The organisms responsible for peculiar types of deterioration of fish sausage were found to have high resistance to nitrofurazone, nitrofurylacrylic amide and sorbic acid which are permitted to use as food preservatives in fish sausage in Japan
1).
In the present studies, lethal toxicity of the 12 strains of organisms consisting of several species of responsible organisms for various types of deterioration of fish sausage to mice by intraperitoneal injection has been determined, in addition, effect of temperature on the growth of these organisms, and their nutritional requirements for amino acids and vitamines have been examined.
Results obtained may be summarized as follows:
1. No lethal toxicity to mice by intraperitoneal injection was observed among 12 strains of the causative organisms (Table 1).
2. Four strains among 6 of
B. coagulans, responsible for the formation of spots on the surface of fish sausage, grew in the range of 30°C. and 55°C., while the remaining 2 strains grew even at 60°C. (Table 2).
The optimal temperature for growth was 45°C.
After being subcultured at 60°C., certain strains of
B. coagulans tended to become filamentous form and to lose spore forming ability. However, sporulation of the organisms and the shape of the cells were gradually recovered during incubation of the organisms at 45°C.
Growth of other causative organisms than spots-forming
B. coagulans was observed to occur in the range of 25°C. and 45°C. (Table 2).
3. Experiments conducted on the nutritional requirements by 6 strains of
B. coagulans, for 10 of amino acids and 6 of vitamines at 37°C, 45°C. or 55°C. revealed that the higher the incubation temperature, the less nutritional elements were required. However, there was no appreciable difference in the requirements between the incubation temperature at 45°C. and 55°C. (Table 4).
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