish. Immediately and 24h after infection, the fish were orally administered with 0.25-25μg RFP, 1-100μg SM or 2-200μg EM mixed with minced fish meat. Survival rates higher than the control group were shown in the groups of 0.25, 2.5 and 25μg RFP, 100μg SM and 200μg EM 49 days after injection. How-ever, Mycobacterium sp. was reisolated from sampled survivors of these groups. Although the results suggest that it is not possible to fully control natural mycobacterium infections in cultured yellowtail by using these drugs, RFP is possibly an effective therapeutic agent against mycobac-terium infection, in that, the minimum dose of RFP gave a high survival rate.
  • 石田 典子
    1990 年 56 巻 1 号 p. 55-59
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Biliary metabolites of a synthetic antibacterial agent, oxolinic acid were compared in seven species of teleosts after its oral administration. The teleosts tested were rainbow trout Salmo garidneri, Japanese eel Anguilla japonica, tilapia Tilapia mossambica, red sea bream Pagrus major, Japanese mackerel Trachurus japonicus, yellowtail Seriola quinqueradiata, and Japanese flounder Paralichthys olivaceus.
    Biliary metabolites were separated into unconjugated (FRACTION A) and conjugated fractions (FRACTION B) by extracting the biles with chloroform, the metabolites of each fraction were identified by thin-layer chromatography with the authentic samples.
    In the unconjugated fractions, only intact oxolinic acid was detected in all the species. However, in the conjugated fractions, there were some differences between the results for the freshwater fish (rainbow trout, Japanese eel, tilapia) and these for the seawater fish (red sea bream, Japanese mackerel, yellowtail, Japanese flounder). Three glucuronides of oxolinic acid, 7-hydroxy-6-methoxy derivative, and 6-hydroxy-7-methoxy derivative were detected in the bile of the former and only two glucuronides of 7-hydroxy-6-methoxy derivative and 6-hydroxy-7-methoxy derivative were detected in that of the latter.
  • 最上 和江, 北沢 智彦, 菊池 武昭, 渡辺 悦生
    1990 年 56 巻 1 号 p. 61-65
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    A sensor for the determination of fluorine was constructed using an Ag-electrode and the exoskeleton of Antarctic krill from which endogenous fluorine had been previously removed. The principle of the proposed sensor is based on the membrane potential difference being pro-duced by the recombination of fluorine on the exoskeleton of Antarctic krill. A linear relationship was observed between the rate of the increase in the output of the sensor and the concentration of fluorine (0.5-5ppm) when the sample solution was stirred at 1000rpm and 25°C. Fluorine contents in raw and dried leaves of Japanese teas were determined by the proposed sensor and a conventional method. Good comparative results were obtained between the values determined by both methods. One assay could be completed within about 30min.
  • 野中 道夫, 平田 史生, 佐伯 宏樹, 中村 誠, 笹本 泰彦
    1990 年 56 巻 1 号 p. 67-72
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    The quantity of volatile compounds (VC) arisen from the highly nutritional fish meat for food stuff (HNFM) and the intensity of the odor were compared with those of sardine surimi. VC arisen within 15min at 40°C were trapped with U-tube cooled by liquid oxygen and measured by gas-liquid chromatography. The intensity of odor was determined by a sensory test.
    The chief components of VC were found to be aldehydes and ethyl alcohol. The intensity of odor was strongly related to the contents of aldehydes in VC. The amount of aldehydes generated in VC from HNFM were 50-70% smaller than that from surimi. Moreover, HNFM which was heated at 90°C for 30min did not give any fishy odor. On the contrary, the undesirable odor of surimi became quite strong after heating.
    These results suggested that the removal of lipid in HNFM processing was very important in prevention of generation of undesirable odor from sardine meat products.
  • 安斎 寛, 内田 直行, 西出 英一
    1990 年 56 巻 1 号 p. 73-81
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Decomposition of alginate under the hydrolysis procedure with sulfuric acid was evaluated by determining the recovery of monouronates produced and its depolymerization extent. The first three hour hydrolysis with 80% sulfuric acid at 30°C, followed by the two hour hydrolysis with 2 N sulfuric acid at 100°C, resulted in complete hydrolysis with the highest recovery in the conditions examined. Under these conditions, the recovery of authentic uronates was 80.9% for D-man-nuronic acid and 62.8% for L-guluronic acid. Prolonged treatment resulted in a lower recovery of monouronates, and showed indications of an increase in the degradation of monouronates. The degradation products showing reducing power, were detectable with the phenol-sulfuric acid method but undetectable with the carbazole-sulfuric acid method. D-Mannuronic acid to L-guluronic acid ratios (M/G ratio) of nine species of alginates determined by the improved procedure were compared to those determined by the method of Haug and Larsen. The values of M/G ratio obtained by both methods were nearly equal in six species and were slightly different among three specíes. The modified method, however, showed a 9∼17% higher recovery of monouronates than the method of Haug and Larsen. In addition, the time for the completion of the analysis process was shortened by at least 1/5.
