Effect of gallium nitrate on the pharmacokinetics and tissue accumulation of67Ga was investigated in rats bearing turpentine oil-induced abscess. Gallium nitrate accelerated the blood disappearance of67Ga, but this effect was less potent than that of ferric nitrate. The accumulation of67Ga in liver was significantly lowered by gallium nitrate, but no significant decrease of67Ga accumulation in abscess was observed. On the other hand, 67Ga accumulation in bone was slightly facilitated by gallium nitrate. Ferric nitrate exerted a similar effect on tissue accumulation of67Ga as gallium nitrate.
The γ-ray spectrum analysis program by the personal computer (NEC PC-9801 series) for the instrumental neutron activation analysis (INAA) has been developed. The γ-ray peaks of the spectrum are searched automatically by inputing analytical conditions. The area of γ-ray peaks is analyzed by means of a fitting technique based on a non-linear least squares method. As a result, peaks, energies, and their intensities are calculated. Their intensities can be corrected for decay of the corresponding nuclide. Contents and concentrations of elements of interest in the sample are calculated by comparison with the intensitities of standard samples. The overlaping multiple peaks have been analyzed precisely and easily by this program.
Quantification of HBs-antibody assay was carried out using a commercialised assay kit and standard solutions of HBs-antibody recognised as 1 St reference preparation of hepatitis B immunogloblin by WHO. Standard curve of HBs-antibody was drawn with the function of 3D-spline and the correlation factor was obtained as r=0.999. Coefficient of intra-assay variance was 3.8% and that of inter-assay variance was 7.8%. Dilution tests showed satisfactory results in the range of 2-16 times. Correlation between value of cut-off indices and concentration of HBs-antibody was obtained as the formula of y=2.599 x -3.894 (r=0.992) and 2.1 of cut-off index corresponded to about 5 mIU/ml of HBs-antibody concentration.