A development of laser-excited microscopic fluorescence measuring system and the experi-mental results of observed spectra of the cancer cells, in vivo, such as human acute myeloid leukemias and human uterine cancer cells, etc. are reported in the present paper. The measuring system is capable of illuminating only one cell with a laser-probing beam of approx-imately 10μm diameter and observing its microscopic fluorescence spectrum. The sample-cell is previously vital stained with acridine orange or hematoporphyrin dye solution, The resul-tant characteristic spectra can yield us with various information regarding biopolymers at a biocell level, in particular the basic data for the spectroscopic diagnosis of cancer cells.
A precision humidity generator was manufactured in order to establish the humidity standard. This generator can make air of constant humidity from about 10 to 100% relative humidity and with an accuracy of 0.2%. The paper describes the principle of generation, the structure of instrument, the process of handling, the temperature and the humidity stability and uniformity in the test chamber and so on. The principal features of the generator are given as follows; (1) The two-pressure and the two-temperature methods are used at the same time to extend the humidity range. (2) The pre-saturator is added to complete the saturation and to stabilize the temperature. (3) The test chamber is immersed in the thermostatic water bath whose temperature is controlled within 10mK. (4) The measurement of pressure and temperature and the calculation of humidity are automatized.