In the previous reports, the effect of an excess amount of glycine in the diet on the growth of rats, and the excretion of creatinine and amino acids in urine were investigated. In this report, to investigate another critical fact that animal would get fatty liver which is caused by the excess amount of glycine in the diet, at the end of the experimental period the amount of cholesterol in serum, in liver and in adrenal were determined. Serum cholesterol determination was made according to Zak's method and the method of Sperry-Webb et al. was used on the liver cholesterol estimation.
The dietary surveys were carried out once a year from 1962 to 1964, on the families selected from the villagers living in the secluded districts in the Yamanashi Prefecture. From the data of the surveys the following conclusion was elucidated. 1. General nutritional deficiency which is prevalent in the provincial families in Japan was also seen in these surveys, their intakes of the animal protein, calcium and vitamins being, on an average, inadequate comparing with the allowance. 2. It was recognized that the school feeding which was commenced immediately after our first survey was over gave some good effects on the nutrition intake of the school childen, but the dietary conditions of the families was little improved during those two years.