Sixty grams per day of wheat-germ oil, or peanut oil were fed to 17 healthy young girls for a week. Bloods were sampled before, after and during the oil feeding period for a total of 4 times, and the blood samples thus obtained were analyzed for cholesterol content. The fatty acid compositions of both oils were investigated by Gas-Liquid-Chromatography. The serum cholesterol level decreased by 16% in case of the wheat-germ oil and 1% in the peanut oil. Considering the linoleic acid contents of these oils and other oils which effects had been observed in the previous reports, the cause of serum cholesterol lowering effect may be attributed not only to their linoleic acid content, but also to their unsaponifiable matters. Especially the unsaponifiable matters of germ oil for instance, of rice bran oil, corn oil and wheat-germ oil, seem to have a marked cholesterol lowering effect. Further investigation on unsaponifiable matter will be of importance.
We studied what effect the new anabolic agent, stanozolol (17β-hydroxy-17α-methylandrostano-[3, 2-C-] pyrazole) bears on energy metabolism of the aged people. Subjects were seven aged women of under weight. They took one tablet (containing 2mg stanozolol) three times a day, after each meal, for 35 days in late autumn of 1963. Afterwards, for 14 days, they did not take stanozolol. Energy metabolism was measured once a week on basal metabolism by the Douglas bag method. Results obtained were as follows: 1) Energy metabolism had increased about 10% than before dosage. 2) Oxygen-consumption and carbon dioxide-production had increased most after 21 days, respectively 10%, 7%. After that, both of them had decreased. On stop of dosage, they slowed to increase. 3) Non-protein R. Q. decreased, but after the stop of dosage increased again. 4) Rate of combustion of nutrients which provided energy in the body was different after dosage: carbohydrate reduced about 17%, fat increased approximately 10% and protein increased about 7%. 5) The subjects gained average of 1.4kg in weight for 35 days.
It is well known that if the animal take an excess amount of glycine in the diet, a remarkable inhibitory effect on the growth will appear within a short experimental period. In this report, to see an inhibitory effect of an excess amount of glycine on the animals, the rats were fed the diet which containes an excess amount of glycine for the growth test, and at the same time the effect of DL-methionine and L-arginine added to the diet were also observed, 30 pure strain rats, weighing about 65g, were used as the experimental animals.
To see the effect of Excess glycine on the urinary amino acids, free and conjugated form glycine and methionine were investigated by microbioassay method. The fractions of free amino acids were obtained by high voltage paper electrophoresis method. Creatinine in urine was also determined. According to the results, creatinine in urine increased by the excess glycine, and a little more increment was seen by the addition of 0.25% DL-methionine. Free glycine in urine increased greatly by the addition of 5% glycine, but the addition of 0.25% DL-methionine lowered the free glycine excretion in urine. On the other hand, conjugated form glycine also increased when fed 5% glycine diet, and higher increment was appeared by adding 0.25% DL-methionine as seen on Group 4. Free and conjugated form methionine in urine decreased greatly by the addition of 5% glycine. From the figures of free amino acids in urine which were obtained by high voltage paper electrophoresis, an increment of the fractions of basic amino acids were recognized by the addition of 5% glycine in the diet.
There are several reports about the quantity and quality of fats and oils consumed by Japanese people. The present survey was carried out on a certain group of farmers inhabited in a mountainous village in Yamanashi Prefecture in order to know their nutritional status mainly concerning the utilization of fats and oils. The subjects of the survey were 49 families, and their nutrition intakes were, 2142Cal., 65.1g. protein and 24.4g. fat per capita per day. These values were nearly approximate to those of all-Japan average (the National Nutrition Survey). The ratio of fat-calorie to total-calorie was 10.8%. The proportion of animal fat and vegetable oil in the total fat intake was 4:6, the same ratio as in case of Japanese average. Most of the animal fat came from the animal foods, and more than 60% of the vegetable oil they took from the vegetable foods. The only oil food they used was soybean oil. The composition of the fatty acids of fats and oils consumed by them was presumptively evaluated from the figures appeared in some literature.