Sunflower, Corn, Safflower, Cottenseed, Rapeseed, Soybean, and Sesam oils were given to 25 college girls divided into seven groups for a week. Daily dose of each oil administered in form of French toast was 60g per day for every subject. Cholesterol lowering effects were seen evidently in Sunflower, Corn, and Safflower oils, but no change in other oils.
Six kinds of margarines, of which four were made from only vegetable oils and other two from whale oil, were taken 60g per day for a week, and their effect on serum cholesterol level was observed. Two margarines mainly prepared from whale oil distinctly increased the cholesterol level, but others from vegetable oils only did not give any influence upon its level. Furthermore, one of them which contained the least hydrogenated oil showed even a cholesterol lowering effect. Their fatty acids composition analysed by alkali-isomerization method are shown in table 2.
This experiment was attempted to know how to prevent or reduce the effect of contamination by radioactive substances by fallout. There are many kind of radioactive substances in the fallout, and above all 90Sr and 137Cs seem to be most important. As it has been indicated that the behaviour of Sr in and out of our bodies is very close to that of Ca, the experiment on the relation between the Ca content of diet and the excretion of Sr was performed by using albino rats. Experimental: Adult albino rats weighing about 200g were subjected to this experiment, and they were divided into four groups, the Ist group was fed with a high Ca diet and the 2nd a low Ca diet during experimental period, and the 3rd was fed with these high and low Ca diets alternately for 6 days each during this experiment. 90Sr was injected into the abdominal cavity of the rat, 1.3μc per capita. The 4th group was fed by standard diet and 90Sr was injected together with relatively large amount of non-radioactive Sr. Results: The percentage of total excretion 90Sr during 24 days of the Ist, 2nd and 3rd groups were 15%, 8%, 14% respectively, and of the 4th group was about 25% during 18days. The amount of excretion of 90Sr of high Ca diet group was superior to that of the low Ca diet group, and that of the group non-active Sr injected was the highest of all.
In continuance to the previous report, the relation between Ca intake and 90Sr excretion was investigated with young albino rats. Experimental: 16 immature albino rats weighing about 70g were subjected to this experiment, and they were divided into two groups. The 1st group was fed with a high Ca diet (506mg%) and the 2nd group on a low Ca diet (86mg%). Radioisotope was administered by the single shot method i. e., only 90Sr was injected into the abdominal cavity of the rats, 1μc per capita. After the injection, urine and feces of the rats were collected every two days during the first 10days, thereafter both were collected occasionally. Ten days after the injection, a tip part of the tail of each rat was cut off for the estimation of retained amount of RI in bones, 60-65days after the injection the rats were killed and the one side hind leg, incisor were taken off for determination of deposit activity in hard tissue. Results: The amount of 90Sr excreted by the rats fed on the high Ca diet were higher than that of the low Ca fed group. The retained amount of 90Sr in hard tissue, especially, in the teeth was larger in the low Ca group than that of the high Ca group.
This study was projected for the purpose of knowing how to reduce the 90Sr excretion from relation between amount of Ca and Mg intake. Experimental: Twenty-four growing albino rats were subjected to this experiment, and they were divided into four groups. The Ist group was fed a low Ca-normal Mg diet and the 2nd a low Ca-high Mg diet and the 3rd high Ca-normal Mg diet and the 4th high Cahigh Mg diet. The high Mg means to contain twice amount of Mg in the normal diet. Radioactive Sr (Sr-90) was injected into the abdominal cavity of the rat, 5μc per capita. After the injection, urine and feces of the rats were collected every two days during the first 10 days, thereafter both were collected occasionally up to the thirtieth day. Thirty days after the injection the rats were killed and under leg, incisor were taken off for determination of deposit activity in hard tissue. Results: (1) The amount of 90Sr excreted by the rats fed the high Ca diet was higher than that of the low Ca fed group. (2) In high Mg group, 90Sr excretion in urine seemed to indicate higher trend than normal Mg fed group. (3) The retained amount of 90Sr in hard tissue, especially, in the femur was larger in the low Ca group than that of the high Ca group. (4) In the report 2, the retained amount of 90Sr in the teeth was larger than the under-leg. This different is presumable due to the duration to be anatomized. (5) The high amount of Mg uptake had no relation to depesiticn of 90Sr, whereas it had in the case of high Ca uptake.
It has been well recognized that during the analytical process of the amino acids by ninhydrin color-imetric method, the time of heating and the temperature has a remarkable influence on the experimental results. This paper is to report about the effect of heat and time on the values of amino acids which were extracted with solvent and developed color by heating with ninhydrin after separation by paper electrophoresis.
Quantitative Analysis of the amino acids by Highvoltage-paper electrophoresis was described in our recent reports. This time, analysis of lysine and arginine in some foods and proteins was made by using this method.