The aim of this experiment was to investigate into the influences of various soluble oxalic salts upon the utilization of a calcium source. Feeding albino rats with diets containing 0.3% calcium (CaCo3 was used as calcium source) with potassium oxalate, sodium oxalate, ammonium oxalate as 1% oxalic acid added, we investigated into there calcification. These salts showed the same interference as 1% oxalic acid. Oxalic acid, whether in free acid or in soluble salts, reduced the utilization of calcium when its amount exceeded 1% of diet.
The aim of this experiment was to examine the availability of calcium oxalate used as a calcium source. The result was showid, when it was used as a calcium source it showed normal calcification. And it was described, the discussion and the summary on the report II.
A micro-colorimetric method for the determination of inorganic phosphorus in the biological fluids and serum phosphatase is described. Its use as a rapid and convenient method for estimating the serum inorganic phosphorus and alkaline phosphase of the rachitic children is of particular value.
To remove the odour of imported rice and to fortify it with vitamins at fat, the following method was studied. Afer mixing vegetable oil with aqueous solution of vitamin B1 kneaded it with rice bran, then mixed it with imported rice, and left alone at room temperature for about 10 hours. By the procedure, we obtained the deodourated rice which is contained 1.7 percent of neutral fat and 100 percent of vitamin B1.
The recovery process of the lower basal metabolism after the war was reported in report I (Jap. J. Nutr., 10, 108, 1952), which was based on the data measured between August 1949 and 1951. After that the measurement has been continued up to present. This report gives an acconunt of the process of the basal metabolism measured after that until December 1953. The reusults were as follows: 1) The B. M. R. which was in lower level until August 1950 began to rise and it kept the constant level there after, as shown in figure 1 and 2. The B. M. R. for subsequent three years between 1950 and December 1953 were averaged into 33.7Cal./hr./sq.m. in subject S. S. and 33.3 in S. N.. These values are 4 and 6% higher than the lower B. M. R. in preceeding years respectively. 2) The recovery of the declined B. M. R. needed about two years after the latter of 1948. when both sujects could begin to get diet of the nutritional allowances. It indicates that the recovery of the declined B. M. R. in consequence of prolonged food shortage needs a few years long to reach normal. 3) The seasonal variation of the basal metabolism in this data showed that in winter was 5% higher and in summer 4% lower than the average. 4) Respiratory quotient showed the seasonal variation: in summer higher than in winter in the subiject S. N. whose body weight was constant through a year, but it was not clear in the subject S. S. whose boby weight showed a seasonal change.
In orde to confirm how glycogen is formed from fat in the body of a living thing, we have made some expriments in feeding white rats on butter, glycerin, and fattyacid and unsaturated fattyacid contained in butter. 1. A rat fed on 70 percent butter increased in weight 3.7 grams on an average in ten days. When starch was used instead of fat, its weight showed an increase of 2.6 grams on an average in ten days. It is evident that butter is far superior to starch. 2. The quantity of glycogen contained in the liver of a rat fed on butter is 1.69% on an vaerage and is far more than that of glycogen in the liver of a rat fed on starch, which is 1.38%: the fat contained in butter changes more easily to glycogen than starch. 3. On the fattyacids and glycerin contained in butter what contributes most to an increase of the glycogen in a liver is oleicacid Na., whose percentage is 1.035, and what do least are stearinacid Na., whose percentage is 0.309, and glycerin, whose percentage is 0.28. 4. An injection to a rat of nearly the same quantity of linolacid, linoleinacid or arachidonacid as contained in butter makes no increase in glycogen. 5. What can be turned from fattyacid into glycose is saturated fattyacid alone except oleinacid, and between an increasing amount of glycogen and the number of carbon in the given fattyacid there seems to be something correlative.