In orde to confirm how glycogen is formed from fat in the body of a living thing, we have made some expriments in feeding white rats on butter, glycerin, and fattyacid and unsaturated fattyacid contained in butter.
1. A rat fed on 70 percent butter increased in weight 3.7 grams on an average in ten days. When starch was used instead of fat, its weight showed an increase of 2.6 grams on an average in ten days. It is evident that butter is far superior to starch.
2. The quantity of glycogen contained in the liver of a rat fed on butter is 1.69% on an vaerage and is far more than that of glycogen in the liver of a rat fed on starch, which is 1.38%: the fat contained in butter changes more easily to glycogen than starch.
3. On the fattyacids and glycerin contained in butter what contributes most to an increase of the glycogen in a liver is oleicacid Na., whose percentage is 1.035, and what do least are stearinacid Na., whose percentage is 0.309, and glycerin, whose percentage is 0.28.
4. An injection to a rat of nearly the same quantity of linolacid, linoleinacid or arachidonacid as contained in butter makes no increase in glycogen.
5. What can be turned from fattyacid into glycose is saturated fattyacid alone except oleinacid, and between an increasing amount of glycogen and the number of carbon in the given fattyacid there seems to be something correlative.
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