From the blood and liver of an acutely dead Japanese crane with inclusion body disease, was isolated a virus actively propagating in fibroblasts from chicken or duck embryos with formation of intranuclear inclusion bodies. The viral isolate was filterable through a 220 nm membrane filter but not through a 100nm filter. It was sensitive to ether and viral propagation was depressed by IUDR, and was inactivated by heating at 56°C for 10min. The isolate did not agglutinate chicken and goose erythrocytes and crossly reacted with the x/87/79 strain of crane herpesvirus by cross neutralization test. Neutralizing antibodies against the crane herpesvirus were detected in two birds each of three crane species contacted with the dead case.
Out of 70 calves with retarded growth or respiratory disorders, which were from 28 farms in Hyogo Prefecture and slaughetred in 1994 and 1995, 47 (67.1%) and 64 (91.4%) were shown to have inflammatory cranial to caudal lobes and hepatized lung lesion, respectively. From these lesions, Pasteurella multocida (64.3% calves), Haemophilus somnus (40.0%), Mycoplasma spp.(34.3%), Fusobacterium necrophorum (21.4%), Actinomyces pyogenes (21.4%), Branhamella spp.(11.4%) and P. haemolytica (10.0%) were isolated under a sufficient moisture condition. In aerobic cultures of freshly producted lesions. H. somnus, Mycoplasma spp. and A. pyogene were predominant.
A 5-year-old Holstein cow was shown to have a tumor mass 20cm in diameter in the subcutis of the ventral neck around the larynx, with significantly high serum T4 and T3 levels. Neither bacteria nor neoplastic cells were detected from bloody serous content of the mass. Symptomatic therapy for additional diagnoses of vagus indigestion and renal failure, was not effective. Postmortem histopathology of the mass revealed active proliferation of atypical follicular cells with follicle formation and invasive growth to the surrounding, tissues.
Canine red blood cells (RBC) frozen and preserved in the presence of hydroxyethyl starch (HES), were shown to have ability to produce 2, 3-diphosphoglyceric acid (2, 3-DPG) and adenosine 5'-triphosphate (ATP) after thawing, although the recovery rate of hemoglobin (Hb) was slightly lower than RBC frozen with glycerol (GL). When the HES-frozen RBC were exposed to physiological osmotic pressure after thawing without removing the cryoprotectants, 71% of Hb was recovered, whereas GL-frozen RBC were totally hemolysed. These results suggested that HES would be a clinically useful washing-free cryprotectant for blood transfusion.
Extra-articular fixation was performed using nylon line (fishing leader No.200) and sleeve in canine and feline cases of anterior cruciate ligament rupture. Two dogs showed weight-bearing with the affected leg by 2 days postoperation and the lameness almost disappeared in one month. In a feline case the lameness of the affected leg disappeared at 5 days postoperation. The elongation rate of the nylon ring formed was lesser in using a sleeve than in manual knotting while the maximum tensile strength was similar in both cases.
Two cats with oral pain, dental plaque and calculi, and severe gingivo-stomatitis (GS) were admitted. After scaling performed some months before, cats were treated by intermittent administration of antibiotic, steroid, and lactoferrin. GS was decreased in severity during the drug administrations, but became worse again after discontinuation of the treatment. Then total tooth extraction was performed, resulting in the reduction of oral pain and GS, and satisfactory convalesence was seen in the both cases.
From the feces of 14 of 500 (2.8%) adult cattle brought to a slaughterhouse in Shizuoka prefecture from September to December 1995, Cryptosporidium oocysts were detected. Most of the positive cattle belonged to a particular farm, and the presence of oocysts was not related to clinical diarrhea. The oocysts isolated by sucrose floatation were identified as Cryptosporidium muris after modified Kohn's and Kinyoun's acid fast stains. The infection was established in mice by oral administration of the isolated oosysts.