Since the discovery of a High voltage-paper electrophoresis method for the separation of the amino acids by Michl in 1951, it has been recognized as a quite effective method for the analysis of the amino acids. In this report, a very simple apparatus for this purpose was used and satisfactory results was obtained on the quantitative analysis of lysine, histidine and arginine. The advantage of this method is; by using rather long filter paper on the apparatus, a clear diagram of separated amino acids can be obtained in a short time.
Of quantitative Analysis methods for the amino acids which had been isolated in various ways, a ninhydrin method is known as the most common and effective one. In this report, as a continuous experiment of our recent paper, a colorimetric ninhydrin method is used for the quantitive analysis of lysine, arginine and histidine, after the separation by Highvoltage-paper electrophoresis.
The thiamine actions were investigated about thiamine anhydride sulfoxide (TAO) and thiamine anhydride sulfone (TAO2) which were obtained by oxidating thiamine anhydride with H2O2. It was found that though 500λ of TAO and TAO2 were respectively administrated to the rats which were feeded with thiamine deficient diet, each substance did not show the thiamine action and that LD50 of TAO2 was lowe than that of thiamine.
Both thiamine anhydride (TA) and thiamine anhydride sulfone (TAO2) obtained by oxidation of TA did not show the biological activities. It was studied whether that survival rate was low when the TAO2 action was tested. When 500λ of TAO2 in the presence of 5λ of B1 was administrated to rats, TAO2 did not show antimetabolic action.