  • Mohamed Kamal, 渡部 終五, 橋本 周久
    1990 年 56 巻 1 号 p. 83-91
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    The effect of pH on the denaturation profile of sardine ordinary and dark muscle myofibrils under various storage conditions was investigated by measuring Mg2+-ATPase activity and solubili-ty. Storage at O°C for 2 days gave rise to two activity maxima both in the presence and absence of Ca2+, one at pH azound 7.5 and another at pH 9.5, and the highest Ca-sensitivity at pH 7.5regardless of muscle type. After 2 days of storage at-20°C, the remaining activities were maxi-mal at pH around 9.5 either in the presence or absence of Ca2+, while extremely low at pH between 5.5 and 7.5 for both myofibrils. When myofibrils were incubated at 35°C for 30min, Mg2+-ATPase activities in the presence and absence of Ca2+were both increased with the maximum values at pH 7 for ordinary muscle and at pH 7.5 for dark muscle myofibrils. Ca-sensitivity of ordinary musclemyofibrils almost disappeared while that of dark muscle to some extent remained, especially in alkaline pH range.
    There was no marked change in solubility and SDS-PAGE pattern of soluble protein fraction throughout pH examined with both myofibrils stored either at 0 or-20°C. The solubility of ordinary muscle myofibrils decreased below 40%at any pH after incubation at 35°C whereas SDS-PAGE pattern showed no myosin heavy chain in the soluble protein fraction. The solubility of dark muscle myofibrils also decreased considerably, especially in an alkaline pH range. This well corresponded to the SDS-PAGE pattern of the soluble protein fraction where less amount of myosin heavy chain was observed at pH above 7.5.
  • 岩本 宗昭, 山中 英明, 阿部 宏喜, 渡部 終五, 橋本 周久
    1990 年 56 巻 1 号 p. 93-99
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Yellowtail, bartailed flathead and Japanese striped knifejaw were spiked at the brain, and stored at O°and 10°C. Yellowtail set in rigor-mortis after 2h, irrespective of storage temperature, attaining the full-rigor state after 7h at O°C and 15h at 10°C. Rigor-mortis of bartailed flathead started after 10h at O°C and 13h at 10°C. This species reached the full-rigor state after 20h at O°C and 40h at 10°C. In the case of Japanese striped knifejaw, rigor-mortis began to appear immediately after killing at O°C whereas after 4h at 10°C. The full-rigor state was attained with this fish after 13 and 20h, respectively, in the above order of storage temperature. Therefore, rigor-mortis proceeded roughly two times slower at 10°C than at O°C, regardless of fish species.
    In accordance with rigor-mortis progress, the muscle of each species showed a 2-3 times slower decrease of ATP concentration when stored at 10°C than when stored at O°C. Disappearance of creatine phosphate preceded that of ATP while lactate accumulation was nearly maximal when ATP disappeared, irrespective of fish species and storage temperature. No marked differences in initial ATP concentration were observed among the fish species examined. The highest lactate level was encountered in yellowtail which exhibited the fastest progress of rigor-mortis at either storage temperature. Japanese striped knifejaw showed a much lower accumulation of lactate than yellowtail, the accumulation of which was even lower than that in bartailed flathead. On the other hand, rigormortis progress of Japanese striped knifejaw was comparable to that of yellowtail and somewhat faster than that of bartailed flathead.
  • 岩本 宗昭, 山中 英明, 渡部 終五, 橋本 周久
    1990 年 56 巻 1 号 p. 101-104
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Wild and cultured plaices were compared in terms of rigor-mortis progress during storage at 0° and 10°C. When spiked at the brain, both wild and cultured plaices exhibited the rigor-mortisonset after 3h at 0°C and after 6h at 10°C. They attained the full-rigor state after 21h at 0°C. At 10°C, wild plaice was relatively slower in rigor-mortis progress: It attained the full-rigor state after 32h while the cultured one had not yet done so. When killed while struggling and stored at 10°C, both types of fish set on rigor-mortis after 2h and attained the full-rigor state after 8h.
