The percentage of carotene to the total vitamin A taken by Japanese people, is reported about 95%. It is very important problem, therefore, how carotene is utilized in human bodies. In various investigations, the carotene level in blood has been of much interest in the stage of carotene absorption in its metabolic process. In this experiment, the petroleum ether extracts gained from serum of cattle and human being were isolated by chromatography with alimina and investigated. In case of serum of cattle, β-carotene occupied almost 90% of the pigments in the petroleum ether extracts, while in case of human blood, it was only from 20 to 30% of the carotenoids pigments as shown in the table 2. Vitamin A and carotene contents of serum were estimated by Bessey's method for 47 healthy men aged from 20 to 30 year vitamin A contents gained from about 80% of total subjects were 10-39.9γ/dl, on the other hand, carotene contents gained from 30% of the total were 30-39.9γ/dl, and the average values were 25.5±11.3γ/dl and 56.3±27.4γ/dl respectively. This results showed that the vitamin A level of serum was some what low, and carotene level is distributed in more wide range than the vitamin A which held almost constant value.
The stability of mixture calcium carbonate and thiamine hydrochloride was tested. When they were kept at high humidity, thiamine decomposed very easily. At the same condition, thiamine mononitrate was stable, and the mixture of these vitamins and calcium phosphate were also less decomposed.
The stability of thiamine mixed with calcium carbonate was tested. As thiamine salt, thiamine saccharinate, thiamine phenolphthalinate, thiamine rhodanate and combined thiamine. The stability of these salts were almost same as thiamine mononitrate
This study was projected for the purpose of obtaining the rate of utilization of alcohol's calories for daily muscular work. As the first step of this study, an experiment on the metabolism of alcohol was carried out. Methods: As subjects, two males were selected as the middle or heavy drinker group (not alcoholics) and three males as the light drinker group. The experiment was made at fasting condition in the morning. The basal metabolism was determined at first, and then whisky containing 29.5g% of absolute alcohol was administered orally for a short time (two to ten minutes), being diluted to about 10% alcohol solution with water. Immediately after this the subject was lain in bed during the measurement-period of five hours. During this time the energy metabolism, pulse rate, body temperature and blood pressure were determined in every thirty minutes. The blood and urine were taken at intervals of half or an hour over eight or ten hours and these samples were analysed for alcohol concentration. The administration of alcohol were prescribed in dosages of 1.0g and 0.5g per kg body weight as absolute alcohol. The determination of alcohol was made on the basis of Gibson and Blonder's method. The results were summarized as follows: (1) The concentration of blood alcohol rised rapidly to a peak at thirty or sixty minutes after ingestion of alcohol in three subjects except one (subj. No. 2)in which a high concentration level was maintained constantly for about three hours after drinking and after that time the concentration curve of blood alcohol declined generally in a linear fashion. However, the concentration curve in 0.5g/kg alcohol intake appeared to be somewhat hyperbolic. (2) The “β” factor was higher in the heavy drinker group with an average of 18mg% per hour than in the light drinker group with an average of 14mg% per hour, in case of 1.0g/kg alcohol intake. When a dosage of 0.5g/kg alcohol was administered to the light drinker group the “β” factor was 9mg% per hour in average, and this value was lower than that of the same individual in case of 1.0g/kg alcohol intake. The “γ” factor was in the range of 0.66 to 0.78 (mean 0.71) and this factor was found to be fairly constant in the same individual and to vary from individual to individual. “ED” value (ehtanol disappearance from blood, β×γ×body weight) in case of 1.0g/kg alcohol intake was higher in the heavy drinker group with an average of 7.5g (125mg per weight kg) per hour than the value of 5.8g (95mg per weight kg) observed in the light drinker group. In 0.5g/kg alcohol intake (in the light drinker group) this value was 4.0g (65mg per weight kg) per hour and this was about 30% lower than that of the same individual in the dosage of 1.0g/kg alcohol. (3) The concentration of alcohol in urine declined in a linear form independently of the volume of urine being in agreement with the change of blood alcohol concentration. It seems that the ingested alcohol will be distributed equally throughout the body fluid after about an hour. However, the absolute amount of alcohol excretion per hour was influenced by the volume of urine. The maximum level of the hourly excretion of urine was seen at the initiatory period (within two hours after drinking) for three subjects, but for the other one (subject No. 2) was seen a peculiar type, i. e., the maximum level was at four or five hours after drinking. (4) The heat production after drinking of 1.0g/kg alcohol showed a rise of only 4% above basal metabolism in a heavy drinker (subj. No. 2) and 13 to 14% in the light drinker group. In a smallest drinker (subj. No. 5) the metabolic rise reached up to 22% markedly in 0.5g/kg alcohol intake. Thus, the metabolic rise related to individual sensibility towards the pharmacological action of alcohol. This fact was also considered validly from the results that the degree of variations in pul
In previous studies it was founded that in spite of the high content of essential amino acids except methionine in Chlorella and Scenedesmus, the algaes were not suitable for practical use on cooking as a protein source due to their special smell and taste. This experiment was designed to observe the effect of dried Scenedesmus on the growth of the albino rat. As can be seen from the results, the growth of Scenedesmus group was inferior to casein group, and then the effects of supplementation of methionine and decolored Scenedesmus on the growth of this group were observed for 2 weeks. The protein of decolored Scenedesmus was found more effective on the growth of rats, but the supplementation of methionine not so effective.
In continuation to the previous report, the effect of decolored Scenedesmus on the growth of albino rats was observed again, but this time for 40 days long. The results indicated that there is no significant difference between those effects on the growth and their protein effciencies.
This experiment was designed to observe the effect of supplementation of methionine to decolored Scenedesmus on the weight gain and liver nitrogen of albino rats by the depletion method. It is recognized from the results that the effects of supplementation of methionine on the weight gain and total liver nitrogen of animals are a little greater than those of Scenedesmus group.