It has been already reported by us that the growth of the implanted tumor is remarkablly affected by the restriction of essential amino acids. From our amino acid imbalance studies, it has been found that the restriction of phenylalanine causes the returdation of tumor without any undesireble side effect against the host. Such effect of phenylalanine deficient diet has also been found to be accelerated by the restriction of tyrosine. The phenylalanine hydroxylase activity of liver decreased remarkablly in the tumor bearing animal. Foretheremore, it has also been confirmed that the restriction of an essential amino acid causes the elongation of the doubling time of tumor cell. From the experimental facts mentioned above, the effectiveness of amino acid imbalance diet has been clinically investigated in all types of leukemia and other malignant diseases. The patients have been treated initially with low phenylalanine diet and then recently with low phenylalanine-tyrosine diet. The combined restriction therapy of phenylalanine and tyrosine seemed to be more effective than the single restriction of phenylalanine. Phenylalanine-tyrosine limiting milk according to our prescription has been used as the main nitrogen source of the diet. The dosis of phenylalanine and of tyrosine supplied daily were 1.8g. respectively for adult and were 1.1g. for infant. Such phenylalanine dosis as mentioned above exceeds slightly the lowest essential dosis of it. Up to now, 22 patients were treated with this amino acid imbalance diet for one month to tow and half years, without any side effect. The patients treated with this diet were as follows; 11 cases of acute leukemia including 5 infants, 6 cases of chronic leukemia and 5 cases having solid malignant tumor. Single application of this diet not so effective except few successful cases. The clinical responce against chemotherapy, however, was remarkably improved with this diet therapy and long term remission was obtained. One case of malignant lymphoma has been well controled for long time with this diet therapy only.
In despite of the improved procedures of the occult-blood tests of feces, the dieticians frequently meet some difficulties in preparing a menu for the patients, because it is very important to control diets prior to these tests. Some of the vegetables, which are boiled more than thirty minutes, are proved to be available for this purpose. The foodstuffs, which might cause a false positive reaction unspecificially in the tests, should be excluded from this menu. In this experiment, the authors reexamined the influences of cooking for occult-blood tests, using the 68 vegetables and 28 meats, and attempted to magnify the materials in the list of control-diets. Four methods are employed as occult-blood tests; Hematest B, Benzidine test (Adler), Pyramidone method (Adler) and Guajac method (Deen). The occult-blood test, in vitro, are carried out on each food material before and after cooking. The same procedures are done on the feces of healthy volunteers with the same materials. The number of false positive vegetables in all vegetables is markedly reduced after three minutes' boiling. The same result of decrease is obtained on the feces with boiled vegetables, being more prominent in degree than on fresh vegetables only. These results, however, are not obtained on the meats. The present data shows that the control of diets and the method of cooking previously employed for the occult-blood tests are too much exaggerated.