    In the muscle, ATP degradation and lactate accumulation proceeded essentially in parallel to rigor-mortis progress. When spiked and stored at 0°C, both types of plaice showed a decrease of muscle ATP concentration from 6-7μmol/g at the start to less than 1μmol/g after 15-18h, the time when the lactate level reached the maximum of 35-40μmol/g. When stored at 10°C, both plaices showed corresponding levels of both compounds after 32h. When killed while struggling and stored at 10°C, both types of plaice showed a low level of ATP even immediately after death, the level further decreasing to less than 1μmol/g after 2-4h. They attained the maximum lactate accumulation of 35-40μmol/g after 6h.
    It was concluded that rigor-mortis of plaice, either wild or cultured, was accelerated by both ice storage and by the death while struggling.
  • 熊沢 義之, 大嵜 良孝, 岩見 史郎, 松本 行司, 新井 健一
    1990 年 56 巻 1 号 p. 105-113
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    The effect of addition of inorganic pyrophosphate and sorbitol on freeze denaturation of myofibrillar protein was studied. Carp myofibrils suspension was mixed with sodium pyrophosphate(PPi-Na)or neutralized pyrophosphate (PPi) and subjected to storage at -20°C. The effect of increases in pH or in ionic strength induced by the additives was seaparately ex-amined by measuring the first order rate constant (KD) for freeze-inactivation of myofibrillar Ca-ATPase. The effect of further addition of sorbitol to the myofibrils suspension containing PPi-Na was also examined in the same manner. The results obtained were as follows: (1) The KD increased generally with increasing the ionic strength of the medium for freezing, although theincrease in KD brought about by PPi was somewhat larger than that by KCl. (2) The KD values reached to identical minimum at around pH 7.5, for media adjusted by either PPi-Na or Trismaleate buffer. (3) When the pH of myofibrils suspension was set at 7.5-8.0 by PPi-Na, the protecting effect of sorbitol against the freeze denaturation of myofibrillar protein was greatly enhanced.
  • 松野 隆男, 山下 栄次
    1990 年 56 巻 1 号 p. 115-118
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Two new carotenoids, (3'R, 6'S)-β, ε-caroten-3'-ol and (3'S, 6'S)-β, ε-caroten-3'-ol have been isolated from the integuments of the black bass Micropterus salmoides.
  • 塩見 一雄, 植松 英夫, 伊藤 洋, 山中 英明, 菊池 武昭
    1990 年 56 巻 1 号 p. 119-123
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    A lectin in the skin muscus of the dragonet Repomucenus richardsonii was purified to an electrophoretically pure state by affinity chromatography on Sepharose 4B and HPLC on Chemcosorb 300-7CM. The purified lectin was an acidic glycoprotein with an isoelectric point of 6.1. Its molecular weigh was determined to be 48000 by HPLC on TSK-gel G3000SW or 12000 by SDS electrophoresis, suggesting that it exists as a tetramer of the same subunit. The amino acid composition was characterized by the abundance of glycine, acidic amino acids and hydroxy amino acids and the absence of sulfur-containing amino acids. Inhibition tests revealed that the purified lectin has high affinity for D-galactose related sugars, especially lactose, and two glycoproteins(asialofetuin and mucin).
  • 関 伸夫, 宇野 秀樹, 李 南赫, 木村 郁夫, 豊田 恭平, 藤田 孝夫, 新井 健一
    1990 年 56 巻 1 号 p. 125-132
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    When carp myosin B was incubated at 25°C, SDS-PAGE showed that an addition of a soluble extract from Alaska pollack muscle and surimi greatly stimulated the cross-linking reac-tion of myosin heavy chains. This effect was completely lost on boiling but not with dialysis, suggesting the presence of an active enzyme, transglutaminase, in the extract. It was, therefore, purified from Alaska pollack muscle by DEAE-cellulose chromatography and Sephadex G-200 gel filtration.
    The partially purified enzyme catalyzed the cross-linking reaction of myosin heavy chain and also showed an activity to incorporate a fluorescent amine, monodansylcadaverine, into acety-lated casein. SDS-PAGE analysis of carp myosin B incubated with the enzyme in the presence of monodansylcadaverine showed several fluorescent bands, including myosin heavy chain and its polymers, with a depletion of fluorescence on actin, tropomyosin and connectin bands. The transglutaminase activity was found at a molecular weight of about 85, 000 by the gel filtration and required Ca2+.
    From these results, the presence of transglutaminase was ascertained in Alaska pollack muscle and surimi. The setting of the salted paste of muscle or surimi may thus involve at least the enzymatic process in gel network formation.
  • 舩津 保浩, 橋本 昭彦, 今野 久仁彦, 新井 健一
    1990 年 56 巻 1 号 p. 133-137
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Denaturations of carp myofibrils and myosin induced by changing pH and KCl concentration were studied. Myofibrils and myosin suspension was mixed with maleate-NaOH buffer to set the pH between 4.81 and 6.14 in the presence of various concentrations of KCl (0.1-1.0M) and stored at 2°C. The first order rate constant (KD) for inactivation of Ca-ATPase caused by the above treatment was then estimated.
