Ayu
Plecoglossus alaivelis were immersed for 3 min in a 5% NaCl solution containing 1% lyophilized formalin-killed
Vibrio anguillarurm. Two months after vaccination, each of the four experimental groups vaccinated fish were infected by intraperitoneal (i.p) injection of either 10
3, 10
4, or 10
6 cells of
V. anguillarum PT479 streptomycin resistant (Sm
r)-strain or exposed to 10
6 cells/ml of PT479 Sm
r strain. The time period of elimination of the infecting organism depended on the number of cells of
V. anguillarum given to the fish. The challenge organisms wera virtually eliminated within 6h after i.p. injection of 10
3 or 10
4 PT479 Sm
r cells in the blood and intestinal tracts. On the other hand, organisms increased 12h after injection of 10
3 or 10
4 cells/ml in non-vaccinated fish, reaching 10
6 cells/ml of blood and 10
5 cells/g in the intestinal tracts by 72h. The challenge organisms were observed in the blood and intestinal tracts of vaccinated fish Within 24h after i.p. injection of 10
6 cells. Subscquentry, the organisms decreased to 10 cells or less. The number of organisms increased in non-vaccinated fish after i.p. injection of 10
6 cells.
The number of challenge organisms was 10 cells/ml or g, or less, in the blood and the intestinal tracts of vaccinated fish 72h after exposure to 10
6 cells. The challenge organisms were also ob-served in the blood of non-vaccinated fish for 72h.
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