In a previous paper, we studied elimination and presence rates of nuclides injected in irradiated mice to investigate metabolic mechanism of several essential metals in injured mice leading to recovery after sublethal irradiation, and it was observed that the elimination and the presence rates of elements in tissues and organs showed a difference between the doses, particularly for59Fe in liver and blood of mice. In this paper, further observations with biochemical states of59Fe in the blood of irradiated mice are described as well as with distribution of59Fe in the blood. An accumulation of59Fe to erythrocytes was remarkably decreased by the irradiation, and a big radioactive peak (P-1) was seen in a profile of gel filtration of hemolysates in both unirradiation (control) and irradiation samples 2 days after the irradiation. At 8 days after the irradiation, the presence rate of59Fe in the erythrocytes increased, and in the profile of gel filtration, for 10 Gy irradiation, the peak P-1 decreased and another radioactive peak was observed, but the difference between 4 Gy and control was not clear. These results suggested a metabolic process leading individuals to recovery from injury after irradiation.
In a previous paper, in order to investigate metabolic mechanism of several essential metals in injured mice leading to recovery after sublethal irradiation, we studied on elimination and presence rates of nuclides injected to irradiated mice, and we observed that the remarkable increasing of presence rate of59Fe in liver by the irradiation. In this paper, biochemical states and distribution of59Fe in liver of mice exposed to γ-rays of 4 Gy or 10 Gy were observed. Two main radioactive peaks (P-1 and P-2) were observed on the gel filtration profile of the supernatant of homogenized liver of unirradiated mouse, at 2 days after the irradiation, P-2 decreased clearly. However, at 8 days after the irradiation, proportion of P-2 increased, and this increasing of P-2 may be closely related to the recovery process from injury after the irradiation. Furthermore, SDS-polyacrylamide gel electro-phoresis analysis of P-1 and P-2 showed difference between doses. In addition, the distribution rates of59Fe in main protein compositions of liver were different between doses and days after the irradiation, and it suggests the influence of the irradiation to iron-binding proteins.