    It was thus found that the KD of myofibrillar Ca-ATPase increased with lowering the pH as well as with increasing KCl concentration. On the other hand, the KD of myosin Ca-ATPase remained practically unchanged with changing the KCl concentration but increased with lowering the pH, in the same manner as in the case of myofibrils, In addition, the KD of myosin Ca-ATPase agrecd closely with that of myofibrillar Ca-ATPase in the presence of 1.0M KCl. These results strongly suggested that the actin part of carp myofibrils was denatured by acid treatment in the presence of 1.0M KCl, and lost its protective activity against thermal inactivation of myosin Ca-ATPase.
  • 赤羽 義章, 志水 寛
    1990 年 56 巻 1 号 p. 139-146
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Existing states of water in salt-ground surimi and its kamaboko gel were investigated in rela-tion to gel-strength and surface hydrophobicity. The salt-ground surimi of Alaska pollack was incubated at 0, 10, and 30°C for a fixed time and then heated at 90°C to produce the final gel, kama-boko. The gel-strength of kamaboko increased more or less by the incubation before heating. During storage in a vacuum-packed condition the water-release from kamaboko produced with incubation was more remarkable than that without incubation. The amounts of absorbable from the incubated surimi and its kamaboko gel into a filter paper were more than those from the nonincubated. Also, more amounts of easily mobile water were determined by the expressing method existed in the incubated surimi and its kamaboko gel. Surface hydrophobicity of the surimi measured for relative fluorescence intensity increased during the incubation at 10°C and 30°C and the subsequent heating at 90°C. This increase in the surface hydrophobicity during incubation is considered to have a close relation to an increase of easily mobile water content in the in-cubated salt-ground surimi and its heated gel, “suwari kamaboko”.
  • 濱 洋一郎, 前田 浩幸, 中村 孝
    1990 年 56 巻 1 号 p. 147-152
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Conjugated carbonyls with a hydroxypentanone ring produced during autoxidation of methyl linolenate, methyl γ-linolenate, methyl arachidonate, ethyl eicosapentaenoate, methyl docosahexa-enoate and triglycerides of squid liver oil, were determined quantitatively using gas chromato-graphy-selected ion monitoring. A fraction containing the carbonyls was separated on a Sephadex LH-20 column connected with a high performance liquid chromatograph system. After NaBH4- reduction and subsequent trimethylsilylation, peak areas of a characteristic ion (m/z 167) of the carbonyls and a prominent ion (m/z 187) of the internal standard, 12-hydroxy methyl stearate, were measured quantitatively. Production of the carbonyls during autoxidation showed a good correlation with the degree of red coloration with glycine (A515nm) of the oxidized lipids. A maximum formation of the carbonyls at 30°C was estimated to be 0.04 to 0.11%.
    In addition to the carbonyls, much larger amounts of prostaglandin-like substances, precursors of the carbonyls, were observed in the relatively early stage of autoxidation.
  • 太田 亨, 高木 徹
    1990 年 56 巻 1 号 p. 153-157
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
    Lipid class and fatty acid compositions of the testis lipids of chum salmon Oncorhynchus keta having different maturities [maturing (A), matured (B) and fully matured (C) stages] were examined with particular reference to furan fatty acids (F acids).
    There were little changes in the lipid content with about 2% during maturation. The sterol esters (SE) and triacylglycerols (TG) in the testis lipids decreased. On the contrary, the free sterols (ST) tended to increase during maturation.
    F acids were found in the SE, TG and free fatty acids (FFA), but not in the phospholipids.
    The TG contained large amounts of F acids compared with other lipid classes. The F acids of the TG increased with maturation and reached to 44.8% of the total fatty acids at the fully matured stage. On the contrary, those of the SE and FFA decreased from 18.5 to 6.5% and from 9.9 to 4.6%, respectively.
    The results in this study suggested that the changes in the F acids of the lipid classes of chum salmon testes during maturation were due to a decline in the lipid metabolism arising from the physiological fluctuation in chum salmon at late spawning season.
  • 石田 典子
    1990 年 56 巻 1 号 p. 159
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 藤井 建夫, 林 正明, 奥積 昌世
    1990 年 56 巻 1 号 p. 161
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 福田 雅明
    1990 年 56 巻 1 号 p. 163
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 田中 祐志
    1990 年 56 巻 1 号 p. 165
    発行日: 1990/01/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 1990 年 56 巻 1 号 p. 172
    発行日: 1990年
    公開日: 2008/04/21
    ジャーナル フリー
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