The microflora of the fish, Trachurus trachurus (Linné), was studied before and after storage at 7°C. Before storage, the skin slime mainly contained Pseudomonas and Vibrio in summer, and Achromobacter and Pseudomonas in winter. During storage, Pseudomonas and Vibrio were predominant on the skin surface where Achromobacter, Comamonas, Micrococcus, Corynebacterium, and Flavobacterium decreased or disappeared. After storage for a few days, Pseudomonas and Vibrio increased from 30-62% to 96-98%, oxidasepositive bacteria from 42-69% to 100%, and penicillin-resistant bacteria from 28-64% to 93-107%. Vibrio was usually predominant in the microflora of the gut contents before and after storage in summer, as well as in winter. The results also suggest that the oxidase-positive organism might be used as an indicator in the quality test of fresh fish.
TwoS-triazine compounds, ammeline and melamine, were isolated from some fish meal which had caused blindness of baby chicks. A toxic substance was extracted from the meal by fractio- TwoS-triazine compounds, ammeline and melamine, were isolated from some ffsh meal which had caused blindness of baby chicks. A toxic substance was extracted from the meal by fractionation and hydrostatic settling in organic solvents. Further extraction was performed by hydrochloric acid solution and dimethylsulfoxide solution of sodium carbonate. Feeding experiments were conducted on baby chicks with melamine, ammeline, and four compounds related to them. It was proved that blindness of chicks was caused by the administration of feeds containing ammeline. This substance was added to fish meal for the purpose of increasing the nitrogen content of commercial chick feeds.