The most limiting amino acid of fish is tryptophan, as is well known, by the report on the composition of essential amino acids which is published by FAO in 1957. According to ‘The Amino acid Composition of Foods in Japan’, however, published by Resources Bureau of the Science and Technology Agency in 1966, the most limiting amino acid of fish varies fish by fish. In this table, for example, the most limiting amino acid of pacific saury is isoleucine and that of flounder is sulfur containing amino acid. In order to clearify the differences of the most limiting amino acid of the fish between FAO's information and that of Japan, we have investigated the supplementary effects of limiting amino acid to the two kinds of fishes, i.e. pacific saury and flounder. The extent of supplementation of the limiting amino acid was planned to raise the protein score and A/E ratio both to ninety or more. As the result, the supplementary effect of tryptophan to each kinds of fishes was higher than that of isoleucine to pacific, and of sulfur containing amino acid to flounder as to the gain of body weigh, the level of plasma protein density, A/G ratio, and the activity of liver xanthin oxidase.
In order to enrich the defatted milk, used in mass feeding of nursery and others, with vitamin B2, and, at the same time, in order to improve its taste, we added B2-butylate, so-called fat-soluble B2, to it, and found that the taste is remarkably improved by adding of more than 0.2mg% of B2-butylate and that the loss of B2-butylate by heating was not observed at all. This result can be considered to reveal a new effect of B2-butylate, unknown to this day.
We performed a survey of the nutritional status of adult men and women, classifying them in farmers, side-job farmers and non-farmers, during 3 days from July 24, 1967 to July 26, in Obara Village, a remote village, under administration of Toyota Health Center, Aichi Prefecture. Our results were as follows: (1) The remarkable feature was the unsufficiency of taking of animal protein, especially, animal and bird meat, and the unsufficiency of taking of milk, which are considered as general nutritional defect characteristic to remote village. This tendency was noted in farmers, side-job farmers, non farmers in order. The difference of taking between professions was statistically significant. (2) In taking quantity of calcium and vitamin A, B1, B2, C etc., we noted remarkable deficit in all profession. From these results we found that the nutritional level classified by profession in Obara Village, Aichi Prefecture, is considerably worse than the nutritional level classified by profession of the national nutrition survey in 1966.
Nutritional values of both school-feeding lunch supplied from the Kagoshima Municipal School-feeding Center and lunch brought from home were investigated at the two middle schools of Kagoshima city. The correlation coefficient between two given nutrients of each lunch above, and the frequency of combination of food-stuffs and of methods of cooking were all obtained. The nutritional pattern of the school-feeding lunch was considerably different from the lunch from home. All the nutrients of the former, except animal protein, were more abundant than the latter, and also calory percent of fat, and the quantity and frequency of using vegetables as food-stuffs showed remarkable higher values in the former lunch. It was, however, notable that the enrichment of vitamin C and calcium at high degree was unavoidable in school-feeding because of these low contents. In the case of the lunch from home, more amount of cow's milk, vegetables and fruits seemed to be recommendable inspite of its abundance of protein-rich food-stuffs.
The management of mass feeding has various difficult problems according to the purpose and character provided. The feeding in the dormitory, one of the industrial mass feedings, is not the same in nutritional administration. This research and investigation was made on the nutritional administration (in the limitative same cost) in seven bachelor dormitories in which had been provided meals by experienced cook without a dietetian. The summary of the same as follows: 1) In the dormitory, where had been provided more than 100 meals, the conditions of nutritive intake was fairly well. 2) In the dormitory which had many employees in proportion to the meals provided in small quantity, the nutritive intake was low. 3) As for the food composition, animal foods intake shows a tendency to increase according to the increase in feeding number. Cereals, especially rice intake was high in the dormitory where had been provided meals in small quantity. In due consideration of the above, in small scale feeding establishments, the nutritional administration must be improved especially on the menu making and the use of the food cost.