This report is concerned with fourth-annual survey conducted from 1955 on 119 farmers of two villages in Iwate Prefecture, one lying at the foot of Kitagami mountainrange and the other on the coast of the Pacific Ocean. This survey was carried out twice with the same subjects, in the summer and winter of 1958, and effects of nutrients and drugs administered for a period of six months were investigated. (1) Biometrically, body build of subjects in general represented a typical heavy muscular worker type. (2) The basal metabolic rate was pretty hight amounting to 110%. (3) Haemoglobin and serum protein levels were within the normal range. Serum cholesterol concentration ranged around 200mg./dl. Natrium concentration in serum exceeded a little above the normal level. Protein bound iodine in serum showed a high level within normal limit. (4) Excretion of nitrogen was about 10g per day, and those of creatinine and 17-ketosteroid were also in lower levels than usual. (5) Intake of almost all nutrients except carbohydrate and vitamin C was below the standard level. (6) Among these nutrients and drugs administered, methyltestosteron given to old males had most effectiveness, cornoil, pantothenic acid, lecithin, B1 following.
Ten College girls were subjected to the experiment to clarify the relationship between the ingested foods and amino acids in blood and in urine. The four important amino acids, lysine, threonine, methionine and tryptophan in plasma as well as in urine were determined microbiologically, and the relationship between these amino acids and biochemical status of protein were searched for, and the results were as follows: 1) Tryptophan intake was not generally sufficient and limiting factor of the protein score was, in all subjects, tryptophan, the score being 77.8 on an average. 2) There were no direct correlation between the ingested foods and any of the four amino acids analyzed in plasma, however, the subjects who ingested much protein showed high level of plasma methionine and threonine, whereas subjects who took less protein showed a tendency of low level of all amino acids. 3) As to the excretion of the amino acids in urine, tryptophan and methionine showed significant correlation to the ingested amino acids. 4) When whole day urine were divided into 4 parts, morning urine, afternoon urine, night urine, and sleeping time urine, the amino acid excretion were generally low in the morning and high in the night, with exception of methionine which was lowest in the sleeping time. This hourly variation was greater (about 20-30%) in lysine and threonine, and it was smaller (about 15%) in methionine and tryptophan.
This paper is to report about the results of the further research for the relationship between the ingested foods and the amounts of amino acids in plasma and in urine. Thirty widows who live with children in poor economic state were selected as a low-income group, and sixty-four farmers, male and female, were also subjected as suburban people. Their food intake was surveyed in detail and as accurately as possible, and four kinds of amino acids in plasma and in urine were determined microbiologically. Correlation between the food composition and the amino acids in blood and in urine were observed as follows: 1) The quality of protein ingested by the low-income women were inferior to that of the College girls previously surveyed, and protein score was lower. 2) There was no definite correlation between the ingested protein or amino acids and clasma amino acids, but methionine and its protein score were correlated slightly. In case of farmers, there was significant difference between men and women about the tryptophan level in plasma. 3) Between the excretion and ingestion correlation was recognized in typtophan. Man showed significantly higher excretion of tryptophan than woman.
To summerize this series of reseach concerning to the relationship between the ingested foods and amino acids in blood and in urine the author tried to deduce some clearer knowledge by discussing furthermore. The results of the discussion were: 1) There was found no close relationship between the plasma levels of the amino acids and those ingested. 2) Between the ingested tryptophan and excreted one there was correlation in all women subjects, and in case of college girls methionine also showed significant correlation. When all women subjects were divided into two groups according to the levels ingested, ingestion and excretion was paralled in all amino acids. 3) The whole day urine of the college girls was divided into 4 parts and the state of the amino acids of each part were observed separately. The values of lysine and threonine were higher in the night and the methionine and tryptophan showed the different tendency, decreasing in the sleeping time. 4) The level of Hemoglobin was also correlated significantly to the amino acid ingested.
An evaluation of the essential amino acid intake was conducted on the data of the Nutrition Survey carried out in the Japanese fishing village, 1959, and the results obtained were summarized as follows: 1) An average daily intake of protein was 89.1g (man value), about 10% higher than the standard value, and any of the essential amino acid intake was greater, as compared with the value calculated from the Standard Food Construction of Japanese people. As for Lysine and Methionine, more than 50% of each of them was supplied with animal foods (Fish and Fishshell), and so their intake showed an upward tendency with the increase of animal protein coefficiency. 2) In comparing the intake of the essential amino acid with the Provisional Protein Pattern (FAO), the intake of, Methionine, Sulfurcontaining amino acid, and Tryptophan was always low, their mean percentage being, 85, 78, and 70 respectively. Especially the intake of Tryptophan was lowest, the first limiting amino acid; this may be attributed to the fact that animal protein was supplied most with fish and fishshell, and least with eggs and meats.
Seasonal variation of the growth of body weight of infants was investigated by 928 babies born in Tokyo city. The greatest increase of body weight till 6th month after birth was shown by both boys and girls born in spring, while that of the growth till 12th month after birth was sbown by boys born in spring and girls born in summer.